Molecular detection and characterization of Coxiella burnetii in aborted samples of livestock in China.

Coxiella burnetii is the causative agent of zoonotic Q fever. Animals are the natural reservoirs of C. burnetii, and domestic livestock represent the major sources of human infection.

Comparison of the immune effects of the MOMP antigen displayed in different parts of bacterial ghosts.

Bacterial ghosts (BGs) are promising vaccine platforms owing to their high adjuvant properties and delivery efficiency. Heterologous antigens can be anchored to different parts of BGs using genetic engineering strategies to prepare vaccines. However, several key issues need to be resolved, including the efficient preparation of BGs and determining the optimal anchoring position of exogenous antigens in the BGs.

Seroprevalence of Chlamydia abortus and Brucella spp. and risk factors for Chlamydia abortus in pigs from China.

Chlamydiosis and brucellosis induced abortions have resulted in significant economic losses in the global livestock industry. Although there have been numerous reports on these two diseases in ruminants in China, limited information is available regarding the prevalence of Chlamydia abortus (C. abortus) and Brucella spp.

Gut microbiota changes in horses with Chlamydia.

Background: Zoonotic diseases pose a significant threat to public health. Chlamydia, as an intracellular pathogen, can colonize the intestinal tract of humans and animals, changing the gut microbiota. However, only a few studies have evaluated alterations in the gut microbiota of horses infected with Chlamydia.

The Post-Antibiotic Era: A New Dawn for Bacteriophages.

Phages are the most biologically diverse entities in the biosphere, infecting specific bacteria. Lytic phages quickly kill bacteria, while lysogenic phages integrate their genomes into bacteria and reproduce within the bacteria, participating in the evolution of natural populations. Thus, lytic phages are used to treat bacterial infections.

Jatrolignans C and D: New Neolignan Epimers from .

Two new neolignans jatrolignans, C () and D (), a pair of epimers, were isolated from the whole plants of L. (Euphorbiaceae). Their structures were determined with HRESIMS, IR, and NMR data analysis, and electronic circular dichroism (ECD) experiments via a comparison of the experimental and the calculated ECD spectra.

Chlamydia abortus OmcB protein is essential for adhesion to host cells.

Chlamydia abortus (C. abortus) is one of the most important zoonotic pathogens, causing a number of serious diseases. The adhesion of C.

Seroprevalence of Chlamydia abortus infection in yak (Bos grunniens) in Tibet, China.

Chlamydia spp. are prevalent zoonotic pathogens that infect a wide variety of host species. Chlamydia abortus (C.

Specific Detection and Differentiation Between and by a Duplex Recombinase Polymerase Amplification Assay.

Brucellosis is a highly contagious zoonosis caused by a species under the genus . A duplex recombinase polymerase amplification (Duplex RPA) assay for the specific detection of and was developed in this study. Primers were designed targeting hypothetical protein genes and membrane transporter genes of and , respectively.

Lipofectamine enhances Chlamydia infectivity in cell culture.

Cultivation of Chlamydia species in cell lines requires centrifugation of the inoculum onto diethylaminoethyl-dextran-pretreated cell monolayers to improve the infection efficiency. Here we report that the addition of DNA transfection reagent Lipofectamine in the inoculum significantly enhances the infectivity of Chlamydia abortus in mouse fibroblast McCoy cells, with an infection efficiency equivalent to that of the centrifugation method. Similar enhancement effects of Lipofectamine on the infectivity of C.

Detection of and in the Endangered Crested Ibis ().

Chlamydia spp. are a group of obligate intracellular pathogens causing a number of diseases in animals and humans. Avian chlamydiosis (AC), caused by Chlamydia psittaci (C.

Establishment of a recombinase polymerase amplification (RPA) assay for the detection of Brucella spp. Infection.

Brucellosis is a worldwide re-emerging zoonosis. It has an economic impact due to abortion and loss of fertility in livestock. In this study, Real-time recombinase polymerase amplification (RT-RPA-BP26) targeting Brucella spp.

First Report of in Farmed Fur Animals.

, a globally distributed obligate intracellular bacterium, has attracted increasing interest according to its veterinary importance and zoonotic nature. can infect a variety of animals and cause foetal loss in livestock resulting in economic loss. In this study, the samples collected from two farms of foxes (n=20), raccoon dogs (n=15) and minks (n=20), were investigated by - and species-specific real-time PCR.

