[Quantitative characterization of antagonistic activity of lactobacilli].

In this work the method of serial dilutions of lactobacilli in two-layer agar was used. On the agar surface bacterial or fungal cultures were applied at different time intervals. A special quantitative characteristic was introduced.

[Escherichia coli reactions to the action of the cationic proteins of blood leukocytes].

The antibacterial effect of cation proteins (CP) of swine leukocytes with respect to Escherichia coli strain 17 has been demonstrated in vitro. The composition and properties of these CP have been studied; as a result, a comparatively high content of basic and dicarbonic amino acids and the presence of protease activity have been established. The release of the components of the metabolic fund by E.

[The effect of the cationic proteins of human blood cells on the growth of Escherichia coli].

The antibacterial effect of cationic proteins (CP) on donor leukocytes and thrombocytes with respect to the growth of E. coli has been demonstrated in vitro, the maximum recorded inhibition being caused by the action of leukocytic CP. Differences in the inhibitory action may be linked with the presence of anomalies in the amino acid composition of leukocytic CP and thrombocytic CP, manifested by the deterioration of the basic properties of the latter, as well as by the fractional composition whose characteristic features for thrombocytic CP are the appearance of high-molecular components and a decrease in the proportion of low-molecular fractions.

[Succinate dehydrogenase activity of Escherichia coli cells after heat stress and during the reparative process].

The resting cells of different E. coli cells remained viable after their heating at 48 degrees C for 30 min. The activity of their succinate dehydrogenase (SDH) (EC 1.

[Exogenous orthophosphate regulation of the phosphohydrolase activities of Pseudomonas aeruginosa and Pseudomonas maltophilia].

The activity of several phosphohydrolases, viz. alkaline phosphatase (EC 3.1.

[Relationship of Pseudomonas maltophilia alkaline phosphatase to its membranes].

The localization of alkaline phosphomonoesterase (EC 3.1.3.

[Phosphohydrolase activity of Pseudomonas maltophilia].

The activity of phosphohydrolase (acid, alkaline, pyrophosphatase) was studied in two strains of Pseudomonas maltophilia: VCM-B-591 and SSE-B-715. The activity of these enzyme systems in these strains was found to be much higher than in other Pseudomonas species, viz. Ps.

[Effect of histones on a change in the enzymatic activity of Escherichia coli and Staphylococcus aureus cells].

Histones have been found to interfere with the permeability of the cellular membranes of Escherichia coli and Staphylococcus aureus, this resulting in the liberation of 14C-amino acids, the UV-absorbing endogenous material, and the products of RNA degradation. Histones, particularly arginine-rich fractions, stimulated the RNAase activity of the bacteria. Histones inhibited the phosphatase activity of E.

[Effect of thymus histones on the RNAase, ATPase and phosphatase activity of staphylococcal cells].

It was shown that the representatives of different strains of Staphylococcus genus were characterized by a differential histone sensitivity. The membrane permeability increased in case of the bacterial contact with histones, and the degrading of the sum total cell RNA occurred in the contact of bacteria with histones. The RNA-ase, phosphatase, and the ATP-ase activity increased in the cells after their treatment with histones.

[Differential sensitivity of bacteria to histones].

The antimicrobial action of polycation proteins from the nuclei--histons with a high content of lysine (f1) and arginine (f3)--on Pseudomonas bacteria was studied. The sensitivity of various species of the same genus, and various strains of the same species, to histons was differential. The differences do not depend on the ability of the cells to liberate into a surrounding medium substances inactivating histons, and on the rate of histon sorption on the cells.

[Effect of histones on cell permeability, protein and RNA synthesis and RNA stability in Aerobacter cloaceae].

The histones of calf thymus were found to change the permeability of the cells of Aerobacter cloaceae, without producing any noticeable effect on their viability. Concomitant depolymerization of the cellular RNA indicates destruction of the ribosomes. The increased synthesis of RNA in interpreted as a manifestation of reparatory processes.