CRISPR/Cas9-assisted gene editing reveals that EgPKS, a polyketide synthase, is required for the biosynthesis of preussomerins in Edenia gomezpompae SV2.

Edenia gomezpompae, an endophytic fungus derived from plants, produced a diverse array of preussomerins, a type of spirobisnaphthalenes featuring two spiroketal groups, which exhibited significant antibacterial, antifungal, and cytotoxic activities. Structurally, the biosynthesis of preussomerins might be related to the biosynthesis of 1,8-dihydroxynaphthalene (DHN), a precursor of DHN-melanin. However, the absence of efficient gene-editing tools for E.

Warming reduces bacterial diversity and stability in Lake Bosten.

Understanding the effects of warming on bacterial communities is essential for predicting microbial responses to climate change in aquatic ecosystems. However, the mechanisms through which warming influences bacterial diversity and stability in lake ecosystems remain poorly understood. To address this gap, we conducted a mesocosm experiment in Lake Bosten, a climate change hotspot, with three temperature scenarios (26 °C, 29 °C, and 32 °C), and investigated bacterial diversity, community composition, potential functions, and stability.

Single-cell RNA sequencing integrated with bulk RNA sequencing analysis of clock circadian regulator with prognostic and immune microenvironment in thyroid cancer.

Background: Disruption of circadian rhythm was found to be associated with immune infiltration and thyroid cancer. However, the role of clock circadian regulator (CLOCK) in the progression of thyroid cancer and its immune microenvironment remains largely unexplored. Therefore, our aim was to explore the role and potential mechanism of CLOCK in thyroid cancer.

Role of copper homeostasis and cuproptosis in heart failure pathogenesis: implications for therapeutic strategies.

Copper is an essential micronutrient involved in various physiological processes in various cell types. Consequently, dysregulation of copper homeostasis-either excessive or deficient-can lead to pathological changes, such as heart failure (HF). Recently, a new type of copper-dependent cell death known as cuproptosis has drawn increasing attention to the impact of copper dyshomeostasis on HF.

Highly Accessible Electrocatalyst with Formed Copper-Cluster Active Sites for Enhanced Nitrate-to-Ammonia Conversion.

Ammonia synthesis via nitrate electroreduction is more attractive and sustainable than the energy-extensive Haber-Bosch process and intrinsically sluggish nitrogen electroreduction. Herein, we have designed a single-site Cu catalyst on hierarchical nitrogen-doped carbon nanocage support (Cu/hNCNC) for nitrate electroreduction, which achieves an ultrahigh ammonia yield rate (YR) of 99.4 mol h g (2.

Distribution patterns and community assembly processes of bacterial communities across different sediment habitats of subsidence lakes.

Subsidence lakes, formed due to extensive underground coal mining activities, present both ecological challenges and opportunities for alternative land use practices, such as photovoltaic power generation and aquaculture. However, the ecological consequences of these anthropogenic activities on bacterial communities within subsidence lakes remain largely unexplored. To address this knowledge gap, we conducted a comprehensive investigation of bacterial communities in two typical subsidence lake districts located in Huainan, Anhui Province, China.

Functional response of two Mesocomys species on factitious host Antheraea pernyi and their biocontrol potential against the Japanese giant silkworm Caligula japonica.

Background: The Japanese giant silkworm (JGS) Caligula japonica is a significant defoliator pest in East Asia, causing severe economic losses in forest and fruit production. To establish a cost-effective biological control program against JGS, the age-stage, two-sex life table method was used to accurately assess the potential efficacy of two Mesocomys species (M. albitarsis and M.

Impact of Sintering Aid Type and Content on the Mechanical Properties of Digital Light Processing 3D-Printed SiN Ceramics.

Digital light processing (DLP) 3D-printed Si₃N₄ ceramics, known for their exceptional performance, offer distinct advantages in meeting the high-strength and complex structural demands of industries such as aerospace, semiconductors, healthcare, automotive, energy, and machinery. However, due to Si₃N₄'s strong chemical stability, low diffusion rate, low self-sintering ability, and high melting point, achieving densification under conventional sintering conditions is challenging. As a result, sintering additives are essential to promote the sintering process, lower the sintering temperature, improve densification, and enhance performance.

Z-scheme HPMoVO/g-CN heterojunction with strong photooxidative capacity for promoting efficient cleavage of C-C bond in lignin models and lignin.

The efficient photocatalytic breakage of C-C bonds has great significance for the valorization of lignin into value-added aromatic chemicals, but remains challenging owing to their demanding depolymerization conditions and high bond dissociation energies. In this study, the Z-scheme heterojunction HPMoVO/g-CN (HPA/CN) photocatalyst was elaborately developed for the selective and efficient cleaving of C-C bonds in real lignin and its β-O-4 models under mild conditions. The construction of Z-scheme heterojunction with irregular sheet micromorphology not only enhanced the charge separation and redox abilities, but also broadened the light absorption range and promoted charge-to-surface transfer in two redox components.

Adaptive evolution of extensive drug resistance and persistence in epidemic ST11 KPC-producing during antimicrobial chemotherapy.

The global rise of carbapenem-resistant , including strains producing carbapenemase (KPC) types, poses a significant public health challenge due to their resistance to critical antibiotics. Treatment options for infections caused by KPC-producing (KPC-KP) are increasingly limited, particularly as these strains develop resistance to last-line antibiotics such as ceftazidime/avibactam and colistin. This study investigates the evolution of antibiotic resistance and persistence in a series of clonally related ST11 KPC-KP strains isolated from a single patient undergoing extended antimicrobial treatment.

