ALKBH1: emerging biomarker and therapeutic target for cancer treatment.

As neoplastic cells proliferate, disseminate, and infiltrate, they undergo substantial alterations in their epigenetic configuration. Among the pivotal enzymes implicated in this phenomenon is the AlkB family of demethylases, notably AlkB homolog 1 (ALKBH1), which demonstrates conspicuous upregulation across various malignancies. The heightened expression of ALKBH1 renders it a compelling candidate for the development of multifaceted anticancer modalities.

Risk factors for complications of Mycoplasma pneumoniae pneumonia in hospitalized children in China: a systematic review and meta-analysis.

Background: Mycoplasma pneumoniae pneumonia (MPP) is a common respiratory disease in children. Some patients may develop long-term respiratory issues such as chronic cough and wheezing due to complications. These complications increase the health burden for children with MPP, highlighting the need to understand their risk factors in order to improve disease prognosis.

The transcription factor OsNAC25 regulates potassium homeostasis in rice.

Over-application of potassium (K) fertilizer in fields has a negative impact on the environment. Developing rice varieties with high KUE will reduce fertilizer for sustainable agriculture. However, the genetic basis of KUE in a more diverse and inclusive population remains largely unexplored.

[Application and prospects of infrared thermography in rheumatic diseases].

Infrared thermography is an advanced technology that utilizes infrared detectors to sense the infrared thermal radiation emitted from the human body' s surface, converting it into electrical signals. These signals are then processed by computers to generate planar temperature color images, providing an intuitive display of the body surface temperature distribution. Rheumatic diseases, as prevalent conditions that significantly impact the quality of life of millions of people worldwide, pose significant challenges in diagnosis and assessment.

Impact of Cooking Duration on Calcium Oxalate Needle-like Crystals in Plants: A Case Study of Vegetable Taro Flowers in Yunnan.

As a popular vegetable in Yunnan Province, China, taro flowers are delicious but contain substances that can cause numbing and mucous membrane damage. Prolonged high-temperature cooking is used by locals to mitigate these effects, though its mechanisms were previously unexplored. This study confirms the presence of needle-like calcium oxalate crystals in taro flowers and shows that prolonged steaming reduces their quantity, size, and sharpness, making them safer to eat.

Higher intake energy, protein, and polyunsaturated fatty acids at dinner versus breakfast increase the risk of hyperhomocysteinemia among adults in the USA.

Purpose: This study analyzed the relation of energy and macronutrient intake at dinner versus breakfast with the risk of hyperhomocysteinemia (Hhcy).

Methods: Up to 12,474 adults, in which 1,387 with Hhcy, completed a questionnaire about energy and macronutrient intake in the National Health and Nutrition Examination. The differences (Δ) in that between dinner and breakfast (Δ = dinner - breakfast) were categorized into quartiles.

Analyzing the Effect of Resveratrol on Pharmacokinetics of Antituberculosis Drug Bedaquiline in Rats by a Novel UPLC-MS/MS Approach.

Bedaquiline (BDQ), a diarylquinoline compound, is an inhibitor of mycobacterial ATP synthase, specifically with FDA approval as a treatment for multidrug-resistant tuberculosis (MDR-TB). M2 is the main metabolite of BDQ and is active against tuberculosis. The objective of this study was to establish and validate a sensitive and convenient ultraperformance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) approach to concurrently quantify BDQ and M2 in rat plasma and to examine whether resveratrol, a CYP3A4 inhibitor, could influence the pharmacokinetics of BDQ and M2 in rats.

Polystyrene microplastics disrupt adrenal steroid synthesis in male mice via mitochondrial dysfunction.

Microplastics have gained significant social attention, as they can enter our bodies through food and drinking water. The adrenal gland is essential for the maintenance of metabolic homeostasis and stress responses. Nevertheless, the effects of microplastics on the steroid synthesis in the adrenal cortex was still unclear.

Ciliary length regulation by intraflagellar transport in zebrafish.

How cells regulate the size of their organelles remains a fundamental question in cell biology. Cilia, with their simple structure and surface localization, provide an ideal model for investigating organelle size control. However, most studies on cilia length regulation are primarily performed on several single-celled organisms.

Melatonin inhibits the activation of microglia and cough sensitivity of guinea pigs exposed to PM2.5.

Objective: The study aimed to examine the impact of melatonin on mitigating brain inflammation and cough sensitivity resulting from exposure to particulate matter 2.5 (PM2.5).

Huashi baidu granule alleviates inflammation and lung edema by suppressing the NLRP3/caspase-1/GSDMD-N pathway and promoting fluid clearance in a porcine reproductive and respiratory syndrome (PRRS) model.

Ethnopharmacological Relevance: Huashi Baidu Granule (HSBDG), a traditional Chinese medicine (TCM), is used for treating coronavirus disease 2019 (COVID-19). Porcine reproductive and respiratory syndrome (PRRS) is considered the "COVID-19" for swine. According to the TCM theory, "dampness" is the main pathogenic factor in COVID-19 and PRRS, and "Huashi" means that this formula is good at removing "dampness".

Global and regional genetic association analysis of ulcerative colitis and type 2 diabetes mellitus and causal validation analysis of two-sample two-way Mendelian randomization.

