Chronological Management of Adjuvant Effect for Optimized mRNA Vaccine Inspired by Natural Virus Infection.

The efficacy and safety of mRNA vaccines both rely on a fine-tuning of specific humoral and cellular immune responses. Instead of adjustments in vaccine component, we proposed a concept of chronological management of adjuvant effect to modulate the adaptive immune potency and preference inspired by natural virus infection. By simulating type I interferon expression dynamics during viral infection, three vaccine strategies employing distinct exposure sequences of adjuvant and mRNA have been developed, namely , , and .

Rational administration sequencing of immunochemotherapy elicits powerful anti-tumor effect.

As a research hotspot, immune checkpoint inhibitors (ICIs) is often combined with other therapeutics in order to exert better clinical efficacy. To date, extensive laboratory and clinical investigations into the combination of ICIs and chemotherapy have been carried out, demonstrating augmented effectiveness and broad application prospects in anti-tumor therapy. However, the administration of these two treatment modalities is usually randomized or fixed to a given chronological order.

An analysis of the biopharmaceutical behaviour of proton pump inhibitors with different physicochemical properties.

At present, little information on the biopharmaceutical behaviour of proton pump inhibitors (PPIs) describing their absorption and biodistribution in vivo has been reported because the extreme instability of PPIs in the gastrointestinal environment makes it difficult to analyze such behaviour. In this work, a modified rat in situ intestinal perfusion model was employed to investigate absorption in the gastrointestinal tract and subsequent biodistribution of several PPIs (ilaprazole, esomeprazole and rabeprazole), which have different physicochemical properties. Our data indicated that PPIs exhibited significantly enhanced absorption rates in the whole intestine, including the duodenum, jejunum, ileum and colon, corresponding to the increase in the oil-water partition coefficient (LogP).

The study of intestinal absorption and biodistribution in vivo of proton pump inhibitors.

The major therapeutic strategy for acid-related gastrointestinal diseases in clinic is to reduce the excretion of gastric acid by oral administration of proton-pump inhibitors (PPIs). However, it is quite a challenge to study the oral absorption behaviors of PPIs considering their extreme instability under gastrointestinal environment. As a result, little information has been reported on PPI oral absorption so far, hindering the further development of PPI-contained oral preparations.

Selective Intratumoral Drug Release and Simultaneous Inhibition of Oxidative Stress by a Highly Reductive Nanosystem and Its Application as an Anti-tumor Agent.

Excessive oxidative stress is always associated with the serious side effects of chemotherapy. In the current study, we developed a vitamin E based strongly reductive nanosystem to increase the loading efficiency of docetaxel (DTX, DTX-VNS), reduce its side toxicity and enhance the antitumor effect. We used Förster Resonance Energy Transfer (FRET) to reveal the and fate of DTX-VNS over time.

Preparation and evaluation of regioselectively substituted amylose derivatives for chiral separations.

Six novel regioselectively substituted amylose derivatives with a benzoate at 2-position and two different phenylcarbamates at 3- and 6-positions were synthesized and their structures were characterized by H nuclear magnetic resonance (NMR) spectroscopy. Their enantioseparation abilities were then examined as chiral stationary phases (CSPs) for high-performance liquid chromatography (HPLC) after they were coated on 3-aminopropyl silica gels. Investigations indicated that the substituents at the 3- and 6-positions played an important role in chiral recognition of these amylose 2-benzoate serial derivatives.

[Study on the chromatographic fingerprints of Reineckia carnea from different habitats by HPLC-ELSD].

Objective: To investigate the HPLC-ELSD fingerprints of Reineckia carnea from different habitats and provide a theoretical evidence to evaluate its quality.

Methods: The chromatographic conditions were Diamonsil (R) C18 column (4.6 mm x 250 mm, 5 microm) by linear gradient elution using water and acetonitrile as mobile phase at a flow rate of 0.

[Preparation and evaluation of amylose and cellulose tris (3-trifluoromethylphenylcarbamates)-based chiral stationary phases].

To broaden the category of polysaccharide-based chiral stationary phases (CSPs), coated CSPs based on tris (3-trifluoromethylphenylcarbamates) of amylose and cellulose were prepared for enantioseparation in high performance liquid chromatography. Their performances were evaluated by eight racemates using hexane-isopropanol as mobile phase. Compared with the most widely used, commercially available polysaccharide-based CSPs, Chiralpak AD and Chiralcel OD, utilizing tris (3,5-dimethylphenylcarbamates) of amylose and cellulose as the chiral selector, respectively, the obtained CSPs exhibited lower enantioseparation abilities.

Improved preparation of chiral stationary phases via immobilization of polysaccharide derivative-based selectors using diisocyanates.

The classical method for the preparation of immobilized polysaccharide-based chiral stationary phases (CSPs) with a diisocyanate was improved. Cellulose or amylose was directly coated onto 3-aminopropyl silica gel after it was dissolved in a mixture of N,N-dimethylacetamide, LiCl, and pyridine, then immobilized onto silica gel with a diisocyanate, and finally allowed to react with an excess of corresponding isocyanate. Four polysaccharide derivatives, 3,5-dimethylphenylcarbamate and 3,5-dichlorophenylcarbamate of cellulose, and 3,5-dimethylphenylcarbamate and 5-chloro-2-methylphenylcarbamate of amylose, were immobilized onto silica gel utilizing this method.