Population Pharmacokinetics of Voriconazole and Optimization of Dosage Regimens Based on Monte Carlo Simulation in Patients With Liver Cirrhosis.

Because voriconazole metabolism is highly influenced by liver function, the dose regimen of voriconazole should be carefully assessed in patients with liver cirrhosis. We aimed to identify significant factors associated with plasma concentrations. Blood samples were collected from patients with liver cirrhosis who received voriconazole, and voriconazole concentrations were determined.

[Preliminary study on effective components of Tripterygium wilfordii for liver toxicity based on spectrum-effect correlation analysis].

In this paper, the spectrum-effect correlation analysis method was used to explore the main effective components of Tripterygium wilfordii for liver toxicity, and provide reference for promoting the quality control of T. wilfordii. Chinese medicine T.

High prevalence of thalassemia in migrant populations in Guangdong Province, China.

Background: The objectives of this study were to estimate the prevalence of thalassemia and to analyze the need for public health services for migrant populations in different cities in Guangdong Province, China.

Methods: A cross-sectional survey was conducted in 21 cities of Guangdong Province. Twenty-three types of a- and β-globin gene mutations were detected in a total of 14,230 pregnant women and 14,249 husbands.

[Longterm outcome after the decompressive surgery for thoracic myelopathy due to the ossification of the ligamentum flavum].

Objectives: To investigate the long-term surgical outcome of thoracic myelopathy caused by the ossification of the ligamentum flavum (OLF) and evaluate the related risk factors.

Methods: Forty-four patients who underwent decompressive laminectomy with thoracic OLF between January 1990 and December 2005 and got more than 5 years follow-up were retrospectively reviewed. Among these 44 cases, there were 29 male and 15 female whose ages at operation were 52 years averagely (27-68 years).

[The posterior surgical treatment of old tuberculous kyphosis].

Objective: To observe the results of posterior osteotomy and correction in the surgical treatment of old tuberculous kyphosis.

Methods: From June 2004 to December 2008, 31 cases of old tuberculous kyphosis with posterior osteotomy and correction technique were treated. There were 12 cases of male and 19 cases of female.

Circumspinal decompression through a single posterior incision to treat thoracolumbar disc herniation.

Background: Various surgical approaches have been successfully used in the treatment of thoracolumbar disc herniation (TLDH). Although the anterior transthoracic approach has a reputation for better visualization than the posterolateral and lateral approaches, it involves the manipulation of the thoracic and pulmonary structures. Thus, this approach is technically demanding and prone to compromising the respiratory system.

Diagnosis and treatment of ossification of the ligamentum flavum associated with dural ossification: clinical article.

Object: In this paper, the authors aimed to summarize the clinical characteristics of ossification of the ligamentum flavum (OLF) associated with dural ossification (DO) and to identify improved methods for preoperative diagnosis.

Methods: Thirty-six patients who had undergone OLF surgery between February 2005 and September 2009 were included in this retrospective study. The patients were divided into 2 groups: one that included patients with intraoperative evidence of DO and a second group that included patients without DO.

[The impact of thoracic and thoracolumbar angular kyphosis on pelvic shape and sagittal alignment].

Objective: To analyze the impact of thoracic and thoracolumbar angular kyphosis on pelvic shape and sagittal alignment.

Methods: From May 2002 to June 2010, the sagittal spino-pelvic parameters were analyzed in lateral standing radiographs of 32 patients (mean age 29.6 years) with thoracolumbar angular kyphosis.

[Clinical outcomes of surgical correction for ankylosing spondylitic kyphosis].

Objective: To evaluate the clinical outcomes of posterior surgical corrective methods for ankylosing spondylitic kyphosis.

Methods: From June 2003 to June 2008, 21 cases of ankylosing spondylitic kyphosis received posterior surgical correction. There were 17 male and 4 female, and the average age was 39.

[Combined segmental resection osteotomy with dual axial rotation correction, fixation and fusion for the treatment of severe angular kyphosis].

Objective: To design a new surgical correction and fixation technique for the treatment of severe angular kyphosis, observe the feasibility, safety and effectiveness of the technique.