Background: Clinical co-occurrence of UC (Ulcerative Colitis) and T2DM (Type 2 Diabetes Mellitus) is observed. The aim of this study is to investigate the potential causal relationship between Ulcerative Colitis (UC) and Type 2 Diabetes Mellitus (T2DM) using LDSC and LAVA analysis, followed by genetic verification through TSMR, providing insights for clinical prevention and treatment.

Methods: Genetic loci closely related to T2DM were extracted as instrumental variables from the GWAS database, with UC as the outcome variable, involving European populations.

Absorbable gelatin sponge as an intrascleral buckle in rabbits: a pilot study.

Purpose: This study aimed to evaluate the efficacy and safety of using an absorbable gelatin sponge as an intrascleral buckle in rabbit eyes; it also monitored degradation of the gelatin sponge over time.

Methods: Ten New Zealand white rabbits underwent surgery to implant an absorbable gelatin sponge as an intrascleral buckle. Weekly ophthalmic examinations were conducted before and after the procedure until the implant was fully absorbed.

Remote C-H bond cooperation strategy enabled silver catalyzed borrowing hydrogen reactions.

Metal-ligand cooperation (MLC) is an essential strategy in transition metal catalysis. Traditional NH-based and OH-based MLC catalysts, as well as the later developed (de)aromatization strategy, have been widely applied in atom-economic borrowing hydrogen/hydrogen auto-transfer (BH/HA) reactions. However, these conventional MLC approaches are challenging for low-coordination and low-activity coinage metal complexes, arising from the instability during (de)protonation on the coordination atom, the constraint in linear coordination, and possible poisoning due to extra functional sites.

Clinical use of Gafchromic EBT4 film for in vivo dosimetry for total body irradiation.

Purpose: In vivo dosimetry is a common requirement to validate dose accuracy/uniformity in total body irradiation (TBI). Several detectors can be used for in vivo dosimetry, including thermoluminescent dosimeters (TLDs), diodes, ion chambers, optically stimulated luminescent dosimeters (OSLDs), and film. TLDs are well established for use in vivo but required expertise and clinical system availability may make them impractical for multifractionated TBI.

Development of Polyclonal Antibodies for the Preliminary Characterization of GPATCH1, a Novel Splicing Factor Associated with Human Osteoporosis.

Specific antibodies, which can be used in various experiments, are critical tools for unraveling genes' function, but many commercial antibodies are not tested for these properties. GPATCH1 is a novel G-patch family protein. Genome-wide association studies (GWAS) revealed it as a gene associated with human osteoporosis, and yeast-based research suggested it may be a splicing factor; however, its molecular mechanism remains a mystery.

Generalized Gaussian Distribution Improved Permutation Entropy: A New Measure for Complex Time Series Analysis.

To enhance the performance of entropy algorithms in analyzing complex time series, generalized Gaussian distribution improved permutation entropy (GGDIPE) and its multiscale variant (MGGDIPE) are proposed in this paper. First, the generalized Gaussian distribution cumulative distribution function is employed for data normalization to enhance the algorithm's applicability across time series with diverse distributions. The algorithm further processes the normalized data using improved permutation entropy, which maintains both the absolute magnitude and temporal correlations of the signals, overcoming the equal value issue found in traditional permutation entropy (PE).

Shared genetic architecture of psychiatric disorders and hemorrhoidal disease: a large-scale genome-wide cross-trait analysis.

Background: The genetic association between psychiatric disorders and hemorrhoidal disease (HEM) is still not well known. The work aims to investigate their comorbidity at a genetic level.

Methods: Utilizing recent large-scale genome-wide association studies (GWAS), we investigated the genetic overlap at the single nucleotide polymorphism (SNP), gene, and molecular level between depression and HEM, bipolar disorder (BD) and HEM, neuroticism and HEM, as well as schizophrenia (SCZ) and HEM.

Structural basis for Retriever-SNX17 assembly and endosomal sorting.

During endosomal recycling, Sorting Nexin 17 (SNX17) facilitates the transport of numerous membrane cargo proteins by tethering them to the Retriever complex. Despite its importance, the mechanisms underlying this interaction have remained elusive. Here, we provide biochemical, structural, cellular, and proteomic analyses of the SNX17-Retriever interaction.

Establishment and characterization of the first immortalized vocal cord leukoplakia epithelial cell line.

The importance of vocal cord leukoplakia (VCL) in the etiology and progression of laryngeal carcinoma has gained increasing recognition. However, research into the mechanisms of laryngeal precancerous lesions such as VCL, has been hampered by the small size of VCL epithelial cells and their limited culture lifespan. In this study, we enhanced the primary culture protocol for VCL epithelial cells and introduced simian virus 40 Large T to establish an immortalized cell line, designated hVCL-MSDEP01.

All-fiber quantum relaxometry for biochemical sensing based on diamond NV centers.

The longitudinal relaxation time (termed as T) of nitrogen-vacancy (NV) centers in nanodiamonds can be affected by surface electric or magnetic noise, which has been exploited to develop cutting-edge quantum relaxometry for biochemical sensing. In this work, a tiny all-fiber quantum probe based on longitudinal relaxometry was developed by chemically-anchoring nanodiamonds on the surface of a cone fiber tip. The dependences of T on surface electric and magnetic noise were discussed in theories first and then experimentally demonstrated in varied pH and Gd concentration solutions, respectively.