Impacts of manufacture processes and geographical regions on the microbial profile of traditional Chinese cheeses.

The microbiota of cheese plays a critical role in determining its organoleptic and other physicochemical properties. Thus, assessing the composition of the cheese microbiota community would help promote the growth of desirable taxa and ultimately to optimize flavor, quality and safety. Here we measured microbial diversity, microbiota composition, short-chain fatty acids, and free amino acids in two traditional cheese-making strategies, Rushan and Rubing, processed in parallel from Lijiang, Eryuan, and Dengchuan of Yunnan province, China.

Co-immobilization of multi-enzyme on reversibly soluble polymers in cascade catalysis for the one-pot conversion of gluconic acid from corn straw.

The difficulties in the process of cellulose cascade conversion based on immobilization technology lies in the recycling enzymes from rich solid-containing straw hydrolysate and the incompatibility of conventional immobilization with this process. In this study, three types of enzyme (cellulase, glucose oxidase and catalase) were successfully immobilized on a reversible soluble Eudragit L-100. Through the determination of the preparation conditions, enzymatic properties and catalytic conditions, the co-immobilized enzyme was applied to the catalytic reaction of one-pot conversion of corn straw to gluconic acid.

Synergistic effect of thermostable β-glucosidase TN0602 and cellulase on cellulose hydrolysis.

Thermophilic enzymes have many potential benefits in industrial production with increased flexibility related to process configurations. A thermostable β-glucosidase from Thermotoga naphthophila RUK-10 was found to possess catalytic activity for cellobiose hydrolysis with a high potential for application in biomass conversion. The aggregation of cellobiose often has an inhibitory effect on cellobiohydrolases and endoglucanases during cellulose hydrolysis.