Salmonella enterica virulence databases and bioinformatic analysis tools development.

Salmonella enterica, a prominent foodborne pathogen, contributes significantly to global foodborne illnesses annually. This species exhibits significant genetic diversity, potentially impacting its infectivity, disease severity, and antimicrobial resistance. Whole genome sequencing (WGS) offers comprehensive genetic insights that can be utilized for virulence assessment.

Coupling antitoxins and blue/white screening with /resolvase mutation as a strategy for spp. plasmid curing.

Unlabelled: Despite the dissemination of multidrug resistance plasmids, including those carrying virulence genes in spp., efficient plasmid curing tools are lacking. Plasmid partitioning and multimer resolution systems are attractive targets for plasmid cure.

The acquired gene cluster in mediates resistance to copper.

The pervasive environmental metal contamination has led to selection of heavy-metal resistance genes in bacteria. The and clusters are located on a mobile genetic element and linked to heavy-metal resistance. These clusters have been found in serovars isolated from human clinical cases and foods of animal origin.

Fluvial fan sedimentary characteristics of distributive fluvial system.

The characteristics of fan sediment in the distributive fluvial system are evaluated based on a thorough analysis of modern silt in the Guertu river distributive fluvial system, as well as data obtained from UAV aerial photography and satellite remote sensing. The Guertu River Distributive Fluvial System (DFS) is classified into three stages, namely "proximal," "middle," and "distal," based on the examination of river morphology, sediment variations, sedimentary attributes, and other relevant aspects throughout different sections of its tributaries, from the source to the mouth. At the upstream section of the downstream fining sequence, the slope is the steepest and the hydrodynamic conditions are intense, resulting in the formation of a predominantly big gravel braided river.

Genetic relatedness and virulence potential of Schwarzengrund strains with or without an IncFIB-IncFIC(FII) fusion plasmid isolated from food and clinical sources.

A total of 55 food and clinical . Schwarzengrund isolates were assayed for plasmid content, among which an IncFIB-IncFIC(FII) fusion plasmid, conferring streptomycin resistance, was detected in 17 isolates. Among the 17 isolates, 9 were food isolates primarily collected from poultry meat, and 8 clinical isolates collected from stool, urine, and gallbladder.

Evaluation of the value of combined detection of tumor markers CA724, carcinoembryonic antigen, CA242, and CA19-9 in gastric cancer.

Background: Gastric cancer is a global health concern that poses a significant threat to human well-being.

Aim: To detecting serum changes in carcinoembryonic antigen (CEA), carbohydrate antigens (CA) 724, CA242, and CA19-9 expression among patients with gastric cancer.

Methods: Eighty patients diagnosed with gastric cancer between January 2020 and January 2023 were included in the observation group, while 80 patients with benign gastric diseases were included in the control group.

The spread of pESI-mediated extended-spectrum cephalosporin resistance in Salmonella serovars-Infantis, Senftenberg, and Alachua isolated from food animal sources in the United States.

The goal of this study is to investigate the origin, prevalence, and evolution of the pESI megaplasmid in Salmonella isolated from animals, foods, and humans. We queried 510,097 Salmonella genomes under the National Center for Biotechnology Information (NCBI) Pathogen Detection (PD) database for the presence of potential sequences containing the pESI plasmid in animal, food, and environmental sources. The presence of the pESI megaplasmid was confirmed by using seven plasmid-specific markers (rdA, pilL, SogS, TrbA, ipf, ipr2 and IncFIB(pN55391)).

Infection biology of .

is the leading cause of bacterial foodborne illness in the USA, with an estimated 95% of salmonellosis cases due to the consumption of contaminated food products. can cause several different disease syndromes, with the most common being gastroenteritis, followed by bacteremia and typhoid fever. Among the over 2,600 currently identified serotypes/serovars, some are mostly host-restricted and host-adapted, while the majority of serotypes can infect a broader range of host species and are associated with causing both livestock and human disease.

Complete genome sequences of 17 serovar Schwarzengrund isolates carrying an IncFIB-IncFIC (FII) fusion plasmid.

