J Mol Endocrinol
February 2021
RFX6 transcription factor is believed to play a central role in directing cell development of insulin-producing pancreatic islet. RFX6 homozygous mutations cause syndromic neonatal diabetes with hypoplastic pancreas. However, RFX6 heterozygous mutations cause maturity-onset diabetes of the young (MODY) with normal pancreas development.
View Article and Find Full Text PDFPancreatic endocrine islets are vital for glucose homeostasis. However, the islet developmental trajectory and its regulatory network are not well understood. To define the features of these specification and differentiation processes, we isolated individual islet cells from TgBAC(neurod1:EGFP) transgenic zebrafish and analyzed islet developmental dynamics across four different embryonic stages using a single-cell RNA-seq strategy.
View Article and Find Full Text PDFTo explore the roles of three novel genes BM390716, BI274487 and AA963863 and the correlation between them during liver regeneration of rats, eight kinds of liver cells were isolated using the combined percoll density gradient centrifugation and immunomagnetic bead method. Rat genome 230 2.0 array was used to detect the changes in expression of genes involved in metabolism of extracellular matrix and the novel genes in rat genome.
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