Methods: From May 2004 to February 2007, 16 cases with severe kyphosis (average 90.8 degrees, range 50 degrees-130 degrees) were treated with segmental resection osteotomy, section distraction, dual axial rotation correction and instrumentation fusion technique.

[Study on the current status and relative factors on reproductive health of unmarried floating population in Huizhou City].

Objective: To understand the current status and relative factors of reproductive health (RH) on unmarried floating population.

Methods: Both quantitative and qualitative surveys were employed. Quantitative survey would include questionnaire admimistration, physic examination and laboratory test while qualitative study including focus/nominal group discussion, individual and resource persons interview.

[Lumbar interbody fusion using autologous bone marrow mesenchymal stem cell-calcium phosphate ceramic composite in rhesus monkey].

Objective: To determine the osteogenic capacity of autologous bone marrow mesenchymal stem cells (BMSCs)-calcium phosphate ceramic composites in vitro and implanted as a bone graft substitute for lumbar anterior interbody fusion in rhesus monkeys.

Methods: From March 2003 to April 2005, 9 adult rhesus monkeys underwent lumbar L(3 - 4) and L(5 - 6) discectomy and interbody fusion via an anterior retroperitoneal approach. Two fusion sites in each animal were randomly assigned to two of three treatments: autogenous tricortical iliac crest bone graft (autograft group, n = 6) or cell-free ceramic graft (ceramic group, n = 6) or BMSCs-ceramic composite graft (BMSCs group, n = 6).

[Intra-operative fluoroscopy for detecting pedicle screw violation in lumbar vertebrae by guided pin axial position].

Objectives: To develop an applicable and accurate intra-operative fluoroscopical angle to observe the placement of pedicle screw and pedicle violation.

Methods: Lateral radiographs were taken on eighty lumbar pedicles in eight cadaveric vertebral specimens to measure the sagittal pedicle angles. The pedicles of the vertebral arch were scanned by computed tomography (CT) to measure the transverse pedicle angles.

[New type spinal osteotomy with cage inserting anteriorly and closing posteriorly to correct thoracolumbar kyphosis by a single posterior approach].

Objective: To evaluate the feasibility, safety and efficacy of surgical correction of thoracolumbar kyphosis, using the new type spinal osteotomy with cage inserting into the intervertebral gap anteriorly and closing posteriorly by a single posterior approach.

Methods: Since 2003, eight consecutive patients with thoracolumbar kyphosis were treated surgically. There were 4 male and 4 female with the mean age of 35 years old (from 14 to 58 years old).

[Clinical characteristics and treatment of flexion-distraction stage I injuries in subaxial cervical spine].

Objective: To study the clinical characteristics and treatment of flexion-distraction stage I injuries in subaxial cervical spine.

Methods: Twelve cases of flexion-distraction stage I injuries with delayed symptoms, admitted in our hospital between January 1995 and December 2004, were studied retrospectively. In acute phase, all of 12 cases had neck pain and limited neck movements, neurological deficits were found in 6 of 12 cases.

Generation of mouse FANCL antibody and analysis of FANCL protein expression profile in mouse tissues.

Fanconi anemia complementation group L (FANCL) is a novel Fanconi anemia protein, which mono-ubiquitinates FANCD2 as a ubiquitin E3 ligase, and plays a crucial role in DNA damage repair and chromosome stability maintenance. FANCL is involved in the proliferation of primordial germ cells (PGC) in early embryonic stages, and may play a role in the development of germ cells by forming a novel testis-specific network with testis-specific proteins in the adult testis. FancL cDNA sequence was cloned by RT-PCR from mouse testis total RNA, and expressed in E.

[Functions of FANCL in primordial germ cell formation and Fanconi anemia].

Fanconi anemia (FA) is a rare autosomal recessive disorder characterized clinically by congenital abnormalities, progressive bone marrow failure and cancer susceptibility. Cells from individuals with Fanconi anemia manifest features of spontaneous chromosomal instability and hypersensitivity to DNA cross-linking agents such as mitomycin C. Over 11 known Fanconi anemia gene products are involved in DNA damage response pathway.