Heterogeneity of tumor-associated neutrophils in hepatocellular carcinoma.

Neutrophils are the most abundant cell type in human blood and play a crucial role in the immune system and development of tumors. This review begins with the generation and development of neutrophils, traces their release from the bone marrow into the bloodstream, and finally discusses their role in the hepatocellular carcinoma (HCC) microenvironment. It elaborates in detail the mechanisms by which tumor-associated neutrophils (TANs) exert antitumor or protumor effects under the influence of various mediators in the tumor microenvironment.

Epimedium polysaccharides ameliorate ulcerative colitis by inhibiting oxidative stress and regulating autophagy.

Background: Epimedium polysaccharide (EPS) is a bioactive compound derived from the traditional Chinese herb Epimedium brevicornum. The objective of this study was to investigate the protective effects of EPS on ulcerative colitis (UC) and to elucidate the underlying mechanisms involved.

Results: The findings showed that EPS treatment mitigated UC symptoms, including weight loss, anal bleeding, elevated disease activity index (DAI), and colon shortening.

Seasonal dynamics of environmental heterogeneity augment microbial interactions by regulating community structure in different trophic lakes.

Understanding how environmental heterogeneity drives microbial communities in lakes is essential for developing effective strategies to manage and restore aquatic ecosystems. However, the mechanisms by which environmental heterogeneity influences microbial community structure, network patterns, and interactions remain largely unexplored. To bridge this gap, we collected 84 water samples from four typical lakes in China (Fuxian, Tianmu, Taihu, and Xingyun) representing a range of trophic levels, across wet and dry seasons.

Incidence and outcome of biliary atresia in Shanghai, China from 2015 to 2016: a cohort study.

Demographic characteristics and clinical data of all newly diagnosed biliary atresia patients in Shanghai were collected from 1 January 2015 to 31 October 2016. The total number of live births was 377 420 during the study period, and the incidence of biliary atresia in Shanghai was 10.86 per 100 000 (95% CI 7.

Orbicular-Donor-Acceptor System in N-doped Nanographene for Highly Efficient NIR-II Photothermal Therapy.

Developing stable and efficient photothermal agents (PTAs) for the second near-infrared window (NIR-II, 1 000-1700 nm) photothermal therapy (PTT) is highly desirable but remains challenging. Herein, a facile strategy to prepare NIR-II nano-PTA based on the ionic N-doped nanographene hexapyrrolohexaazacoronene (HPHAC) is reported featuring a specific orbicular-donor-acceptor (O-D-A) structure. Oxidizing HPHAC 1 to dication 1 causes a substantial decrease in its band gap, leading to a shift in absorption from the confined UV region to a broad absorption range that reaches up to 1400 nm.

Strengthened ozone adsorption through positive electric field-induced charge migration on various TiO crystal surfaces: A mechanistic and theoretical study.

This study investigates the enhancement of ozone adsorption on diverse TiO crystal interfaces through an innovative electrochemical modulation approach. The research focuses on the effects of applied electric field strength and reaction sites on ozone interfacial adsorption energies for Ti/Anatase TiO (0 0 1) and Ti/Rutile TiO (1 1 0) interfaces. The findings reveal that positive electric fields significantly enhance ozone adsorption on both interfaces, with adsorption energies increasing by up to 18% for Ti/Anatase TiO (0 0 1) and 15% for Ti/Rutile TiO (1 1 0).

Universal Genetic Testing for Newly Diagnosed Invasive Breast Cancer.

Importance: Between 5% and 10% of breast cancer cases are associated with an inherited germline pathogenic or likely pathogenic variant (GPV) in a breast cancer susceptibility gene (BCSG), which could alter local and systemic therapy recommendations. Traditional genetic testing criteria misses a proportion of these cases.

Objective: To evaluate the prevalence and clinicopathological associations of GPVs in 2 groups of BCSGs among an ethnically diverse cohort of women with newly diagnosed breast cancer.

Solar-driven environmental fate of chlorinated parabens in natural and engineered water systems.

Parabens are classified as emerging contaminants in global waters, and the ubiquitous emergence of their high-risk chlorinated products generated from chlorine-based wastewater disinfection has attracted increasing attention. However, rather limited information is available on their photofate after discharging into surface waters, and their degradation behavior after solar-based engineering water treatment is unclear. Herein, the reactivity of four chlorinated parabens with different photochemically produced reactive intermediates was measured.

Emergence of silent NDM-1 carbapenemase gene in carbapenem-susceptible Klebsiella pneumoniae: Clinical implications and epidemiological insights.

The global dissemination of carbapenemase genes, particularly bla, poses a significant threat to public health. While research has mainly focused on strains with phenotypic resistance, the impact of silent resistance genes has been largely overlooked. This study documents the first instance of silent bla in a cluster of clonally related carbapenem-susceptible K.

Dexborneol Amplifies Pregabalin's Analgesic Effect in Mouse Models of Peripheral Nerve Injury and Incisional Pain.

Pregabalin is a medication primarily used in the treatment of neuropathic pain and anxiety disorders, owing to its gabapentinoid properties. Pregabalin monotherapy faces limitations due to its variable efficacy and dose-dependent adverse reactions. In this study, we conducted a comprehensive investigation into the potentiation of pregabalin's analgesic effects by dexborneol, a neuroprotective bicyclic monoterpenoid compound.