Study on diagnosis criteria of fire-heat syndrome based on receiver operating characteristic curve and principal component analysis.

Objective: To establish the diagnostic quantitative criteria for fire-heat syndrome (FHS) of Chinese medicine (CM) based on the receiver operating characteristic (ROC) curve and principal component analysis (PCA).

Methods: The symptoms and signs of FHS cases and healthy subjects from Guangzhou, Henan and Hunan of China were collected through questionnaire, and the diagnostic quantitative score tables were established for the three regions, respectively, with the method of maximum likelihood analysis. The homogeneity test was then performed on the diagnostic score tables for the three regions with ROC curve, and the diagnostic efficiency of diagnostic score tables for the three regions was compared with the prospective test and retrospective test.

[Study on effects of curcumin on expressions of PDGF-BB, PDGFRbeta and ERK1 of HSC].

Objective: To observe the effects of curcumin on expression of PDGF-BB, PDGFRbeta and ERK1 of rat Hepatic stellate cell (HSC-T6).

Methods: The cultured HSC were divided into control group, each curcumin treating group. The influence of curcumin on HSC proliferation was detected by MTT assay.

[Radioprotective effect of aloe polysaccharides on three non-tumor cell lines].

Background & Objective: Our previous study showed that aloe polysaccharides (AP) could evidently decrease the mortality of irradiated mice mainly through increasing the amount of hemocytes and ameliorating immune function of mice. Whether AP can protect the cells in vitro from irradiation damage is unknown. This study was to explore radioprotective effect of AP on 3 non-tumor cell lines, and its effect on cell cycle.

Aloe polysaccharides mediated radioprotective effect through the inhibition of apoptosis.

Polysaccharides from aloe are always considered an effective radioprotector on irradiation-induced skin damage. The aim of this study was to determine if aloe polysaccharides (AP) have radioprotective effects on normal human cells in vitro and mouse survival in vivo and to explore the mechanism. Pretreatment with 50 microg/ml AP could improve the surviving fraction at 2 Gy (SF2) of three normal cell lines 293, ECV304, and C.

Protective effects of polydatin against CCl(4)-induced injury to primarily cultured rat hepatocytes.

AIM:To investigate the protective effects of polydatin (PD) against injury to primarily cultured rat hepatocytes induced by CCl(4).METHODS:Rat hepatocytes were separated by methods of liver infusion in vivo and cultured medium (7.5X10(5) cells/mL).