Ribosome engineering of Myxococcus xanthus for enhancing the heterologous production of epothilones.

Background: Ribosome engineering is a semi-empirical technique used to select antibiotic-resistant mutants that exhibit altered secondary metabolism. This method has been demonstrated to effectively select mutants with enhanced synthesis of natural products in many bacterial species, including actinomycetes. Myxobacteria are recognized as fascinating producers of natural active products.

Depth-prior-based range-gated 3D imaging method: integration of optical property into visual learning.

3D Range-gated Imaging (3DRGI) has great potential for long-range detection in adverse weather conditions. Recently, vision-guided 3DRGI has brought new perspectives to this area as it overcomes hardware limitations and greatly increases flexibility. However, existing vision-guided methods do not consider the optical properties of range-gated imaging, which results in low accuracy.

Depth-prior-based LiDAR point cloud de-noising method leveraging range-gated imaging.

Light Detection and Ranging (LiDAR) has been widely adopted in modern self-driving vehicles and mobile robotics, providing 3D information of the scene and surrounding objects. However, LiDAR systems suffer from many kinds of noise, and its noisy point clouds degrade downstream tasks. Existing LiDAR point cloud de-noising methods are time-consuming or cannot deal with the noise caused by occlusions or penetrating transparent surfaces.

Research on CO-WAG in Thick Reservoirs: Geological Influencing Factors and Random Forest Importance Evaluation.

In the development process of thick reservoirs, the impact of various geological factors on the effectiveness of the CO water alternating gas (CO-WAG) flooding technology remains unclear. This paper establishes multiple CO-WAG flooding models for thick reservoirs to study the effects of sedimentary rhythm, dip angle, matrix permeability, high-permeability streaks (HPS), and barrier layers on the effectiveness of CO-WAG flooding and then uses the random forest algorithm to rank the importance of these geological factors. The results show that different geological factors have varying degrees of impact on the distribution of water and gas migration and recovery rates during the CO-WAG flooding process.

Enhanced production of aspochalasin D through genetic engineering of Aspergillus flavipes.

Aspochalasin D (AD) belongs to the polyketide-amino acid hybrid natural products with anti-cancer, anti-bacterial, and anti-fouling bioactivities. However, the low production limits its further application. In this study, AD was separated and identified from Aspergillus flavipes 3.

SwinOCSR: end-to-end optical chemical structure recognition using a Swin Transformer.

Optical chemical structure recognition from scientific publications is essential for rediscovering a chemical structure. It is an extremely challenging problem, and current rule-based and deep-learning methods cannot achieve satisfactory recognition rates. Herein, we propose SwinOCSR, an end-to-end model based on a Swin Transformer.

Development of the general chapters of the Chinese Pharmacopoeia 2020 edition: A review.

The Chinese Pharmacopoeia 2020 edition was reviewed and approved by the National Medical Products Administration and the National Health Commission of the People's Republic of China in July 2020. The current edition was officially implemented on December 30, 2020. The general chapters of the Chinese Pharmacopoeia discuss the general testing methods and guidelines, which are the common requirements and basis for the implementation of drug standards in the Chinese Pharmacopoeia.

Aquatic ecotoxicity of an antidepressant, sertraline hydrochloride, on microbial communities.

Sertraline hydrochloride (Ser-HCl), a widely used antidepressant, becomes an aquatic contaminant via metabolic excretion and improper disposal; however, it is unknown how Ser-HCl affects aquatic microbial communities. The present study investigated the effects of Ser on the structures of aquatic microbial communities via high-throughput sequencing analyses. Ser-HCl treatment inhibited the growth of two model algae (the green alga, Chlorella vulgaris, and the cyanobacterium, Microcystis aeruginosa) and decreased the chlorophyll a (Chl-a) concentration in the microcosm to reduce the photosynthetic efficiency.

[Single-use Medical Devices Re-processing: Risk Assessment and Quality Control Technologies].

The reuse of high-cost single-use medical devices (SUD) is permitted in many countries, such as the United States, Germany and the United Kingdom, but strict regulatory requirements must be met. In addition to regulatory policies and regulations, such as market access mode and special requirements on Good Manufacture Practice (GMP), there are strict technical requirements on the potential risk control and quality assurance system. Therefore, effective risk assessment and risk control technology are the keys to ensure effective quality control and safe use of SUDs.

[Cell Count of Mouse Blastocyst on Pre-clinical Evaluation of Safety of Medical Devices in Assisted Reproductive Technologies].

