Increased gene dosage of RFWD2 causes autistic-like behaviors and aberrant synaptic formation and function in mice.

Autism spectrum disorder (ASD) is a neurodevelopmental disorder characterized by impaired social interactions, communication deficits and repetitive behaviors. A study of autistic human subjects has identified RFWD2 as a susceptibility gene for autism, and autistic patients have 3 copies of the RFWD2 gene. The role of RFWD2 as an E3 ligase in neuronal functions, and its contribution to the pathophysiology of ASD, remain unknown.

[Progress on the role of Kalirin-7 in exercise intervention-mediated improvement of neurodegenerative diseases].

Guanine nucleotide exchange factor Kalirin-7 (Kal-7) is a key factor in synaptic plasticity and plays an important regulatory role in the brain. Abnormal synaptic function leads to the weakening of cognitive functions such as learning and memory, accompanied by abnormal expression of Kal-7, which in turn induces a variety of neurodegenerative diseases. Exercise can upregulate the expression of Kal-7 in related brain regions to alleviate neurodegenerative diseases.

[Effects of aerobic interval training on myocardial oxidative stress and inflammation in rats with myocardial infarction and its mechanism].

The present study was to determine the effects of aerobic interval training (AIT) on the expressions of SIRT1, Nox4 and inflammatory factor in the heart of rats with myocardial infarction (MI). Male Sprague Dawley rats were randomly divided into sham-operated group (C), sedentary MI group (MI) and MI with AIT group (ME) (=10). The MI model was established by ligation the left anterior descending coronary artery.

[Research progress of the role of IGF-1 in metabolic diseases and the effect of exercise intervention].

Insulin-like growth factor-1 (IGF-1) is a peptide with a similar molecular structure to insulin. IGF-1 plays a key role in tissue growth and development, as well as cell metabolism, proliferation, differentiation and apoptosis. Liver is the main source of IGF-1, with the production of IGF-1 up to 75% of the total in the whole body, while the remaining 25% are secreted by skeletal muscles, heart, kidney, spleen and other organs.

[Effects of interval training on calcium transient and contractile function of single ventricular myocyte in myocardial infarction adult rats].

Objective: To investigate the effects of interval training on calcium transient and contractile function in ischemic ventricular myocytes of rats with myocardial infarction and their synchronization.

Methods: Twenty-four male sprague-dawley rats in three years old, were randomly divided into three groups (n=8): sham-operated group(S), sedentary MI group(MI) and MI with interval training group (ME). The MI model was established by ligation of the left anterior descending coronary artery.

[Effect of aerobic interval training on the expression of renal CD40 in a rat model with myocardial infarction and its mechanism].

Objective: To study the effects of aerobic interval training (AIT) on renal cluster of differentiation 40 (CD40) expression in rats with myocardial infarction (MI) and its possible mechanism.

Methods: Thirty-six rats were randomly divided into three groups (=12):Sham, MI and MI with AIT (ME) groups. The MI model was established by ligation of the left anterior descending coronary artery.

SIRT1 regulates lipopolysaccharide-induced CD40 expression in renal medullary collecting duct cells by suppressing the TLR4-NF-κB signaling pathway.

Aims: Recent evidence indicates that sirtuin1 (SIRT1), an NAD-dependent deacetylase, exerts a protective effect against inflammatory kidney injury by suppressing pro-inflammatory cytokines production. The co-stimulatory molecule, CD40, is expressed in a variety of inflammatory diseases in the kidney. Here, we aimed to investigate the potential effect of SIRT1 on CD40 expression induced by lipopolysaccharide (LPS) and to disclose the underlying mechanisms in renal inner medullary collecting duct (IMCD) cells.

Exercise training activates neuregulin 1/ErbB signaling and promotes cardiac repair in a rat myocardial infarction model.

Aims: Exercise training (ET) has a cardioprotective effect and can alter the molecular response to myocardial infarction (MI). The Neuregulin 1 (NRG1)/ErbB signaling plays a critical role in cardiac repair and regeneration in the failing heart. We sought to investigate whether ET following MI could activate the NRG1/ErbB signaling and promote cardiac repair and regeneration.

[Research advance on angiogenesis in ischemic heart induced by aerobic exercise and stem cell mobilization].

Aerobic exercise has specific angiogenesis effect, also in the ischemic heart. But its mechanism has not been fully clarified. Coronary microvascular angiogenesis is the precondition of heart repair after myocardial infarction (MI).

[Research advance on cardiomyocyte proliferation induced by aerobic exercise and stem cell mobilization].

Appropriate exercise is the effective way for the prevention and treatment of heart diseases. Its mechanism has not been completely elucidated, and the safe and effective exercise prescription needs to be studied systematically. Exercises give rise to secretion of various cell factors, effective stem cell mobilization, physiological hypertrophy and differentiation and proliferation of cardiomyocytes.

Aerobic exercise inhibits sympathetic nerve sprouting and restores β-adrenergic receptor balance in rats with myocardial infarction.

Background: Cardiac sympathetic nerve sprouting and the dysregulation of β-adrenergic receptor (β-AR) play a critical role in the deterioration of cardiac function after myocardial infarction (MI). Growing evidence indicates that exercise provides protection against MI. The aims of this study were to investigate whether aerobic exercise following MI could inhibit sympathetic nerve sprouting and restore the balance of β3-AR/β1-AR.

Exercise training attenuates hypertension and cardiac hypertrophy by modulating neurotransmitters and cytokines in hypothalamic paraventricular nucleus.

