Publications by authors named "Zhao-ling Liang"
[Quantitative detection of hepatitis B virus covalently closed circular DNA in sera of chronic hepatitis B patients with a newly established assay].
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
June 2008
Objective: To quantitatively detect hepatitis B virus covalently closed circular DNA (HBV cccDNA) in sera of chronic hepatitis B patients with a newly established assay.
Methods: Primers and probe were designed in highly conservative region of HBV DNA. DNA was extracted from 175 sera samples of chronic hepatitis B patients, and was treated with plasmid-Safe-ATP-dependent Dnase(PSAD) to eliminate the relaxed circular DNA (rcDNA).
[Multiple-site analysis of HBV drug-resistant mutations in 340 patients with chronic hepatitis B].
Zhonghua Gan Zang Bing Za Zhi
October 2008
Article Synopsis
- The study aimed to analyze HBV drug-resistant mutations associated with nucleos(t)ide analogues in 340 patients with chronic hepatitis B.
- Researchers used serum HBV DNA extraction, nested PCR, and direct sequencing to identify mutations and analyze their clinical significance.
- Findings revealed drug-resistant mutations in a subset of patients on treatment, with specific mutations linked to resistance against drugs like lamivudine, adefovir, and entecavir, highlighting the importance of monitoring these mutations for effective HBV therapy planning.