[Using purified conjugated bilirubin as a calibrator for bilirubin measurement].

Objectives: To improve the accuracy and precision of the determination of bilirubin, especially direct bilirubin (DB), and the standardization of that as well.

Methods: Purified conjugated bilirubin (Bc) and ditaurobilirubin(DTB) and their diazo products were subjected to absorption spectrum analysis. The diazo reaction characters of their calibration solutions were compared by the method of Doumas J-G(TB & DB).