Aims: Regular exercise as an effective non-pharmacological antihypertensive therapy is beneficial for prevention and control of hypertension, but the central mechanisms are unclear. In this study, we hypothesized that chronic exercise training (ExT) delays the progression of hypertension and attenuates cardiac hypertrophy by up-regulating anti-inflammatory cytokines, reducing pro-inflammatory cytokines (PICs) and restoring the neurotransmitters balance in the hypothalamic paraventricular nucleus (PVN) in young spontaneously hypertensive rats (SHR). In addition, we also investigated the involvement of nuclear factor-κB (NF-κB) p65 and NAD(P)H oxidase in exercise-induced effects.

[Exercise intervention in skeletal muscle endocrine function].

As a new secretory organ skeletal muscle, which could secrete a variety of biological active substances, plays an important role in biological function and clinical medicine, and has important research value and application scenarios in the field of sports medicine. Different mode and intensity of exercise would give different influences on skeletal muscle endocrine function. Exercise intervention could improve the chronic disease, such as metabolic disease (obesity, diabetes) and muscle atrophy by changing endocrine function of the skeletal muscle.

Protective effects of apricot kernel oil on myocardium against ischemia-reperfusion injury in rats.

The present study was undertaken to evaluate the potential cardioprotective effects of apricot kernel oil (AO) on the myocardial ischemia-reperfusion (IR) of rat model in vivo. The rats were divided into five groups: sham-operated, IR, low dose AO-treated IR (LD-AO+IR), medium dose AO-treated IR (MD-AO+IR) and high dose AO-treated IR (HD-AO+IR). All rats were provided with food and water ad libitum.

[Synthesis, solubility, lipids-lowering and liver-protection activities of sulfonated formononetin].

A water-soluble compound, sodium formononetin-3'-sulfonate with good lipid-lowering and liver-protection activities was synthesized. It was synthesized by sulfonation reaction, and its structure was characterized by IR, NMR and elemental analyses. The solubility of sodium formononetin-3'-sulfonate in water and n-octanol/water partition coefficient were determined by UV spectrophotometry.

[Effects of exercises training on the rats aorta stress and expression of NF-kappaB and c-Fos].

Aim: To investigate the effect of different intensity exercise training on the rat aorta stress and expression of NF-kappaB and c-Fos.

Methods: We used treadmill exercise to establish the rat models of aerobic exercise and fatigue exercise, applied immunohistochemistry of SABC to measure the effect of different intensity exercise training on expression of NF-kappaB and c-Fos in the VEC and VSMC of rat aorta.

Results: The change of aorta blood pressure, compared with the control group, which had significant increase in both aerobic exercise and fatigue exercise (P < 0.

Aging-associated insulin resistance predisposes to hypertension and its reversal by exercise: the role of vascular vasorelaxation to insulin.

Aging is an independent risk factor for hypertension, and hypertension and insulin resistance commonly coexist in the elderly. This study was designed to examine the effects of aging-related insulin resistance on blood pressure (BP) and its underlying mechanisms, with specific focus on the role of exercise in reversing hypertensive response. Adult (6-month-old) and aging (24-month-old) male Sprague-Dawley rats were subjected to a 10 weeks free-of-loading swim training (60 min/day, 5 days/week).

[Effect of aerobic exercise on living myocardial nuclear calcium transport in rats].

Aim: To investigate the biological mechanism of the dynamic changes of living myocardial nuclear calcium which was isolated from aerobic exercised rats.

Methods: Acutely isolated myocardial cells were incubated with STDBT and Fluo-3Ca2+ fluorescent indicator. The changes of the fluorescence signal of free calcium were measured by laser scanning confocal microscope (LSCM).

Cardiac muscle sarcolemma chromatographic stationary phase and its potential application in drug screening.

A new bioactive packing material for liquid chromatography, sarcolemma chromatography stationary phase (SCSP), is presented. Its surface characteristics are investigated, and it is found that the acceptors embedded in sarcolemma remained bioactive for more than a week. The retention behavior of antagonists and activators related to cardiac muscle sarcolemma on the SCSP chromatographic column shows the screening function of the SCSP column, and the retention behavior of the active components in the aether extract from the Chinese herb Ligusticum chuanxiong Hort.

[Study on the interactions between Ligusticum chuanxiong extract and cardiac muscle membrane receptors by CMSP chromatography].

Objective: To study the interactions between Ligusticum chuanxiong Hort extract and cardiac muscle membrane receptors.

Method: The cell membrane of rabbit cardiac muscle was fixed on silicon to make cell membrane stationary phase (CMSP), and then the interactions were studied by comparing the retention characteristics of the extracts from different solvents with those of the antagonists or activators corresponding to known receptors in cardiac muscle membrane, and by competition effect on the retention characteristics of extracts when adding the antagonists or activators into the mobile phase.

Result: Water extract and ethanol extract both had retentions on CMSP; the retention characteristics of water extract could be affected when water extract was in competition with the antagonists for alpha receptor, and could not be affected when with the activator beta1 receptor.

[Screening models and techniques for medicine and its application in the studies on the active components of Chinese medicine].

The development of modern biologic techniques have provided new techniques and approaches for the modern studies on active components of Chinese medicine. This article is a review of four kinds of screening models and corresponding techniques for medicine and their applications in the studies on the active components of Chinese medicine. The four aspects comprise the whole animal models, receptor models and molecular biochromatography, cell models and cell membrane chromatography, and gene chip techniques.