Genome-Wide Analysis of Soybean Apyrase Gene Family and Functional Characterization of GmAPY1-4 Responses to Aluminum Stress.

Apyrases (APYs) directly regulate intra- and extra-cellular ATP homeostasis and play a key role in the process of plants adapting to various stresses. In this study, we identified and characterized soybean APY (GmAPY) family members at the genomic level. The results identified a total of 18 APYRASE homologous genes with conserved ACR domains.

Taming Strong Metal-Support Interactions to Generalize Gold-Zinc Oxide Catalysts in Oxidative Coupling.

Strong metal-support interactions (SMSI) are at the cutting edge of catalysis research, yet their size-dependent nature remains both widespread and subject to ongoing debate. Here, we report the discovery of bell-shaped size-dependent SMSI, and we establish its structure-SMSI-performance relationship in oxidative C-H/O-H coupling reactions. Using Au/ZnO as a prototypical catalyst, we develop a thermodynamic equilibrium model that quantitatively captures the size-dependent surface energy and tension disparities, identifying the particle size ratio as the descriptor for bell-shaped encapsulation dynamics.

Berberine disrupts the high-affinity iron transport system to reverse the fluconazole-resistance in Candida albicans.

Invasive fungal infection is usually caused by Candida albicans infection, which has a high incidence rate and mortality in critically ill patients. New drugs are needed to combat this pathogen since the limited treatment options currently available and increasing resistance to existing drugs. Berberine (BBR) is an active compound in Coptis chinensis, Phellodendron chinense and Radix berberidis, which is clinically used to treat inflammatory bowel disease, but its inhibitory effect on drug-resistant fungi has not been clarified.

Analysis of the Volume of the Liposuction-Derived Adipose Tissue in Clinical Application.

Introduction: The strategy of adipose component transplantation has been proposed and widely used in both reconstructive and aesthetic surgery. However, there is no uniform standard for the preparation of component fat, and the volume calculation of liposuction and injection in clinical applications is mostly based on experience. This study aims to analyze the volume of component fat obtained during clinical series.

Evaluation of the association between lymph node ratio and long-term survival in patients after surgery for lymph node-positive bladder cancer: a SEER population-based study with external validation.

Background: The lymph node ratio (LNR) has been recognized as an emerging prognostic biomarker in various malignant tumors. Our study aimed to investigate the prognostic role of LNR in postoperative patients with lymph node-positive bladder cancer.

Methods: This study comprised a total of 3911 eligible patients diagnosed with lymph node-positive bladder cancer.

Improving Anti-Corrosion Property of Aluminium Alloy by Fabrication MAO Coating via Mixing Titanium Potassium Oxalate into Electrolyte.

Titanium potassium oxalate had been mixed into the electrolyte to improve the anti-corrosion property of the micro arc oxidation coating on the surface of the aluminium alloy. The surface and cross-section of the coating at different titanium potassium oxalate concentrations had been observed by scanning electron microscopy, showing that when the titanium potassium oxalate concentration was 10 g/L, the coating compactness was better. Additionally, the element content of the coating had been studied by the energy dispersive spectrometer, and results proved that the coating consisted of Al, O, Ti, Si, and P.

UiO-66 with missing cluster defects for high-efficient extraction and enrichment of benzoylurea insecticides.

The creation of defects in crystalline structures can tune metal-organic frameworks (MOFs) properties, such as improving their adsorptive and catalytic performance with producing more porosity and active sites. In this work, the bimetallic UiO-66 containing Zn and Zr was prepared. And then UiO-66 with missing cluster defects (UiO-66-1/3) were obtained by acid washing to remove the Zn nodes.

Rectus Femoris Muscle Segmentation on Ultrasound Images of Older Adults Using Automatic Segment Anything Model, nnU-Net and U-Net-A Prospective Study of Hong Kong Community Cohort.

Sarcopenia is characterized by a degeneration of muscle mass and strength that incurs impaired mobility, posing grievous impacts on the quality of life and well-being of older adults worldwide. In 2018, a new international consensus was formulated to incorporate ultrasound imaging of the rectus femoris (RF) muscle for early sarcopenia assessment. Nonetheless, current clinical RF muscle identification and delineation procedures are manual, subjective, inaccurate, and challenging.

Correction to: Marker Ki-67 is a potential biomarker for the diagnosis and prognosis of prostate cancer based on two cohorts.

[This corrects the article on p. 32 in vol. 12, PMID: 38292624.

Phytochemical, pharmacological, pharmacokinetic and toxicological characteristics of : a review.

(ZSS) is the seeds of (Bunge) Hu ex H. F. Chou, which has the effects of nourishing heart and liver, tranquilizing heart and tranquilizing mind.

Development of a centrosome amplification-associated signature in kidney renal clear cell carcinoma based on multiple machine learning models.

Background: Centrosome amplification (CA) has been shown to be capable of initiating tumorigenesis with metastatic potential and enhancing cell invasion. We were interested in discovering how centrosome amplification-associated signature affects the prediction of prognosis and response to therapy in kidney renal clear cell carcinoma (KIRC).

Methods And Materials: The TCGA-KIRC dataset was used to construct a centrosome amplification-associated signature using the random survival forest analysis and Cox regression analysis, and the ICGC and GEO datasets were employed for signature validation.

Quantitative neuromuscular monitoring with train-of-four ratio using sonomechanomyography (SMMG): Toward more sensitive evaluation of neuromuscular blockade.

