One new spirocyclic lactone and one new benzopyran derivative from .

One new spirocyclic lactone, terreinlactone C (), and one new benzopyran derivative, 2,2-dimethyl-3-hydroxychroman-6-aldehyde (), were discovered from the fungus . The chemical structures of compounds and were elucidated by detailedly analyzing NMR and HRESIMS data. Compound is the first natural product with a 1-oxaspiro[4.

[Scanning of drug targets related to uterus contraction from the uterine smooth muscles by cDNA microarray].

Objective: To screen the differentially expressed gene profile from the smooth muscles in the fundus uterus at the active stage of labor, and to provide candidate genes for picking out the drug targets related to uterine contraction.

Methods: Differentially expressed genes of uterine smooth muscles in the corpus from pro and post spontaneous parturition and those induced by oxytocin,as well as those from the corpus and the lower portion spontaneous parturition,were scanned respectively by human full-length genetic cDNA microarray with 8064 probe sets. Semi-quantitative RT-PCR was applied to testify the expression of voltage dependent calcium channel-L subtype (CACNA).

[Fetal posterior cranial fossa in the second and third trimester].

Objective: To examine the normal range of the width of posterior cranial fossa (WPCF) in the second and third trimester by ultrasonography, and to investigate its relationship with fetal congenital and chromosome abnormality.

Methods: WPCF of 2484 fetus (gestational age from 14 to 41 weeks) was measured by ultrasonograph routinely, and the infants were followed up.

Results: In 2848 fetus, 2772 were normal and 76 were abnormal.

[Clinical analysis of pregnancy complicated with systemic lupus erythematosus].

Objective: To investigate the interacting effects between pregnancy and flares of systemic lupus erythematosus (SLE) and to explore the best occasion for SLE patients' conception and the management during the pregnancy.

Methods: Thirty one cases of pregnancy complicated with SLE were investigated retrospectively, among whom 18 were in remission of SLE at the beginning of conception (Group A), and the other 13 either had high-activity of the disease or were first diagnosed as SLE during the pregnancy (Group B). Various doses of prednisone were administered to control SLE.

Molecular cloning of a novel mouse testis-specific spermatogenic cell apoptosis inhibitor gene mTSARG7 as a candidate oncogene.

A novel mouse gene, mTSARG7 (GenBank accession No. AY489184), with a full cDNA length of 2279 bp and containing 12 exons and 11 introns, was cloned from a mouse expressed sequence tag (GenBank accession No. BE644543) that was significantly up-regulated in cryptorchidism.

[Effects of oxytocin, misoprostol and nimodipine on expression of L-type voltage dependent calcium channel mRNA of the uterine myometrium and left ventricular myocardial cells in late pregnant rats].

Objective: To study the effect of oxytocin, misoprostol and nimodiping on expressions of L-type voltage dependent calcium channel (VDCC-L) alpha(1) and VDCC-L beta(2) mRNA in the myometrium and left ventricular myocardial cells of the late pregnant rats.

Methods: A reverse transcription polymerase chain reaction (RT-PCR) method was used to examine and quantitate VDCC-L alpha(1) and VDCC-L beta(2) mRNA expressions in the uterine myometrium and left ventricular myocardial cells of rats, which were divided into the nature labor group, oxytocin group (oxytocin inducing labor), misoprostol group (misoprostol inducing labor) and nimodipine group (active labor at 48 hours after administering nimodipine).

Results: (1) In the uterine myometrium, the levels of VDCC-L alpha(1) mRNA expression in the nature labor, oxytocin, misoprostol and nimodipine group were 0.