Sequence and organization of the complete mitochondrial genome of Hebius vibakari ruthveni from China.

The mitochondrial genome sequence of Hebius vibakari ruthveni is analyzed and presented for the public for the first time. The genome was 17,259 bp in length and contained 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes, and 2 control regions. The overall base composition is A (33.

Sequence and organization of the complete mitochondrial genome of the Ussuri mamushi (Gloydius ussuriensis).

The mitochondrial genome sequence of Ussuri mamushi is analyzed and presented publically for the first time. The genome is 17,208 bp in length and contains 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes and 2 control regions. The overall base composition is A (32.

The complete mitochondrial genome of Cherskii's sculpin (Cottus czerskii) (Scorpaeniformes: Cottidae).

The complete mitogenome sequence of Cherskii's sculpin (Cottus czerskii) is determined using long PCR. The genome is 16,534 bp in length and contains 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes, and a control region. The overall base composition of the heavy strand is A (26.

[Silencing of Pin1 suppresses hyperoxia-induced apoptosis of A549 cells].

Objective: To explore the effect of silence of Pin1 expression on hyperoxia-induced apoptosis in alveolar epithelial cells A549.

Methods: A549 cells were divided into four groups: control, hyperoxia, negative lentivirus and Pin1-shRNA hyperoxia. The hyperoxia group was exposed to a mixture of 95%O2 and 5%CO2 for 10 minutes.

The complete mitochondrial genome of Gloydius intermedius (Squamata: Viperidae: Crotalinae) from China.

The mitochondrial genome sequence of Gloydius intermedius is analyzed and presented for the first time. The genome was 17, 226 bp in length and contained 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes and 2 control region. The overall base composition was A (32.

The complete mitochondrial genome of Amur bitterling (Rhodeus sericeus) from China.

The complete mitogenome sequence of Amur bitterling (Rhodeus sericeus) was determined using long PCR. The genome was 16,581 bp in length and contained 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes, 1 origin of L strand replication and 1 control region. The overall base composition of the heavy strand was A (28.

expanded human circulating Vδ1 γδT cells exhibit favorable therapeutic potential for colon cancer.

Gamma delta T (γδT) cells are innate-like lymphocytes with strong, MHC-unrestricted cytotoxicity against cancer cells and show a promising prospect in adoptive cellular immunotherapy for various malignancies. However, the clinical outcome of commonly used Vγ9Vδ2 γδT (Vδ2 T) cells in adoptive immunotherapy for most solid tumors is limited. Here, we demonstrate that freshly isolated Vδ1 γδT (Vδ1 T) cells from human peripheral blood (PB) exhibit more potent cytotoxicity against adherent and sphere-forming human colon cancer cells than Vδ2 T cells .

[Resveratrol inhibits hyperxia-induced cell apoptosis through up-regulating SIRT1 expression in HPAECs].

Objective: To investigate the protection effect of sirtuin 1 (SIRT1) agonist resveratrol (Res) against the apoptosis of human pulmonary alveolar epithelial cells (HPAECs) induced by hyperxia.

Methods: The HPAECs in vitro were randomly divided into three groups: control group, hyperxia group, Res group. After 24-hour culture, the expressions of caspase-9, X-linked inhibitor of apoptosis protein (XIAP), SIRT1 proteins were measured by SP immunohistochemistry.

Efficacy of anti-tumor necrosis factor therapy for extra-articular manifestations in patients with ankylosing spondylitis: a meta-analysis.

Background: We performed a meta-analysis to evaluate the effect of anti-tumor necrosis factor (TNF) therapy on the frequency of extra-articular manifestations (EAMs) in patients with ankylosing spondylitis (AS).

Methods: We searched with the terms 'ankylosing spondylitis', 'infliximab', 'etanercept', 'adalimumab', 'golimumab', 'certolizumab', 'TNF inhibitor/blocker/antagonists' or 'anti-TNF' on MEDLINE, EMBASE and Cochrane Library for randomized controlled trials (RCTs) of ≥ 12 weeks with parallel or crossover design of TNF inhibitor versus placebo to treat uveitis, inflammatory bowel disease (IBD) and/or psoriasis of AS, published before February 2014.

Results: We found 8 RCTs that fit our criteria.

Sequencing and analysis of the complete mitochondrial genome of tundra shrew (Sorex tundrensis) from China.

The complete mitogenome sequence of tundra shrew (Sorex tundrensis) was determined using long PCR. The genome was 17,444 bp in length and contained 13 protein-coding genes, 2 ribosomal RNA genes, 23 transfer RNA genes, 1 origin of L strand replication and 1 control region. The overall base composition of the heavy strand is A (32.

Sequencing and analysis of the complete mitochondrial genome of Gloydius saxatilis (Squamata: Viperidae: Crotalinae).

The mitochondrial genome sequence of Gloydius saxatilis is analyzed and presented for the public for the first time. The genome was 17,218 bp in length and contained 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes and 2 control regions. The overall base composition was A (32.

Sequencing and analysis of the complete mitochondrial genome of Elaphe davidi (Squamata: Colubridae).

The mitochondrial genome sequence of Elaphe davidi is analyzed and presented for the first time in this work. The genome was 17,117 bp in length and contained 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes and 2 control region. The overall base composition is A (35.

A versatile platform of 2-(3,4-dihydroxyphenyl) pyrrolidine grafted graphene for preparation of various graphene-derived materials.

