Publications by authors named "Zhaoxiu Zhang"
[New insight into the mechanism of p53 inducing leukemia cell apoptosis].
Zhonghua Xue Ye Xue Za Zhi
December 2003
Objective: To investigate the expression changes of intrinsic cytokines TGF-beta(1) and TNF-alpha, telomerase activity and bcl-2 during ongoing apoptosis of HL-60 and K562 cells induced by p53.
Methods: pN53cG (Val135), a temperature sensitive p53 mutant, which behaved like wild type p53 (wt-p53) at 32.5 degrees C, were introduced into p53-null HL-60 and K562 cells respectively by lipofectin.
[Real-time quantitative RT-PCR for the detection of bcl-2 mRNA expression].
Zhonghua Yi Xue Yi Chuan Xue Za Zhi
October 2002
Objective: To establish a real-time quantitative RT-PCR method for the detection of expression of bcl-2 mRNA.
Methods: The vector containing bcl-2 gene as standard template was constructed with T-A cloning technique. The fluorogenic probe (i.