Ozone treatment regulated the anti-exercise fatigue effect of fresh-cut pitaya polyphenol extracts.

The anti-exercise fatigue effect of plant polyphenols is closely related to the content, composition, and extractability of phenolic substances. According to our previous findings, ozone treatment significantly affected the biological effects of fresh-cut pitaya polyphenols. Therefore, this study used C57BL/6 J mice to explore the regulatory mechanism of ozone treatment on the anti-exercise fatigue effect of fresh-cut pitaya polyphenol extract.

Research progress on the effect of medicine and food homology resources for sleep improvement.

Insomnia can have a negative impact on people's life or even cause mental or physical diseases. In China, the usage of medicine food homology herbal resources to treat insomnia has a long history. This review, which is based on the theory of traditional Chinese medicine (TCM), summarizes the research progress of medicine and food homology (MFH) resources in treating insomnia.

Annonaceous acetogenins mimic AA005 targets mitochondrial trifunctional enzyme alpha subunit to treat obesity in male mice.

Obesity and related diseases pose a major health risk, yet current anti-obesity drugs inadequately addressing clinical needs. Here we show AA005, an annonaceous acetogenin mimic, resists obesity induced by high-fat diets and leptin mutations at non-toxic doses, with the alpha subunit of the mitochondrial trifunctional protein (HADHA) as a target identified through proteomics and in vitro validation. Pharmacokinetic analysis shows AA005 enriches in adipose tissue, prompting the creation of adipose-specific Hadha-deficient mice.

WDR68 stimulates cellular proliferation via activating ribosome biogenesis in 293T cells.

WDR68, a conserved WD40 repeat-containing protein, interacts with E1A and is involved in the E1A-induced cell proliferation and oncogenic transformation, but the intrinsic molecular mechanisms of this process remain to be elucidated. Here, we demonstrate that WDR68 promotes the proliferation of 293T cells by interacting with a series of ribosome biogenesis-regulating proteins. Gene Set Enrichment Analysis (GSEA) of RNA-seq data also revealed that the ribosome biogenesis-associated gene signatures could be the most significantly enriched in the WDR68 expression groups.

Transcriptome analysis reveals ozone treatment maintains ascorbic acid content in fresh-cut kiwifruit by regulating phytohormone signalling pathways.

Ascorbic acid (AsA) is an indicator of the nutritional value of freshly cut kiwifruit during storage at 4℃, and its degradation can be inhibited after ozone treatment (1 mg/L, 10 min). The aim of this study was to elucidate the regulatory mechanism affecting AsA metabolism in fresh-cut kiwifruit after ozone treatment. In this study, ozone treatment not only prevented the decrease in AsA/dehydroascorbic acid and delayed the accumulation of total soluble solids/titratable acidity, but also altered phytohormone levels differently.

A fresh-cut papaya freshness prediction model based on partial least squares regression and support vector machine regression.

This study investigated the physicochemical and flavor quality changes in fresh-cut papaya that was stored at 4 °C. Multivariate statistical analysis was used to evaluate the freshness of fresh-cut papaya. Aerobic plate counts were selected as a predictor of freshness of fresh-cut papaya, and a prediction model for freshness was established using partial least squares regression (PLSR), and support vector machine regression (SVMR) algorithms.

Robust Thiazole-Linked Covalent Organic Frameworks for Water Sensing with High Selectivity and Sensitivity.

The rational design of covalent organic frameworks (COFs) with hydrochromic properties is of significant value because of the facile and rapid detection of water in diverse fields. In this report, we present a thiazole-linked COF (TZ-COF-6) sensor with a large surface area, ultrahigh stability, and excellent crystallinity. The sensor was synthesized through a simple three-component reaction involving amine, aldehyde, and sulfur.

Mechanistic insights into nitrogen-induced changes in pasting characteristics of rice during storage based on proteomics analysis.

Nitrogen application delays rice quality deterioration due to changes in its pasting characteristics; however, the underlying mechanisms remain unclear. Using a label-free quantitative proteomics approach, we identified differentially expressed proteins (DEPs) during storage in paddy rice treated with different nitrogen levels. On combining the changes in physiological indicators, high-nitrogen treatment was found to downregulate β-1,3-glucanase, reduce the decomposition of cell wall components, downregulate three proteins involved in starch metabolism, decrease the range of the amylose content and increase the range of the amylopectin, upregulate three proteins related to the lysosomal pathway, and enhance glutelin degradation.

ProS-GNN: Predicting effects of mutations on protein stability using graph neural networks.

Predicting protein stability change upon variation through a computational approach is a valuable tool to unveil the mechanisms of mutation-induced drug failure and develop immunotherapy strategies. Some previous machine learning-based techniques exhibit anti-symmetric bias toward destabilizing situations, whereas others struggle with generalization to unseen examples. To address these issues, we propose a gated graph neural network-based approach to predict changes in protein stability upon mutation.

Associations among multidomain lifestyles, chronic diseases, and dementia in older adults: a cross-sectional analysis of a cohort study.

Background: Unhealthy lifestyles and chronic diseases are commonly seen and treatable factors in older adults and are both associated with dementia. However, the synergistic effect of the interaction of lifestyles and chronic diseases on dementia is unknown.

Methods: We determined independent associations of multidomain lifestyles and chronic diseases (cerebrovascular disease, diabetes, and hypertension) with dementia and examined their synergistic impact on dementia among older adults.

