[The effect of seawater on expression of protease-activated receptor 2 in adenocarcinoma of lung cell line A549 cells].

Objective: To investigate the effect of seawater on expression of protease-activated receptor 2 (PAR-2) in adenocarcinoma of lung cell line A549 cells, and the inflammatory injury on A549 cells induced by seawater.

Methods: A549 cells were randomly divided into four groups: control group, 2 hours group, 4 hours group, 8 hours group, in which cells were treated with seawater for 2, 4 and 8 hours respectively. After seawater treatment, cells were collected for real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting analysis to determine the expression of PAR-2 mRNA and its protein.

[Near-infrared luminescence of Yb3+-chrome blue-black R complex].

In the present work, sensitized near-infrared fluorescence from Yb3+-chrome blue-black R (CBR) complex was studied. It was found that Yb3+ forms 1:2 complex with CBR in natural ethanol solution. The visible fluorescence from CBR was quenched when the complex forms, while the near-infrared fluorescence from Yb3+ was greatly enhanced.

Determination of chlorine in atmosphere by kinetic spectrophotometry.

A kinetic method for determination of chlorine in air was described in the present work. The method based on fading of methyl orange (MO) containing solution in air absorption process. A determination limit of 2.