Nephroprotective Effect of Leu-Ile-Lys Tripeptide in Experimental Diabetes Mellitus.

We studied the effect of tripeptide Leu-Ile-Lys on kidney function in rats with experimental diabetes mellitus modeled by single intraperitoneal administration of streptozotocin (65 mg/kg). The tripeptide was intragastrically administrated in a dose of 11.5 mg/kg from week 5 to week 8 of the pathological process.

Experience of Using Tripeptide Leu-Ile-Lys for Experimental Therapy of Chronic 16-Week Oxalate Nephrolithiasis in Rats.

We studied the effect of tripeptide Leu-Ile-Lys on the course of chronic 16-week oxalate nephrolithiasis in rats modeled by administration of 1% ethylene glycol solution in drinking water for 16 weeks. The tripeptide Leu-Ile-Lys obtained by chemical synthesis (sample purity ≥98%) was administered intragastrically through a probe in a dose of 11.5 mg/kg in 1 ml saline.

Effect of Tripeptide Leu-Ile-Lys on the Course of Experimental Nephrolithiasis.

We studied the effect of Leu-Ile-Lys tripeptide on the course of experimental oxalate nephrolithiasis modeled in rats by administration of 1% ethylene glycol solution instead of drinking water for 6 weeks. The Leu-Ile-Lys tripeptide obtained by chemical synthesis (purity ≥98%) was administered through a gastric tube (11.5 mg/kg in 1 ml saline).

[The features of the pathologic changes in chronic oxalate stone disease after 16 week of experimental phase].

Aim: to study the pathologic features of chronic oxalate stone disease.

Materials And Methods: a model of the experimental oxalate stone disease was done in rats that drank the 1% ethylene glycol solution for 16 weeks. The pathologic analysis of the rats kidneys was done.

Morphological Evaluation of the Influence of the Peptide Complex from Tissue of Porcine Kidneys on the Experimental Urolithiasis.

We performed morphological analysis of the effect of the peptide complex from porcine kidneys on the course of experimental urolithiasis modeled in rats by treatment with 1% ethylene glycol solution (in drinking water) for 6 weeks. The peptide complex obtained by acetic acid extraction was administered in a dose of 15 mg. Administration of the peptide complex to animals with experimental kidney stone disease leads to 100% destruction of large and medium stones to the "dust" granularity.

[Formation of flavor of dry champignons (Agaricus bisporus)].

The composition of aroma compounds of dry champignons (Agaricus bisporus L.) were identified using capillary gas chromatography and chromatography-mass spectrometry. In total, 56 compounds were identified.

[The composition of volatile components of dry cepe and oyster mushroom].

The composition of aroma compounds in dry cepe mushroom (Boletis edulis Fr.) and oyster mushroom (Pleurotus ostreatus Fr.) was studied using capillary gas chromatography and chromatography-mass spectrometry.

[The composition of volatile components of cepe (Boletus edulis) and oyster mushrooms (Pleurotus ostreatus)].

The composition of aroma compounds in cooked and canned cepe (Boletus edulis) and in cooked oyster mushrooms (Pleurotus ostreatus) is studied using capillary gas chromatography and chromatography-mass spectrometry. It is found that unsaturated alcohols and ketones containing eight atoms of carbon determine the aroma of raw mushrooms and take part in the formation of the aroma of cooked mushrooms as well. The content of these compounds was the highest in canned cepes.

[Electron microscopic study of the cell arrangement in bacterial colonies].

The microstructure of colonies of Bacillus brevis var. G.--B R-, S-, P+-, P- -variants and Caryophanon latum and C.

[Diurnal changes in the lysosomal enzyme activity of the liver of mice and rats].

Lysosomal enzymes acid phosphatase, acid DNAase, acid RNAase cathepsin D,beta-galactosidase beta-glucuronidase were studied in mice and rat liver tissue within 3 days beginning from 4 o'clock a.m. at 4 hrs intervals.

[Effect of choriogonine on the enzymatic activity of lysosomes in the cirrhotic liver in the rat].

Liver tissue cirrhosis, developed in rats after long-term administration of CCl4, led to distinct increase in activities of acid phosphatase, acid DNAase, cathepsin D, beta-galactosidase and beta-glucosidase. When the treatment with the hepatotropic toxins was stopped activity of lysosomal enzymes decreased slightly but was maintained at the high level. Within a day after a single administration of choriogonine distinct decrease as compared with the controls in activity of all the acid hydrolases studied was found.

