[The relationship between glutathione S-transferase M1 genotypes and lipid peroxidation in asbestos workers].

Objective: To study the relationship between glutathione S-transferase M1 (GSTM1) genotypes and lipid peroxidation of asbestos workers.

Methods: 94 asbestos workers and 51 controls were selected as subjects. The general information, occupational history and individual habits were collected by questionnaires in all participants.

The contrast of immunohistochemical studies of myocardial fibrinogen and myoglobin in early myocardial ischemia in rats.

In this study, an animal model of early myocardial ischemia (EMI) was established by ligating the left anterior descending coronary artery of rats. The experimental animals were divided into five groups according to different intervals of MI (15, 30min, 1, 2, and 3h) and one control group. Tissues from the apex of the myocardium and the adjacent myocardium were taken for paraffin sections, followed by hematoxylin-eosin and streptavidin-biotin-peroxidase complex (SABC) staining.

[Studies on the phenolic acid constituents from Chinese medicine "sheng-ma", rhizome of Cimicifuga foetida L].

Aim: To look for new active constituents from Chinese medicine "Sheng-ma", rhizome of Cimicifuga foetida L.

Methods: The compounds were separated and purified by chromatography on silica gel and Sephadex LH-20. Their structures were determined by spectral analysis and chemical reaction.

[Studies on the chemical constituents of the aerial part of Cimicifuga foetida L].

Aim: To look for new active constituents from the aerial part of Cimicifuga foetida L.

Methods: Various column chromatographic techniques were used for the isolation and purification of the principles. The structures were elucidated on the basis of spectral data and chemical evidences.

Adaptor and clathrin exchange at the plasma membrane and trans-Golgi network.

We previously demonstrated, using fluorescence recovery after photobleaching, that clathrin in clathrin-coated pits at the plasma membrane exchanges with free clathrin in the cytosol, suggesting that clathrin-coated pits are dynamic structures. We now investigated whether clathrin at the trans-Golgi network as well as the clathrin adaptors AP2 and AP1 in clathrin-coated pits at the plasma membrane and trans-Golgi network, respectively, also exchange with free proteins in the cytosol. We found that when the budding of clathrin-coated vesicle is blocked without significantly affecting the structure of clathrin-coated pits, both clathrin and AP2 at the plasma membrane and clathrin and AP1 at the trans-Golgi network exchange rapidly with free proteins in the cytosol.

[Studies on the triterpenoid constituents from the aerial part of Cimicifuga foetida L].

Aim: To look for new active constituents from the aerial part of Cimicifuga foetida L.

Methods: Various column chromatographic techniques were used for the isolation and purification of the ingredients. The structure were elucidated on the basis of spectral evidences and chemical reaction.

Protection of cap-dependent protein synthesis in vivo and in vitro with an eIF4G-1 variant highly resistant to cleavage by Coxsackievirus 2A protease.

The shutoff of host protein synthesis by certain picornaviruses is mediated, at least in part, by proteolytic cleavage of eIF4G-1. Previously, we developed a cleavage site variant of eIF4G-1, termed eIF4G-1(SM), that was 100-fold more resistant to in vitro cleavage by Coxsackievirus 2A protease (2A(Pro)) than wild-type eIF4G-1 (eIF4G-1(WT)), but it was still digested at high protease concentrations. Here we identified a secondary cleavage site upstream of the primary site.

[Effects of exposure to asbestos on plasma activity of glutathione S-transferases].

Objective: To understand the effects of exposure to asbestos and GSTM1 genotypes on plasma activity of glutathione S-transferases (GSTs).

Methods: Ninety-four workers exposed to asbestos and 51 controls were selected, and their general information, occupational history and personal behavior were collected by questionnaire. Venous blood specimen was collected from each of them and plasma was separated for detection of GSTs activity and lymphocytes for DNA extraction and GSTM1 genotyping.