Various types of medical devices used in assisted reproductive technologies (ART) should be detected for their safety by strict biological assays. Mouse embryo assay(MEA)has been recognized as one of the most important and standardized methods with the threshold more than 80% of blastocyst formation rate (BR) after 96 h culture of fertilized eggs. The disadvantage using BR for embryonic quality control has been concerned as it is ubiquitously dependent of embryonic morphology and the detailed data including molecular and genetic information is obviously missing and incomplete.

Responses of unicellular alga Chlorella pyrenoidosa to allelochemical linoleic acid.

Linoleic acid (LA), is the product of secondary metabolism secreted from Microcystis aeruginosa, and it exhibits allelopathic activity against eukaryotic algae. However, information about on the mechanisms associated with the inhibition of algal activity by LA is limited. In this study, Chlorella pyrenoidosa was treated with LA (20-120 μg L) for 4 days, and its growth inhibition and physiological responses were examined for potential toxic mechanisms.

[Single-use Medical Devices Re-processing: Regulatory Status Quo].

Some single-use medical devices are reprocessed and reused in some countries in the world, but the regulatory approach is different, and in some countries it isn't regulated yet. In this article, the regulatory status quo of single-use medical devices is reviewed. The regulatory development, important regulatory documents and regulatory approaches of single-use medical device reprocessing in the United States, Germany and the UK are introduced.

A reference human genome dataset of the BGISEQ-500 sequencer.

BGISEQ-500 is a new desktop sequencer developed by BGI. Using DNA nanoball and combinational probe anchor synthesis developed from Complete Genomics™ sequencing technologies, it generates short reads at a large scale. Here, we present the first human whole-genome sequencing dataset of BGISEQ-500.

Tissue toxicity following the vaginal administration of nanosilver particles in rabbits.

Nanosilver particles are used in various clinical settings because of their antibacterial properties. However, their safety evaluation when used for gynaecological disorders has not been established. Nanosilver particles were administrated in the vagina of New Zealand rabbits, and the pathological appearance of the surrounding tissue was examined by hematoxylin-eosin staining and transmission electron microscopy (TEM) after 1 and 3 days of treatment.

Premarket Regulation of Tissue Engineered Medical Products in China.

Tissue engineered medical products (TEMPs) use state-of-the-art technologies and offer the patients with alternative clinical options for diseases that conventional treatments may fail or be incompetent. However promising, this technology is comparatively new with very limited hands-on experiences with both manufacturing and clinical therapy. Of great significance to products with such complexity and novelty is the establishment of a complete jurisdiction framework and a standardization database so that the safety of the technique in clinical treatment can be ensured.

[Analysis of the National Quality Inspection for Medical Devices].

This paper analyses overall situation of the national quality inspection for medical devices in recent 13 years. The statistics cover the inspected varieties, sampling quantity and quality status. The achievements and suggestions are provided, which are helpful for future work.

One-Step Reverse-Transcription FRET-PCR for Differential Detection of Five Ebolavirus Species.

Ebola is an emerging infectious disease caused by a deadly virus belonging to the family Filoviridae, genus Ebolavirus. Based on their geographical distribution, Ebolavirus has been classified into total five species so far, mainly Zaire, Sudan, Taï Forest, Bundibugyo and Reston. It is important to be able to differentiate the Ebolavirus species as they significantly differ in pathogenicity and more than one species can be present in an area.

[Situation and suggestions on IVD industrial standards].

This paper briefly introduces the working procedure of in vitro diagnostic products (IVD) industrial standards, and elaborates the importance of professional standards for production and supervision. Based on the analysis of working progress during the past 10 years, some problems and countermeasures on project setting, participation, standard material, personnel training, work cycle are put forward, which are helpful for the future development of the IVD.

[The preparation and bioactivity assessment of gene recombinant fibrin-binding brain derived neurotrophic factor].

Using the E. coli, we fabricated the gene reconstructed brain derived neurotrophic factor with a fibrin binding domain (FBD-BDNF). We then tested the neurotrophic bioactivity and fibrin-binding ability of the FBD-BDNF.

[Comments and suggestions on the medical device standardization system from the view point of medical devices test].

Medical device test is a very important step of medical device registration processes and is also a procedure to confirm whether the medical devices comply with applicable standards. The standardization system is imperfect yet in China due to the complexity of medical devices and the specialty of medical device industry. In this circumstance, the medical device standard is not only the important criteria for the medical devices registration, but also an important practical procedure of the medical device registration.