[Relationship between morological variability of Bacillus subtilis R-623 and biosynthesis of hydrolytic enzymes].

By synthetic sorbent chromatography the influence of Bacillus subtilis R-623 morphology on the qualitative and quantitative composition of alpha-amylase and proteases was studied. It was found that morphological variants of natural variability of Bac. subtilis R-623, alpha-amylase producer, differed in their cultural, morphological and physiological properties as well as in the amount of hydrolytic enzymes synthesized per unit of the cultural medium.

Development of higher fungi under weightlessness.

The development of Polyporus brumalis basidomycete cultures, both in the dark and in the light, was studied in the 17 day experiment on the orbital station Salyut 5 and in the 20 day experiment on the orbital station Salyut 6. In the first experiment the test tube cultures were exposed to weightlessness beginning from the stage of the fruiting body primordium. In the second experiment the culture was exposed in large containers at the stage of mycelium growth.

Biological investigations on the orbital station "Salyut-5".

Genetic and embryological investigations were continued on the orbital station Salyut-5. The effect of space flight factors on dry Crepis capillaris (L) Wallr seeds and seedlings, dry Arabidopsis thaliana (L) Heynh seeds, Polyporus brumalis Pers. ex.

[Physiological and biochemical characteristics of Bacillus subtilis variants that form amylase].

Physiologo-biochemical properties of four Bacillus subtilis variants R-623 (R, S, P and M) were studied; the variants were isolated by inoculation of the cell population. All the four variants were shown to belong to the studied species and to possess common and specific properties. They differed in 11 characters: the size of cells and spores, the ability to hydrolyse starch, the mode of growth on specific media, the spectrum of antibiotic action, etc.

[Cytomorphology of the lipid inclusions of Caryophanon during its growth on an agarized medium].

The cytomorphology of lipid inclusions of Caryophanon latum and Caryophanon tenue was studied in the course of their growth on a solid medium. The paper presents a detailed scheme and microphotographs showing cytomorphological changes in the inclusions of the cultures under study. Staining of the preparations with the lipophilic dye Sudan Black B revealed two types of sudanophilic granules which varied in their location within trichomes, the time at which they appeared in bacteria, and their behaviour toward solvents.

Effects of weightlessness, space orientation and light on geotropism and the formation of fruit bodies in higher fungi.

The effect of flight factors on the growth and development of the fruit bodies of the higher fungus Polyporus brumalis is investigated under the conditions of an experiment on the earth satellite Cosmos 690. Fungi were grown on the earth under conditions of simulated flight and transit; fungi were also grown in the laboratory at a temperature similar to that in flight, at various orientations and under various illuminations. Polyporus brumalis grows well on wort; it forms fruit bodies on long pedicles (up to 25 cm) and possesses a strongly marked negative geotropism.

[Some properties of 1 P+f bacteriophage for Bacillus brevis var. G.-B and its nucleic acid].

The 1 P+f phage, a virulent mutant of the moderate P+ phage for Bac. brevis var. G.

[Effect of gramicidin C on the formation and germination of Bacillus brevis var. GB (P+-variant) spores].

The effect of gramicidin C added to the medium at various periods of cultivation in concentrations of 20, 40 and 100 gamma/ml on sporulation of P+-variant of Bac. brevis var. GB was studied.

[Effect of different factors on the germination of spores in S and P- variants of Bacillus brevis].

The effect of temperature, duration of heating and the presence of L-alanine and L-glutamine in the medium on the spore germination was studied with the S and P- variants of Bacillus brevis which did not contain gramicidin S and with the R and P+ varants obtained on a defined medium with beta-phenyl-beta-alanine, an inhibitor of the biosynthesis of gramicidin S. The experiments were carried out according to the scheme of complete factor experiment. Germination of the spores was found upon their incubation in a defined medium with L-alanine within two hours after their preliminary heating at 80 degrees C during 45 minutes (S variant), at 60 degrees C during 45 minutes (R variant+trace amounts of gramicidin S), at 80 degrees C during 15 minutes (P+ variant/trace amounts of gramicidin S).