The time-dependent variations of zebrafish intestine and gill after polyethylene microplastics exposure.

Microplastics (MPs) are common environmental contaminants that present a growing health concern due to their increasing presence in aquatic and human systems. However, the mechanisms behind MP effects on organisms are unclear. In this study, zebrafish (Danio rerio) were used as an in vivo model to investigate the potential risks and molecular mechanisms of the toxic effects of polyethylene MPs (45-53 μm).

[Expression and role of Pim1 in cultured cortical neurons with oxygen-glucose deprivation/reoxygen injury].

Objective: To study the expression and effect of Pim1 in primary cortical neurons after hypoxic-ischemic injury.

Methods: Cortical neurons were isolated from 1-day-old C57BL/6 mice and cultured in neurobasal medium. On the 8th day of neuron culture, cells were subjected to oxygen-glucose deprivation/reoxygen (OGD/R) treatment to mimic in vivo hypoxic injury of neurons.

[Effect of irisin on hypoxic-ischemic brain damage in neonatal rats].

Objective: To study the effect and mechanism of action of irisin on hypoxic-ischemic brain damage in neonatal rats.

Methods: A total of 248 7-day-old Sprague-Dawley rats were randomly divided into a sham-operation group, a model group, and low- and high-dose irisin intervention groups (n=62 each). The rats in the model and irisin intervention groups were given hypoxic treatment after right common carotid artery ligation to establish a model of hypoxic-ischemic brain damage.

[Investigation of Lentiviral Vectors Based Integrin β8 RNAi System in Neonatal Rats' Brain].

Objective: To construct lentiviral vectors expressing pSicoR- shRNA and evaluate its efficiency of RNA interference in neonatal rats' brain.

Methods: Plasmid vectors pSicoR- shRNA and pSicoR-control,as well as lentiviral packaging system and were amplified respectively and plasmid DNA was identified by restriction enzyme digestion. Lentiviral packaging system and expressing vector pSicoR- shRNA/pSicoR-control were co-transfected into packaging cell line 293T.

[Expression rhythm of autophagic gene in neurons of neonatal rats with hypoxia/ischemia and its regulatory mechanism].

Objective: To investigate the expression of autophagic gene and circadian gene in the neurons of neonatal rats after hypoxic-ischemic brain damage and the mechanism of nerve injury induced by hypoxia/ischemia.

Methods: Twelve Sprague-Dawley (SD) rats were randomly divided into hypoxic-ischemic (HI) group and sham-operation group, with 6 rats in each group. Ligation of the right common carotid artery and hypoxic treatment were performed to establish a model of hypoxic-ischemic brain damage.

Association of maternal hypertensive disorders with retinopathy of prematurity: A systematic review and meta-analysis.

Backgroud: The role of maternal hypertensive disorders in pregnancy (HDP) in the development of retinopathy of prematurity (ROP) is unclear.

Methods: Studies were retrieved through literature searches in PubMed, EMBASE, Web of Science and the Cochrane Library up to May 5, 2016 without language restrictions. Cohort or case-control studies that reported the association of maternal hypertensive disorders and retinopathy of prematurity were eligible.

[Effect of telomerase activation on biological behaviors of neural stem cells in rats with hypoxic-ischemic insults].

Objective: To investigate the effect of telomerase activation on biological behaviors of neural stem cells after hypoxic-ischemic insults.

Methods: The neural stem cells passaged in vitro were divided into four groups: control, oxygen-glucose deprivation (OGD), OGD+cycloastragenol (CAG) high concentration (final concentration of 25 μM), and OGD+CAG low concentration (final concentration of 10 μM). The latter three groups were subjected to OGD.

[Protective effect of histone acetylation against cortical injury in neonatal rats].

Objective: To investigate the protective effect of histone acetylation against hypoxic-ischemic cortical injury in neonatal rats.

