Prediction of post-hepatectomy liver failure and long-term prognosis after curative resection of hepatocellular carcinoma using liver stiffness measurement.

Background And Study Aims: Post-hepatectomy liver failure (PHLF) is the main cause of perioperative death after hepatocellular carcinoma (HCC) resection. PHLF occurrence is related to both the hepatectomy volume and the degree of cirrhosis. Accurate preoperative assessment of the degree of cirrhosis may aid in reducing the incidence of PHLF.

Short interfering RNA directed against the GOLPH3 gene enhances the effect of chemotherapy against oral squamous cell carcinoma by regulating Caspase3, Bcl2 and cytochrome-c expression.

Growing evidence reported that Golgi phosphoprotein 3 (GOLPH3) was involved in the progression of several human cancers. To determine whether knockout of GOLPH3 enhances the effect of Chemotherapy against cell growth of oral squamous cell carcinoma in vitro. OSCC cells were transfected with Golph3 plasmid, Golph3-RNAi and the relative control plasmids.

[The citation analysis of Chinese Journal of Internal Medicine from 2005 to 2011].

Objective: To evaluate the academic level of Chinese Journal of Internal Medicine by analyzing its citation status by using bibliometrics method.

Methods: The distribution of articles published in Chinese Journal of Internal Medicine from January, 2005 to December, 2011 indexed by Chinese Science Citation Database (CSCD) was analyzed.

Results: A total of 2809 articles were published in Chinese Journal of Internal Medicine between January,2005 and December, 2011.

[An analysis of impact factor of Chinese Journal of Internal Medicine from 2008 to 2010].

Objective: To analyze the articles and citation published in the Chinese Journal of Internal Medicine from 2008 to 2010, in order to investigate the influence factors of impact factor (IF).

Methods: All articles published in the Chinese Journal of Internal Medicine covered by Chinese Medical Citation Index(CMCI) from 2008 to 2010 were searched and downloaded. Some article related attributions were manual added and their influences to IF were analyzed.

[Study on clinical manifestation, genotype and genetic characteristics of two Kennedy disease pedigrees].

Objective: To investigate the clinical manifestations, genotypes, and genetic characteristics of two pedigrees with Kennedy disease.

Methods: The clinical data of the patients from two Kennedy disease families were collected. The numbers of trinucleotide CAG repeats in exon 1 of the androgen receptor gene were determined by DNA sequencing and repeat fragment analysis.

[Polymorphism of DXS15, CA13, CA22 loci in Guangdong normal population].

The aim of this study was to investigate the polymorphism of microsatellite repeats DXS15, CA13, CA22 tightly linked to FVIII gene in Guangdong population and its practical value in genetic diagnosis for hemophilia A. The polymerase chain reaction (PCR) and capillary electrophoresis (CE) methods were adopted to test the variability of the 3 microsatellite repeat in Guangdong females, including 111 females, 222 X chromosomes for detecting DXS15 polymorphism; 87 females, 174X chromosomes for detecting CA13 polymorphism; 94 females, 188 X chromosomes for detecting CA22 polymorphism. The results indicated that 11 alleles corresponding to DXS15 were found at this locus with size ranging from 140 to 160 bp.

B16-F10 melanoma cells and cell culture supernatant enhance angiogenesis in mouse ischemic limb.

Generation of therapeutic angiogenesis to enhance vascularization in the ischemic tissues is a method for treating ischemic tissues in atherosclerotic cardiovascular artery disease. The chemokine stromal cell-derived factor-1 (SDF-1) and its receptor (CXC chemokine receptor 4, CXCR4) play a critical role in the process of post-natal neovascularization. The SDF-1-CXCR4 axis is a potential mechanism for the treatment of ischemic limb.

[Research on the procedure for recovery and species identification of Legionella from surface environmental water].

Objective: To establish a set of procedure for recovery and species identification of Legionella from the surface environmental water.

Methods: Forty-four water samples were collected in eight parks of Guangzhou city from August to November in 2006. The bacteriologic examination was performed by cultivation on BCYEalpha plate, and 108 presumptive Legionella colonies were picked and their homogeneous relationship was analyzed by using an amplified fragment length polymorphism (AFLP) method.

[Polymorphisms of human papillomavirus type 81 L1 gene in patients with cervical lesions in Guangdong].

Objective: To investigate the positivity rates and genetic polymorphism of human papillomavirus (HPV) type 81 (HPV81) L1 gene in patients with cervical cancer in Guangdong.

Methods: HPV L1 genes fragment in 1200 positive cervical samples detected with HC-II for high-risk HPV DNA were amplified by HPV-specific PCR with consensus primers, and the L1 genes of HPV81 were sequenced for mutation analysis.

Results: Four HPV81-positive cases were found in the total of 1200 cases for the positivity rate of 0.

[Expression of Class I integrase gene in Acinetobacter baumannii and drug-resistance].

Objective: To explore the expression level of class I integrase (intI 1) mRNA in Acinetobacter baumannii from biofilm cells and planktonic cultured cells ,and to analyze the drug-resistance of Class I integron positive strains.

Methods: Acinetobacter baumannii were collected from hospitals,and Class I integron strains were screened by gene amplification. Total RNA of Class I integron positive strains was extracted, and the intI1 mRNA expression in the bioflim cells and planktonic cultured cells was measured by RT-PCR.

[Beta-thalassemia major caused by compound heterozygosity for +40 to +43(-AAAC), IVS-2-654 (C to T) and codon 41/42 (-TCTT)].

Objective: To report the analysis of a rare beta-thalassemia ternary heterozygote [+40 to +43(-AAAC)*CD41/42(-TTCT)*IVS-2-654] causing beta-thalassemia major in a Chinese.

Methods: Using PCR-ASO probe hybridization analysis to scan 17 known types of beta-thalassemia mutations, and gene cloning and DNA sequencing to identify the underlying causative mutation.

Results: Reverse dot blot (RDB) analysis showed that the patient's beta-globin gene had three mutations: +40 to +43(-AAAC), CD41/42(-TCTT) and IVS-2-654(C to T).

[Occurrence, distribution and fate of antibiotics in sewage treatment plants].

Occurrence and distribution of eight selected antibiotics were screened at four sewage treatment plants (STPs). Individual concentrations in raw influent and final effluent from the four STPs ranged from 16 to 1987 ng x L(-1) and from 16 to 2054 ng x L(-1), respectively. The other analytes were only detected in a few samples with lower concentrations.

WITHDRAWN: Effect of monogalactosyldiacylglycerol on the interactions between light-harvesting complexes II and photosystem II core complexes reconstituted into liposomes constituted of thylakoid lipids.

This article has been withdrawn consistent with Elsevier Policy on Article Withdrawal (http://www.elsevier.com/locate/withdrawalpolicy).

[Surgical treatment for lumbar discogenic low back pain].

Objective: To explore the curative effect of surgical treatment for lumbar discogenic low back pain.

Methods: Forty-two patients with lumbar discogenic low back pain were treated with disc excision and lumbar interbody fusion: 12 of them were treated with posterior lumbar interbody fusion (PLIF), 30 with afront lumbar interbody fusion (ALIF). The clinical results were evaluated by pre- and post-operative visual analogue scale (VAS), and the fusion rates of the lumbosacral spine were evaluated by X-ray.