Publications by authors named "Zhaofei Fan"

Nonnative invasive plant species (NNIPS) cause significant damage to the native forest ecosystems in the southern United States forestlands, such as habitat degradation, ecological instability, and biodiversity loss. Taking the state of Alabama as an example, we used more than 5,000 permanent United States Department of Agriculture-Forest Service's Forest Inventory and Analysis (FIA) plots measured between 2001 and 2019 over three measurement cycles to test the suitable modeling unit for quantifying invasion patterns and associated factors for regional NNIPS monitoring and management. NNIPS heavily infest Alabama's forestlands, and forestlands plagued with at least one NNIPS have increased over time: 41.

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Understory vegetation accounts for a large proportion of floral diversity. It provides various ecosystem functions and services, such as productivity, nutrient cycling, organic matter decomposition and ecosystem self-regeneration. This review summarizes the available literature on the current status and progress of the ten most studied branches of understory vegetation on both its structural and functional aspects based on global climate change and forest management practices.

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Global climate change and the increase in the frequency and intensity of drought have led to widespread forest decline and tree mortality. Studying the resilience components of tree growth to drought, including resistance (R), recovery (R), and resilience (R) and the influencing factors, helps assess forests' production and ecological stability under a changing climate. This study analyzed the responses of three resilience components of natural Mongolian pine (Pinus sylvestris var.

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The abundance of cavity trees varies greatly due to the stochastic nature of cavity formation processes and involved disturbance agents. At small spatial scales such as a stand or plot, cavity tree abundance is extraordinarily difficult to predict precisely using tree and stand factors. In this study we used resampling methods to simulate the effect of spatial scale on cavity tree density (CTD) estimation using cavity tree data collected from a long-term forest experimental project.

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The invasion of Chinese tallow ( (L.) Small) is a serious threat to the endangered slash pine () flatwood ecosystem in the Gulf of Mexico Coastal Plain, United States. Prescribed fire in combination with vegetation management has been suggested as a preferred approach for mitigating Chinese tallow invasion and restoring this endangered ecosystem.

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Olive flounder (Paralichthys olivaceus) is a commercially important flatfish species cultured in East Asia. Female flounders generally grow more rapidly than males, therefore control of the sex ratio seems to be a proposed way to increase production. However, the sex determination gene and sex determination mechanism have yet been elucidated.

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As an important maricultured fish, the olive flounder Paralichthys olivaceus shows sex-dimorphic growth. Thus, the molecular mechanisms involved in sex control in P. olivaceus have attracted researchers' attention.

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Androgens are critical hormones that regulate sex differentiation, sexual maturation, and spermatogenesis in vertebrates, which is mainly mediated by androgen receptors (ARs). Reports on transcript variants of ar (AR encoding gene) in human are almost always associated with cancers and androgen insensitivity syndrome. However, the knowledge of ar variants in teleosts is scarce.

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The P450 side-chain cleavage enzyme, P450scc (Cyp11a) catalyzes the first enzymatic step for the synthesis of all steroid hormones in fish. To study its roles in gonads of the olive flounder , an important maricultured fish species, we isolated the genomic DNA sequence of 1396 bp, which consists of 5 exons and 4 introns. Semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) results indicated that the flounder was exclusively expressed in gonad and head kidney tissues.

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Steroidogenic acute regulatory protein 2 (StAR2) is a key protein in transporting cholesterol from the outer mitochondria membrane to the inner mitochondria membrane for sex steroid synthesis. In this study, two StAR2 gene isoforms, StAR2a and StAR2b, were isolated from the olive flounder Paralichthys olivaceus gonads. Semi-quantitative RT-PCR results indicated that their expression levels were higher in testis than those in ovary.

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The sex ratio of olive flounder Paralichthys olivaceus is sensitive to temperature or exogenous hormone exposures in the period of gonadal differentiation. Among sex-related genes, cyp19a, encoding cytochrome P450 aromatase, exhibits significant sex-dimorphic expression pattern and plays an important role in fish gonadal differentiation and development. The present study investigated the expression levels and promoter methylation dynamics of cyp19a and its regulators (nr5a2 and nr0b1), and sex-steroid hormone levels during flounder gonadal differentiation under the treatments of high temperature and estradiol-17β (E2).

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Plant fibrous material is a good resource in textile and other industries. Normally, several kinds of plant fibrous materials used in one process are needed to be identified and characterized in advance. It is easy to identify them when they are in raw condition.

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Olive flounder Paralichthys olivaceus is an important commercially cultured marine flatfish in China, Korea, and Japan. Gynogenesis, via meiogynogenesis and mitogynogenesis, shows advantages in breeding and sex control, but the low survival rate, especially for mitogynogenesis, limits its application. In this study, we sequenced the embryo transcriptomes of gynogenetic haploid, meiogynogenetic diploid, mitogynogenetic diploid, and common diploid flounder and investigated their respective genetic characteristics by analyzing differentiated expressed genes.

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The rock bream (Oplegnathus fasciatus) is considerably one of the most economically important marine fish in East Asia and has a unique neo-Y chromosome system that is a good model to study the sex determination and differentiation in fish. In the present study, we used Illumina sequencing technology (HiSeq2000) to sequence, assemble and annotate the transcriptome of the testis and ovary tissues of rock bream. A total of 40,004,378 (NCBI SRA database SRX1406649) and 53,108,992 (NCBI SRA database SRX1406648) high quality reads were obtained from testis and ovary RNA sequencing, respectively, and 60,421 contigs (with average length of 1301 bp) were obtained after de novo assembling with Trinity software.

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WNT4 (wingless-type MMTV integration site family, member 4) is regarded as a key regulator of gonad differentiation in mammalians. However, the potential role of wnt4 in teleosts during gonad differentiation and development is still unclear. The full-length cDNA sequence of wnt4 in olive flounder (Paralichthys olivaceus) was obtained using RACE (rapid amplification of cDNA ends) technique.

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Olive flounder (Paralichthys olivaceus) is an important commercially cultured marine flatfish in China, Korea, and Japan, of which female grows faster than male. In order to explore the molecular mechanism of flounder sex determination and development, we used RNA-seq technology to investigate transcriptomes of flounder gonads. This produced 22,253,217 and 19,777,841 qualified reads from ovary and testes, which were jointly assembled into 97,233 contigs.

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