Publications by authors named "Zhen-Fu Fang"

Article Synopsis
  • A rare plant species in Yun-xiao County, Fujian Province, has a critically low population consisting of only 25 individuals, mainly young seedlings, and just one mature mother tree.
  • The spatial distribution shows most seedlings are clustered within 10-25 meters of the mother tree, indicating an unsustainable population due to lack of older trees and intense competition from surrounding species.
  • An evaluation of this plant's conservation status reveals it is classified as "extremely endangered" with high protection value and priority, suggesting immediate conservation actions are necessary.
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Mouse embryonic stem (ES) cells can be differentiated into neural lineage cells, but the differentiation efficiency remains low. This study revealed two important factors that influence the neural differentiation efficiency of mouse ES cells: the first is the quality of embryonic bodies (EBs); good quality of EBs consistently originated from a suspension culture of 1x10(5) ES cells/ml serum-free chemically defined neural inducing medium and they exhibited a smooth round shape, with a dark central region surrounded by a light band. Such EBs are capable of attaining high neural differentiation efficiency.

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Six human embryonic stem cell lines were established from surplus blastocysts. The cell lines expressed alkaline phosphatase and molecules typical of primate embryonic stem cells, including Oct-4, Nanog, TDGF1, Sox2, EBAF, Thy-1, FGF4, Rex-1, SSEA-3, SSEA-4, TRA-1-60 and TRA-1-81. Five of the six lines formed embryoid bodies that expressed markers of a variety of cell types; four of them formed teratomas with tissue types representative of all three embryonic germ layers.

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