Synonymous Codon Usages as an Evolutionary Dynamic for .

The family of contains a group of obligate intracellular bacteria that can infect a wide range of hosts. The evolutionary trend of members in this family is a hot topic, which benefits our understanding of the cross-infection of these pathogens. In this study, 14 whole genomes of 12 species were used to investigate the nucleotide, codon, and amino acid usage bias by synonymous codon usage value and information entropy method.

The Burden of Human Papillomavirus and Chlamydia trachomatis Coinfection in Women: A Large Cohort Study in Inner Mongolia, China.

Background: Chlamydia trachomatis may coinfect with human papillomavirus (HPV) and complicate the cervical pathogenesis. This study aimed to evaluate the prevalence, risk factors, and clinical outcomes of HPV/C. trachomatis coinfection in women from Inner Mongolia, China.

Prevalence, Diagnosis, and Vaccination Situation of Animal Chlamydiosis in China.

Since the first case of infection in duck had been reported in 1956 and the first case from domestic animal had been reported in 1979 in China, the chlamydia prevalence in China was heavily according to the published data. The a in avian prevalence has been reported at least 11 provinces, in sheep and goats at least 11 provinces, in swine at least 15 provinces, in cows at least 13 provinces and in yaks at least 5 provinces with result of IHA detection. Different diagnostic method such as CFT, ELISA and ABC-ELISA (avidin-biotin-complex ELISA) had been established besides IHA.

Genetic Characterization of Animal Isolates from Northwest Region in China.

Animal brucellosis is a reemerging disease in China, particular in northwest China. The species (even genus) are highly conserved; therefore the use of Multilocus sequencing typing (MLST: based on conserved housekeeping loci) is more suitable for discrimination at species or biovar level on . In this study, MLST was used to analyze the characterization of from sheep and yaks during 2015 and 2016.

Enzootic situation and molecular epidemiology of Brucella in livestock from 2011 to 2015 in Qingyang, China.

A large-scale survey was conducted in domestic animal populations from 2011 to 2015 in Qingyang, China. A total of 448,398 animals from different districts of Qingyang were tested for the presence of Brucella-specific antibodies using the Rose Bengal Plate Test (RBPT) and the Standard Agglutination Test (SAT). From 2011 to 2015, the yearly average positive rates were between 0.

Whole-Genome Sequences of Strain QH61, Isolated from Yak in Qinghai, China.

The facultative intracellular Gram-negative bacterium causes brucellosis in domestic and wild mammals. QH61 was isolated from a yak suffering from abortion in 2015 in Qinghai, China. Here, we report the whole-genome sequence of strain QH61.

Whole-Genome Sequences of Strain QY1, Isolated from Sheep in Gansu, China.

The facultative intracellular Gram-negative bacterium causes brucellosis in domestic and wild mammals, and it is a dominant pathogen responsible for human disease. This study reports the whole-genome sequencing of strain QY1, isolated from sheep suffering from abortion and arthritis in 2015 in Gansu, China.

Whole-Genome Sequence of Strain GN6 Isolated from Aborted Yak Fetus.

The obligate intracellular Gram-negative bacterium is one of the causative agents of abortion and fetal loss in sheep, goats, and cattle in many countries. It also affects the reproductivity of yaks (). This study reports the whole-genome sequence of strain GN6, which was isolated from aborted yak fetus in Qinghai-Tibetan Plateau, China.

Identification and characterization of Chlamydia abortus isolates from yaks in Qinghai, China.

Recently, the yak population has exhibited reproductive disorders, which are considered to be associated with Chlamydia abortus (C. abortus) in Qinghai, China. In this study, a total of 9 aborted fetuses (each from a different herd) and 126 vaginal swab samples from the 9 herds were collected and analyzed.

Molecular investigation of bovine viral diarrhea virus infection in yaks (Bos gruniens) from Qinghai, China.

Background: Bovine viral diarrhea virus (BVDV) is a pestivirus which infects both domestic animals and wildlife species worldwide. In China, cattle are often infected with BVDV of different genotypes, but there is very limited knowledge regarding BVDV infection in Chinese yaks and the genetic diversity of the virus. The objectives of this study were to detect viral infection in yaks in Qinghai, China and to determine the genotypes of BVDV based on analysis of the 5'untranslated region (5'UTR) and N-terminal protease (N(pro)) region.