Methods: A total of 90 neonatal rats aged 3 days were divided into three groups: sham-operation, cortical injury model, and sodium butyrate (a histone deacetylase inhibitor) treatment. The rats in the model and the sodium butyrate treatment groups were intraperitoneally injected with lipopolysaccharide (0.

[The neuroprotective role of exogenous TERT gene in neonatal rats with hypoxic-ischemic brain damage].

Objective: To study the effect of telomerase reverse transcriptase (TERT) on cell apoptosis in neonatal rat brains after hypoxic-ischemic brain injury (HIBD).

Methods: A total of 72 neonatal rats were divided into sham, vehicle, HIBD and TERT groups. HIBD was induced by Rice method in the later three groups.

[Long non-coding RNAs and hypoxic-ischemic brain damage].

Long non-coding RNAs (lncRNAs) are transcripts with a complex structure and a length of >200 nt which are unable to encode proteins. The lncRNAs interact with DNA, mRNA, and proteins and regulate gene expression through various mechanisms, thus participating in the regulation of various biological processes. Studies have shown that lncRNAs play important roles in neural development and the pathogenesis of diseases.

[Effects of PINK1 gene on cell apoptosis and cell autophagy in neonatal mice with hypoxic-ischemic brain damage].

Objective: To study the effect of PINK1 (phosphatase and tensin homolog deleted on chromosome ten induced putative kinase 1) gene on cell apoptosis and cell autophagy in neonatal mice with hypoxic-ischemic brain damage (HIBD).

Methods: Seventy-two wild-type C57BL/6 mice and 72 PINK1 gene knockout neonatal C57BL/6 mice were randomly divided into four groups: sham-operated wild-type (SWT), HIBD model wild-type (MWT), sham-operated knockout (SKO) and HIBD model knockout (MKO). HIBD model was prepared by low oxygen exposure for 2.

[Role of STAT3 signaling pathway in hypoxic-ischemic brain damage of neonatal rats].

Objective: To study the role and mechanisms of STAT3 signaling pathway in hypoxic-ischemic brain damage (HIBD) of neonatal rats.

Methods: Eighty 7-day-old Sprague-Dawley rats were randomly divided into two groups: HI and sham-operated (n=40 each). The rats in the HI group were subjected to right carotid artery ligation and subsequent hypoxia exposure (8% O2) for 2.

[Role of long non-coding RNA BC088414 in hypoxic-ischemic injury of neural cells].

Objective: To investigate the role of long non-coding RNA (lncRNA) BC088414 in hypoxic-ischemic injury of neural cells.

Methods: Rat adrenal pheochromocytoma (PC12) cells were divided into four groups: normoxic, oxygen glucose deprivation (OGD), siRNA-normoxic (siRNA group) and siRNA-OGD (n=3 each). Cells were incubated in glucose-free and serum-free DMEM medium under the conditions of 37℃ and 1% O2+99% N2/CO2 for 6 hours to establish an in vitro hypoxic-ischemic model.

Regulation of autophagy by the nuclear factor κB signaling pathway in the hippocampus of rats with sepsis.

Background: Sepsis with brain dysfunction has contributed to an increase risk of morbidity and mortality. In its pathophysiology, both autophagy and nuclear factor κB (NF-κB) have been suggested to play important roles. Based on the fact that crosstalk between autophagy and NF-κB, two stress-response signaling pathways, has been detected in other pathophysiological processes, this study was undertaken to explore the process of autophagy in the hippocampus of septic rats and the role NF-κB plays in the regulation of autophagy during the process.

[Effects of dimethyloxalyl glycine on hypoxic-ischemic brain damage in newborn rats].

Objective: To investigate the effects of dimethyloxalyl glycine on hypoxic-ischemic brain damage in newborn rats.

Methods: Forty eight postnatal day 10 SD rats were divided into 3 groups, including sham surgery group, hypoxic-ischemic group and DMOG treated group. The brain tissues were collected at 4, 8, 24 and 72 hours after the hypoxic-ischemic treatment.