In the post-anesthesia care unit, there is a high occurrence of residual neuromuscular blockade, which puts patients at risk of negative consequences such as hypoxia. Assessment based on the train-of-four ratio (TOFR) has been used to avoid residual neuromuscular blockade when the TOFR is greater than 0.9, measured at the adductor pollicis muscle (APM).

Functional Monitoring of Patients With Knee Osteoarthritis Based on Multidimensional Wearable Plantar Pressure Features: Cross-Sectional Study.

Background: Patients with knee osteoarthritis (KOA) often present lower extremity motor dysfunction. However, traditional radiography is a static assessment and cannot achieve long-term dynamic functional monitoring. Plantar pressure signals have demonstrated potential applications in the diagnosis and rehabilitation monitoring of KOA.

Planting density effect on poplar growth traits and soil nutrient availability, and response of microbial community, assembly and function.

Background: The interaction between soil characteristics and microbial communities is crucial for poplar growth under different planting densities. Yet, little is understood about their relationships and how they respond to primary environmental drivers across varying planting densities.

Results: In this study, we investigated poplar growth metrics, soil characteristics, and community assembly of soil bacterial and fungal communities in four poplar genotypes (M1316, BT17, S86, and B331) planted at low, medium, and high densities.

Defective porous urchin-like ZnO/NiO microspheres-coated solid-phase microextraction fiber for analysis of trace polychlorinated biphenyls in milk.

Owing to the high lipophilicity of polychlorinated biphenyls (PCB), they easily accumulate in dairy products. Although usually present at very low levels, they pose a serious threat to human health. Therefore, developing a sensitive and reliable method for detecting PCB in dairy products is crucial.

Type I and III collagen contents and μ-calpain autolysis as a function of dry ageing time for eight different muscles from Hanwoo cattle.

Objective: Type I and III collagen content exert contrasting influences on meat tenderness. μ-calpain autolysis has shown a correlation with beef tenderness. Thus, the study was designed to determine the changes in these proteins.

Multidentate Chelating Ligands Enable High-Performance Zinc-Bromine Flow Batteries.

Zinc bromine flow battery (ZBFB) is a promising battery technology for stationary energy storage. However, challenges specific to zinc anodes must be resolved, including zinc dendritic growth, hydrogen evolution reaction, and the occurrence of "dead zinc". Traditional additives suppress side reactions and zinc dendrite formation by altering the solvation structure of Zn and adsorbing onto the zinc surface through only a limited number of zincophilic sites, resulting in weak adsorption on zinc metal and potential inability to simultaneously optimize the solvation structure of zinc ions.

A Nonfouling Electrochemical Biosensor for Protein Analysis in Complex Body Fluids Based on Multifunctional Peptide Conjugated with PEGlyated Phospholipid.

Developing antifouling biosensors capable of performing robustly in complex human body fluids is crucial for biomarker diagnosis and health monitoring. Herein, an antifouling and highly sensitive and stable biosensor was constructed through the self-assembly of the designed conjugates composed of a multifunctional peptide (MP) and PEGylated distearoylphosphatidylethanolamine (DSPE-PEG). The self-assembly capability of the DSPE-PEG-MP was demonstrated clearly through coarse-grained molecular dynamics simulation and transmission electron microscopy, and it can be effectively self-assembled onto the electrode surface modified with gold nanoparticles.

TCD-Guided management in carotid endarterectomy: a retrospective study.

BACKGROUNDː: Stroke, primarily resulting from ischemic conditions, is the foremost cause of mortality and long-term impairment and is frequently associated with narrowing of the carotid arteries. Although carotid endarterectomy (CEA) is the treatment of choice, it carries the risk of cerebral ischemia and reduced blood flow. Transcranial Doppler (TCD) ultrasound offers a nonintrusive method to assess cerebral blood circulation during CEA, potentially enhancing surgical outcomes.

Construction of a cuproptosis‑related lncRNA signature to predict biochemical recurrence of prostate cancer.

Biochemical recurrence (BCR) is common in prostate cancer (PCa), and patients with BCR usually have a poor prognosis. Cuproptosis is a unique type of cell death, and copper homeostasis is crucial to the occurrence and development of malignancies. The present study aimed to explore the prognostic value of cuproptosis-related long non-coding RNAs (lncRNAs; CRLs) in PCa and to develop a predictive signature for forecasting BCR in patients with PCa.

Synthesizing Real-Time Ultrasound Images of Muscle Based on Biomechanical Simulation and Conditional Diffusion Network.

Quantitative muscle function analysis based on ultrasound imaging, has been used for various applications, particularly with recent development of deep learning methods. The nature of speckle noises in ultrasound images poses challenges to accurate and reliable data annotation for supervised learning algorithms. To obtain a large and reliable dataset without manual scanning and labeling, we proposed a synthesizing pipeline to provide synthetic ultrasound datasets of muscle movement with an accurate ground truth, allowing augmenting, training, and evaluating models for different tasks.

Evaluation of targeted sequencing for pathogen identification in bone and joint infections: a cohort study from China.

Purpose: Bone and joint tuberculosis (BJTB) is a distinct variant of tuberculosis in which clinical diagnosis often leads to relative misdiagnosis and missed diagnoses. This study aimed to evaluate the diagnostic accuracy of the targeted nanopore sequencing (TNPseq) assay for BJTB patients in China.

Method: The study enrolled a cohort of 163 patients with suspected BJTB.