[Surgical correction of post-traumatic kyphosis of thoracolumbar spine].

Objective: To determine the effectiveness of surgical correction for post-traumatic kyphosis of thoracolumbar spine.

Methods: From 1996 to 2003, 33 consecutive patients with post-traumatic kyphosis of thoracolumbar spine were corrected surgically. The mean age was 40.

[Reoperation after surgical treatment of lumbar spondylolisthesis].

Objective: To study the causes of failure following surgical treatment of lumbar spondylolisthesis, procedures for redo surgery and the final result of reoperation.

Methods: 20 patients who had a repeat operation for lumbar spondylolisthesis were studied retrospectively. On average 24.

[Survey of health status and healthcare service demand of perimenopausal women in Guangdong].

Objective: To understand the health status and healthcare service demand of perimenopausal women in Guangdong Province so as to explore the mode and methods for implementing climacteric and senectitude healthcare.

Method: Using the method of stratified and cluster sampling and according to the geographical features of Guangdong Province, we selected 4 500 perimenopausal women aged 40-65 years from 5 relatively small cities (representatives of the urban areas) and 4 counties (representatives of the rural areas) for this survey.

Results: The average age of natural menopause was 48.

[Treatment of spinal fractures complicating ankylosing spondylitis].

Objective: To study the treatment of spinal fractures in ankylosing spondylitis.

Methods: Nineteen cases of spinal fractures complicating ankylosing spondylitis admitted in our hospital were studied retrospectively.

Results: All of 19 cases were up to the diagnosis standards of ankylosing spondylitis.

[Inducing human marrow mesenchymal stem cells into osteoblasts directionally and identification of their osteogenesis characteristics].

Objective: To study the method of inducing human marrow mesenchymal stem cells (MSCs) into osteoblasts directionally and to identify osteogenesis characteristics.

Methods: MSCs were isolated from adult marrow using density gradient separation method and were cultured in conditioned medium containing Dex 10(-8) mol/L, beta-GP 10 mmol/L, and AA 50 micrograms/ml. The MSCs attachment formed soon and passage 3 cells were chosen to check osteogenesis characteristics, including alkaline phosphatase assay with modified calcium-cobalt staining method, type I collagen assay with immunohistochemistry, osteopontin and osteonectin assay with in situ hybridization and calcium nodes assay with Von Kossa staining.

[Immortalization of human osteoblast by transferring hTERT gene].

Objective: To establish an immortalized osteoblast cell line used for basal and clinical research of orthopaedics.

Methods: Human marrow stromal cells (hMSCs) had been inducted into osteoblasts directionally, and human telomerase reverse transcriptase (hTERT) cDNA was transferred into the osteoblasts by retroviral vector pLPChTERT. The resultant stable clones reproduced successively and the expression of hTERT as well as the osteogenesis characteristics of different eras were identified.

[Genetic polymorphism of six short tandem repeat loci in the Han population in Hebei province of China].

Objective: This article reports a population genetic study on six short tandem repeat(STR) loci, D7S820, D19S253, D12S391, D5S818, D16S539 and D8S1179, in a sample of unrelated Chinese Han individuals(n=122-173) living in Hebei province.

Methods: DNA extraction from blood samples (200 in number) and multiplex amplification of the above six loci were carried out. Using denaturing polyacrylamide gel electrophoresis and silver stain, the authors investigated the distribution of allele frequencies of the six loci in Han population in Hebei province.

[Effect of simvastatin on bone morphogenetic protein-2 expression and alkaline phosphatase activity of bone marrow stromal cell].

Objective: To study the effect of simvastatin on the expression of bone morphogenetic protein-2 (BMP-2) and alkaline phosphates (ALP) activity in the primary cultured bone marrow stromal cells, and to elucidate the mechanism of the anabolic osteogenetic effect of simvastatin.

Methods: Bone marrow stromal cells in femur and tibia of adult mouse were cultured in vitro. after treated with different concentrations of simvastatin (0, 0.