[The studies of GLUT1 expression and neuron apoptosis in neonatal hypoxic-ischemia brain damage rat model].

Objective: To investigate the relationship between the expression of glucose transporter protein 1 and the apoptosis of neuron during hypoxic-ischemia brain damage in neonatal rats.

Methods: Total 120 10-day old SD rats were divided into normal group, sham control group and hypoxic-ischemia (HI) group. In HI group, hypoxic-ischemia brain damage (HIBD) were generated according to Rice-Vannucci method, brain tissues were harvested at 2, 8, 24, 48 and 72 h after HI.

[Preparation and application of biotinylated anti-AIB1 moniclonal antibody].

Objective: To cross-link the McAb 1A2E1 to BNHS reagents and apply this biotinylated McAb to detect AIB1 antigen of target cells.

Methods: The McAb 1A2E1 was mixed with BNHS reagents to generate Biotin-1A2E1. The competitive inhibition ELISA and immunocytochemical method were applied to detect the biologic activity of antibody.

[Hypoxia-inducible factor-1alpha: a promising target for tumor therapy].

Hypoxia-inducible factor-1alpha (HIF-1alpha), a nuclear transcriptional factor, is constitutively expressed in mammalian cells under hypoxia, which contributes a lot to the regulation of internal O2 homeostasis. Micro-environmental hypoxia is a common feature of solid tumors. Under the stress of hypoxia, HIF-1alpha is accumulated and activated, which leads to activation of a vast array of downstream genes that contribute to tumor O2 homeostasis and energy metabolic equilibrium.

[Effect of dominant negative HIF-1alpha (dn HIF-1alpha) on biological characteristics of uterine cervix cancer cells].

Objective: To explore the effect of dominant negative HIF-1alpha (dn HIF-1alpha) on biological characteristics of uterine cervix cancer cell SiHa and elucidate the related mechanism.

Methods: pcDNA3. 1-dn HIF-1alpha was transfected into SiHa cells.

[Effect of HIF-1alpha on the proliferation and apoptosis of uterine cervix cancer SiHa cells].

Objective: To explore the effect of HIF-1alpha on the proliferation and apoptosis of SiHa cells in hypoxia, as well as the role of HIF-1alpha in the development of uterine cervix cancer.

Methods: The eukaryotic expression vector containing full length HIF-1alpha (pcDNA3. 1-full length HIF-1alpha) was transfected into SiHa cell, the mock plasmid (pcDNA3.

[Effect of aurora A on carcinogenesis of human prostate cancer].

Objective: To investigate whether Aurora A is involved in prostate cancer carcinogenesis.

Methods: The expressions of Aurora A mRNA and protein in prostate cancer tissue and cell line were measured using RT-PCR, immunohistochemistry and Western blot analyses. Meanwhile, A DNAzyme targeting at Aurora A mRNA was performed to detect the inhibition effect of Aurora A in regulating the growth of prostate cancer cell line PC3.

[Effect of Aurora A on biological phenotypes of prostate cancer cells].

Objective: To examine the effect of Aurora A on the biological phenotypes of prostate cancer cells and the role of Aurora A in the carcinogenesis of prostate cancer.

Methods: Full length Aurora A gene was cloned and transfected into the prostate cancer cell line LNCaP to construct a positive cell clone with high expression of Aurora A. The growth and proliferation of the cells was detected by MTT.

[Relationship between polymorphism of interleukin-1 and pneumoconiosis susceptibility].

Objective: To explore the relationship between the polymorphism of Interleukin-1 and the pneumoconiosis susceptibility.

Methods: Eighty patients with silicosis and 45 with coal workers' pneumoconiosis (CWP) were selected while 125 male workers, Han nationality in the same workplace as the patients were selected as the controls. Between the patients and the control, the differences of age and cumulative length of service were less than five years and two years, respectively.