Enhancing antitumor activity of herceptin in HER2-positive breast cancer cells: a novel DNMT-1 inhibitor approach.

HER2 antagonists remain the cornerstone of therapy for patients with HER2-positive breast cancer. This study introduces a novel small-molecule inhibitor of DNA methyltransferase 1 (DNMT-1), referred to as DI-1, designed to synergize with HER2 antagonists in treating HER2-positive breast cancer cells. Clinical data reveal a negative correlation between DNMT-1 expression and PTEN levels, and a positive correlation with the methylation rates of PTEN's promoter.

Metadherin inhibits chemosensitivity of triple-negative breast cancer to paclitaxel via activation of AKT/GSK-3β signaling pathway.

Triple-negative breast cancer (TNBC) has an aggressive clinical course, and paclitaxel (PTX)-based chemotherapy remains the main therapeutic drug. Metadherin (MTDH) acts as an oncogene that regulates proliferation, invasion, metastasis, and chemoresistance. This study aimed to investigate whether TNBC chemosensitivity to PTX was related to the MTDH/AKT/glycogen synthase kinase-3beta (GSK-3β) pathway.

Calcium dobesilate prevents PLD-induced hand-foot syndrome by alleviating capillary endothelial tight junction injury via the HA/CD44 pathway.

Pegylated liposomal doxorubicin (PLD) has excellent therapeutic efficacy in the treatment of cancers, but can cause serious adverse reactions such as hand-foot syndrome (HFS). Our previous research suggests that both PLD-induced HFS may be associated with injury to tight junctions (TJs) in the skin and that calcium dobesilate (CaD) can alleviate HFS. However, the underlying molecular mechanism is not well understood.

Body Mass Index at Diagnosis as a Prognostic Factor for Early-Stage Invasive Breast Cancer after Surgical Resection.

Background: The aim of this study was to investigate the association between body mass index (BMI) and prognosis of Chinese women with breast cancer.

Patients And Methods: 3,380 primary breast cancer patients who underwent surgery from 2010 to 2012 were selected and classified as low BMI group (BMI < 25.0) and high BMI group (BMI ≥ 25.

[Proteomic identification and comparison of differentiation-related proteins in gastric carcinoma cell lines].

Objective: To investigate the differentiation-related proteins in human gastric carcinoma cell lines by comparative proteomics.

Methods: The holoproteins of human gastric carcinoma cell lines MKN28, SGC7901 and BGC823 were measured by two-dimensional gel electrophoresis and matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Some proteins identified by proteomics were tested by Western blot in the cell strains and tissues of gastric carcinoma.

[Relationship of cyclooxygenase-2 and multidrug resistance associated factors to chemosensitivities in gastrointestinal carcinomas].

Objective: To investigate the relationship of cyclooxygenase-2 (COX-2), p-glycoprotein(P-gp), glutathione S-transferase-pi (GST-pi), and topoisomerase II alpha (Topo II alpha) to chemosensitivities in gastrointestinal tract carcinomas.

Methods: The tumor tissue samples were collected from 84 specimens of gastrointestinal carcinomas. The expressions of COX-2, P-gp, GST-pi, and Topo II alpha were determined immunohistochemically.

[Effect of ulinastatin on intestinal mucosal barrier function of rats with obstructive jaundice].

Objective: To investigate the effect of ulinastatin on intestinal mucosal barrier function of rats with obstructive jaundice.

Methods: Seventy-two male SD rats were randomly divided into sham operation, obstructive jaundice, and ulinastatin treatment groups (groups A, B, and C, respectively). In groups B and C, the common bile duct was ligated to induce obstructive jaundice.

[Detecting bone marrow micrometastasis of gastric cancer by magnetic activated cell sorting combined with fluorescent activated cell sorting].

Background & Objective: Several methods are used to detect bone marrow micrometastasis of gastric cancer with different accuracies. In breast cancer, tumor cells in blood can be detected sensitively and specifically by magnetic activated cell sorting (MACS) and fluorescent activated cell sorting (FACS). This study was to investigate the clinical value of this method in detecting bone marrow micrometastasis of gastric cancer.

[Drug distribution in gastric cancer and adjacent tissues by preoperative intraperitoneal chemotherapy with Co-fluorouracil liposome].

Objective: To examine the distribution of fluorouracil in gastric cancer (CA), lymph node (LN), normal gastric mucosa (NG), peritoneum (PE), greater omentum (GO) and lesser omentum (LO) by preoperative intraperitoneal chemotherapy with Co-fluorouracil liposome (Co 5-Fu), and offer an experimental basis for clinic practice.

Methods: Ninety-six gastric cancer patients were divided into four groups: Co 5-Fu i.v.

[Multi-antibody combined determination of lymph node micrometastasis in patients with gastric cancer].

Background & Objective: Immunohistochemical staining is a simple method for determination of lymph node micrometastasis in gastric cancer; but the sensitivity is low. Whether this disadvantage can be improved using multi-antibody combined determination is still controversial. This study was designed to determine the lymph node micrometastasis in patients with gastric cancer by the antibodies of cytokeratin 20 (CK20), epithelial membrane antigen (EMA), and carcinoembryonic antigen 72-4 (CA72-4)for clarifying the value of multi-antibody combined determination of micrometastasis.

[Relationship between expression of multiple tumor suppressor(MTS1) and E-cadherin and metastasis of breast carcinoma].

Background & Objective: Most of breast cancer patients died of distant metastasis. There is still not a method of predicting and early diagnosing the metastasis. This study was conducted to investigate the relationship between the expression of multiple tumor suppressor 1(MTS1) as well as E-cadherin and metastasis in breast cancer.