Techniques and applications in 3D bioprinting with chitosan bio-inks for drug delivery: A review.

Three-dimensional bioprinting leverages computer-aided design to construct tissues and organs with specialized bioinks. A notable biomaterial for this purpose is chitosan, a natural polysaccharide sourced from crustacean exoskeletons. Chitosan's biocompatibility, biodegradability, non-toxicity, and ability to promote cell adhesion and proliferation make it an excellent component for bioinks.

Artificial intelligence-based diagnosis of Alzheimer's disease with brain MRI images.

Alzheimer's disease, a primary neurodegenerative condition, predominantly impacts the elderly and pre-elderly population. This progressive neurological disorder is characterized by an array of symptoms including memory loss, cognitive decline, and various physiological and psychological disturbances, significantly compromising the quality of life of patients and their caregivers. Recent advancements in Magnetic Resonance Imaging (MRI) technology have catalyzed research in AI-enhanced diagnostics for Alzheimer's disease, fostering optimism for early detection and timely interventions.

Endoplasmic reticulum stress-activated glycogen synthase kinase 3β aggravates liver inflammation and hepatotoxicity in mice with acute liver failure.

Endoplasmic reticulum stress (ER stress) has been increasingly recognized as an important mechanism in various liver diseases. However, its intrinsic physiological role in acute liver failure (ALF) remains largely undetermined. This study aimed to examine how ER stress orchestrates glycogen synthase kinase 3β (GSK3β) and inflammation to affect ALF.

Screening for serum biomarkers in patients with chronic hepatitis B with hepatitis B surface antigen seroclearance, following pegylated interferon alpha therapy.

Chronic hepatitis B (CHB) is one of the most common infectious disease worldwide and a leading cause of death. Hepatitis B surface antigen (HBsAg) has previously been proven to be a steady biomarker that may be used to predict clinical outcomes. The amount of circulating HBsAg has been reported to reflect the number of infected hepatocytes.

[Effect of grape procyanidins on expressions of Caspase-3 and Bax in ethanol-induced liver injury in mice].

Objective: To study the effect of grape procyanidins (GPC) on the expressions of Caspase-3 and Bax in the liver of mice with ethanol-induced liver injury.

Method: The mice were orally given at the different doses of GPC (50,100 and 200 mg/kg) and 4 g/kg ethanol, and killed after 4 weeks. The proliferation activity of the hepatic cells was determined by MTT assay,the levels of Caspase-3 and Bax protein expression in the cells were measured by immunohistochemistry method.

[Phenotypic variation in Actinobacillus actinomycetemcomitans].

Objective: To investigate the colony variation in Actinobacillus actinomycetemcomitans (Aa) from rough to smooth and recognize its different morphology during laboratory translations.

Methods: Primary strains isolated from subgingival plaque of two juvenile periodontitis patients were repeatedly subcultured on agar plates and broth; for broth culture, every generation was translated in broth and on solid medium separately to observe the corresponding morphologies of Aa grow in broth.

Results: Three smooth strains of Aa from the broth culture were obtained.