Publications by authors named "Zhejian Yu"
Evaluation of T Cell Receptor Construction Methods from scRNA-Seq Data.
Genomics Proteomics Bioinformatics
December 2024
Article Synopsis
- * A benchmark study was conducted using experimental single-cell immune profiling datasets, highlighting that the methods TRUST4 and MiXCR provided superior performance in TCR reconstruction, while DeRR also showed notable accuracy.
- * The introduction of the YASIM-scTCR simulator allows for the generation of diverse TCR-derived sequences, but the study found that sequencing depth significantly affects the success of TCR construction from scRNA-seq data.
The advancement of Long-Read Sequencing (LRS) techniques has significantly increased the length of sequencing to several kilobases, thereby facilitating the identification of alternative splicing events and isoform expressions. Recently, numerous computational tools for isoform detection using long-read sequencing data have been developed. Nevertheless, there remains a deficiency in comparative studies that systemically evaluate the performance of these tools, which are implemented with different algorithms, under various simulations that encompass potential influencing factors.
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