Prediction of vaginal birth after cesarean in China.

Objective: This study aimed to develop and validate a prediction model of vaginal birth after cesarean delivery (VBAC) in China.

Methods: A nomogram for effective prediction of VBAC of singleton, cephalic and one previous low-transverse cesarean section deliveries was created by comparing the combinations of ultrasonographic and non-ultrasonographic factors from five hospitals between 2018 and 2019.

Results: A total of 1066 women were included.

Walking or jogging during pregnancy increases the success rate of vaginal birth after cesarean delivery: A multicenter, retrospective cohort study.

Objective: Exercise during pregnancy has been proven to reduce the risk of cesarean delivery. However, few studies have examined the relationship between walking or jogging during pregnancy and the success rate of vaginal birth after cesarean delivery (VBAC). This multicenter, retrospective cohort study aimed to determine the association between walking or jogging during pregnancy and the success rate of VBAC in women who underwent a trial of labor after cesarean delivery.

Effect of different doses of nitrogen fertilization on bioactive compounds and antioxidant activity of brown rice.

Brown rice as a whole grain food is associated with various chronic diseases' reduced risks. In this study, the effects of different doses of nitrogen fertilization (0, 160, 210, 260, 315, and 420 kg N/ 100 m) on bioactive compounds and antioxidant activity of brown rice (yanfeng47) were investigated. At nitrogen level of 210-260 kg N/100 m, the content of TFC (302.

Dynamic regulation and key roles of ribonucleic acid methylation.

Ribonucleic acid (RNA) methylation is the most abundant modification in biological systems, accounting for 60% of all RNA modifications, and affects multiple aspects of RNA (including mRNAs, tRNAs, rRNAs, microRNAs, and long non-coding RNAs). Dysregulation of RNA methylation causes many developmental diseases through various mechanisms mediated by -methyladenosine (mA), 5-methylcytosine (mC), -methyladenosine (mA), 5-hydroxymethylcytosine (hmC), and pseudouridine (Ψ). The emerging tools of RNA methylation can be used as diagnostic, preventive, and therapeutic markers.

Polyphenol Composition and Antioxidant Activity of Rice Cultivars and Intake Status.

rice is produced mainly in northeast China, Japan, and the Korean Peninsula. Polyphenols and flavonoids are the main antioxidants in rice. This study reported the polyphenol content and antioxidant activity of nine brown and white rice cultivars.

Metabolic Variations in Brown Rice Fertilised with Different Levels of Nitrogen.

Nitrogen is a necessary element for plant growth; therefore, it is important to study the influence of N fertilisers on crop metabolites. In this study, we investigate the variability of endogenous metabolites in brown rice fertilised with different amounts of nitrogen. We identified 489 metabolites in brown rice.

Ozone treatment promotes physicochemical properties and antioxidant capacity of fresh-cut red pitaya based on phenolic metabolism.

Pitaya is an important fresh-cut product in the global fruit market. The health benefits of fresh-cut red pitaya fruit are attributed to its unique phenolic content and other antioxidants, but the fruit is highly susceptible to spoilage which causes a decline in nutritional quality. In this study, we monitored changes in quality and phenolic compounds of pitaya fruit treated with gaseous ozone during storage at 8 ± 2C for 4 days.

The intervention effect of mental health knowledge integrated into ideological and political teaching on college students' employment and entrepreneurship mentality.

In order to analyze the intervention effect of integrating mental health knowledge into ideological and political teaching on college students' employment and entrepreneurship mentality, this paper proposes a study to predict the effect of integrated intervention. This research mainly investigates the ideological and psychological conditions of college students from divorced families through questionnaires, compares and analyzes the classification and statistical results of the survey data between groups and within groups, and analyzes the reasons for the ideological and psychological problems of college students. The experimental results show that 30% of college students from divorced families and college students from non-divorced families responded that they do not feel comfortable in places with many people, and the difference between the groups is not significant.

Mechanism of the Micellar Solubilization of Curcumin by Mixed Surfactants of SDS and Brij35 via NMR Spectroscopy.

The micellar solubilization mechanism of curcumin by mixed surfactants of SDS and Brij35 was investigated at the molecular scale by NMR spectroscopy. Through the investigation of the micelle formation process, types and structures of mixed micelles and solubilization sites, the intrinsic factors influencing the solubilization capacity were revealed. For systems with α = 0.

Degradation of malathion and carbosulfan by ozone water and analysis of their by-products.

Background: Treatment by ozone water is an emerging technology for the degradation of pesticide residues in vegetables. The ozone dissolved in water generates hydroxyl radicals ( OH), which are highly effective in decomposing organic substances, such as malathion and carbosulfan.

Results: We found that washing pak choi with 2.

Trimester-specific prenatal heavy metal exposures and sex-specific postpartum size and growth.

Background: There has been limited research considering the effects of prenatal exposure to multiple heavy metals on early childhood size and growth.

Objective: We evaluated prenatal exposures to 15 heavy metals in association with measures of weight, length, and head circumference (HC) measured at birth, and 1, 3 and 6 months of age in a study of 358 mother-child pairs.

Methods: Urinary concentrations were measured in the first and third trimesters of pregnancy and examined, using sex-stratified general linear models, in association with average standardized size and changes in size (growth) over the first 6 months of life.