Covalent functionalization has proven an effective solution for graphene to realize its revolutionary potential in real applications, whereas the platform strategy (a reactive graphene-based material acting as the platform to undergo post-reactions for generation of various graphene-derived materials) is an attractive option to execute efficiently such a task. This contribution demonstrates that 2-(3,4-dihydroxyphenyl) pyrrolidine (DHPP) grafted graphene, G-OH, is a competent platform. Four typical but not exclusive graphene-derived materials have been prepared from G-OH by using the chemical virtue of each DHPP unit having three categories totaling six reactive sites.

Biochemical and molecular changes associated with heteroxylan biosynthesis in Neolamarckia cadamba (Rubiaceae) during xylogenesis.

Wood, derived from plant secondary growth, is a commercially important material. Both cellulose and lignin assembly have been well studied during wood formation (xylogenesis), but heteroxylan biosynthesis is less well defined. Elucidation of the heteroxylan biosynthetic pathway is crucial to understand the mechanism of wood formation.

Actinotalea suaedae sp. nov., isolated from the halophyte Suaeda physophora in Xinjiang, Northwest China.

A Gram-stain-positive, aerobic, non-motile, coryneform bacterium, designated strain EGI 60002(T), was isolated from the halophyte Suaeda physophora. Cells were coryneform shaped and polymorphic. Phylogenetic analysis based on 16S rRNA gene sequences showed that the new isolate was closely related to Actinotalea ferrariae CF5-4(T) (95.

The complete mitochondrial genome of Vespertilio sinensis from China.

The complete mitogenome sequence of Vespertilio sinensis was determined using long PCR. The genome was 17,146 bp in length and contained 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes and 1 control region. The overall base composition of the heavy strand is A (32.

Oxidative coupling of benzylamines to imines by molecular oxygen catalyzed by cobalt(II) β-tetrakis(trifluoromethyl)-meso-tetraphenylporphyrin.

Oxidative coupling of benzylamines to imines by molecular oxygen is efficiently realized in the presence of very low catalyst loadings of Co(II) β-tetrakis(trifluoromethyl)-meso-tetraphenylporphyrin. Due to the effect of four β-CF3 groups, the catalyst shows good selectivity and very high turnover number. The reaction is easily scaled up and may provide a convenient way to prepare many imines in large scale.

Lignin plays a negative role in the biochemical process for producing lignocellulosic biofuels.

A biochemical platform holds the most promising route toward lignocellulosic biofuels, in which polysaccharides are hydrolyzed by cellulase enzymes into simple sugars and fermented to ethanol by microbes. However, these polysaccharides are cross-linked in the plant cell walls with the hydrophobic network of lignin that physically impedes enzymatic deconstruction. A thermochemical pretreatment process is often required to remove or delocalize lignin, which may also generate inhibitors that hamper enzymatic hydrolysis and fermentation.

A solid-phase assay for studying direct binding of progranulin to TNFR and progranulin antagonism of TNF/TNFR interactions.

The discovery that TNF receptors (TNFR) serve as the binding receptors for progranulin (PGRN) reveals the significant role of PGRN in inflammatory and autoimmune diseases, including inflammatory arthritis. Herein we describe a simple, antibody-free analytical assay, i.e.

MicroRNA expression profiles of peripheral blood mononuclear cells in patients with systemic lupus erythematosus.

Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by the presence of autoantibodies against numerous self-antigens. Evidence underlines the importance of microRNAs (miRNAs) in the pathogenesis of SLE, but the exact etiology of this disease is unknown. Therefore, this study was conducted to explore regulation of abnormal miRNAs in SLE using microarray analysis.

Hydrogen-bond-directed formal [5 + 1] annulations of oxindoles with ester-linked bisenones: facile access to chiral spirooxindole δ-lactones.

A novel bifunctional thiourea catalyzed formal [5 + 1] cycloaddition of oxindoles and ester-linked bisenones was successfully developed. This strategy involves two sequential Michael additions, leading to spirooxindole δ-lactones with three contiguous stereocenters including an all-carbon quaternary center with high diastereo- and enantioselectivites. In addition, a remarkable N-substituent effect was observed on the reactivity and selectivity.

[The interference effect of lentiviral mediated shRNA on the expression of Pin 1 in A549 cells].

Objective: To construct a Pin1shRNA lentiviral vector and transfect stably A549 cells.

Methods: The peptidyl-proplyl cis-trans isomerase 1(Pin1) gene sequences from GenBank were cloned into the pLenR-GPH-shRNA lentiviral vector. The pLenR-GPH-shRNA and lentivector pakaging plasmid mix were cotransfected into human embryonic kidney cells HEK293T to package lentiviral particles.

Applications of capillary electrophoresis in characterizing recombinant protein therapeutics.

The use of recombinant protein for therapeutic applications has increased significantly in the last three decades. The heterogeneity of these proteins, often caused by the complex biosynthesis pathways and the subsequent PTMs, poses a challenge for drug characterization to ensure its safety, quality, integrity, and efficacy. CE, with its simple instrumentation, superior separation efficiency, small sample consumption, and short analysis time, is a well-suited analytical tool for therapeutic protein characterization.

Progranulin directly binds to the CRD2 and CRD3 of TNFR extracellular domains.

We previously reported that PGRN directly bound to TNF receptors (TNFR) in vitro and in chondrocytes (Tang, et al., Science, 2011). Here we report that PGRN also associated with TNFR in splenocytes, and inhibited the binding of TNFα to immune cells.