Seventeen serovar Schwarzengrund isolates from chicken ( = 9) and clinical samples including stool ( = 6), urine ( = 1), and gallbladder ( = 1) were sequenced and found to carry an IncFIB-IncFIC (FII) fusion plasmid of approximately 145 Kb. This information provides reference genomic data for comparative studies of . Schwarzengrund pathogenicity and plasmid genetics.

[A dual-crosslinked injectable hydrogel derived from muscular decellularized matrix promoting myoblasts proliferation and myogenic differentiation].

Objective: To investigate the feasibility of a dual-crosslinked injectable hydrogel derived from acellular musclar matrix (AMM) for promoting myoblasts proliferation and myogenic differentiation.

Methods: Firstly, hyaluronic acid was oxidized with NaIO and methylated to prepare methacrylamidated oxidized hyaluronic acid (MOHA). Then, AMM obtained by washing enzymatically treated muscle tissue was aminolyzed to prepare aminated AMM (AAMM).

Genomic analysis of azithromycin-resistant from food animals at slaughter and processing, and retail meats, 2011-2021, United States.

Macrolides of different ring sizes are critically important antimicrobials for human medicine and veterinary medicine, though the widely used 15-membered ring azithromycin in humans is not approved for use in veterinary medicine. We document here the emergence of azithromycin-resistant among the NARMS culture collections between 2011 and 2021 in food animals and retail meats, some with co-resistance to ceftriaxone or decreased susceptibility to ciprofloxacin. We also provide insights into the underlying genetic mechanisms and genomic contexts, including the first report of a novel combination of azithromycin resistance determinants and the characterization of multidrug-resistant plasmids.

Eco-friendly microalgae harvesting using lipid-cored particles with a comparative life-cycle assessment.

This study proposed an innovative approach using lipid-cored particles (LCPs) aimed at addressing the efficiency, cost, and environmental impact challenges in microalgae harvesting. Cetyltrimethylammonium bromide (CTAB) and chitosan (CS) were used to modify LCPs and to optimize efficiency and investigate the mechanisms of harvesting with Chlorella vulgaris. Results showed that a maximum harvesting efficiency of 97.

The role of satellite remote sensing in mitigating and adapting to global climate change.

Climate change has critical adverse impacts on human society and poses severe challenges to global sustainable development. Information on essential climate variables (ECVs) that reflects the substantial changes that have occurred on Earth is critical for assessing the influence of climate change. Satellite remote sensing (SRS) technology has led to a new era of observations and provides multiscale information on ECVs that is independent of in situ measurements and model simulations.

Dose-response relationship between multiple trace elements and risk of all-cause mortality: a prospective cohort study.

Objectives: We aimed to prospectively investigate the independent and combined relationship between trace elements concentrations [blood (selenium, manganese), serum (copper, zinc), and urine (cobalt, molybdenum, tin, strontium, iodine)] and all-cause mortality.

Methods: This study included 5,412 individuals with demographical, examination, and laboratory data from the National Health and Nutrition Examination Survey. Three statistical models, including Cox proportional hazards models, restricted cubic spline models, and Bayesian kernel machine regression (BKMR) models, were conducted to estimate the longitudinal relationship between trace elements and all-cause mortality.

Systemic inflammation indicators and risk of incident arrhythmias in 478,524 individuals: evidence from the UK Biobank cohort.

Background: The role of systemic inflammation in promoting cardiovascular diseases has attracted attention, but its correlation with various arrhythmias remains to be clarified. We aimed to comprehensively assess the association between various indicators of systemic inflammation and atrial fibrillation/flutter (AF), ventricular arrhythmia (VA), and bradyarrhythmia in the UK Biobank cohort.

Methods: After excluding ineligible participants, a total of 478,524 eligible individuals (46.

[Research of diclofenac sodium-loaded gelatin scaffold with anti-inflammatory activity for promoting cartilage regeneration].

Objective: To develop a diclofenac sodium-loaded gelatin scaffold with anti-inflammatory activity and provide a new avenue for alleviating the inflammatory response and enhancing cartilage regeneration .

Methods: Diclofenac sodium was homogeneously mixed with gelatin to prepare a diclofenac sodium-loaded porous gelatin scaffold by freeze-drying method as the experimental group, and a pristine porous gelatin scaffold was served as a control group. The general morphology of the scaffold was observed, the pore size of the scaffold was measured by scanning electron microscopy, the porosity of the scaffold was calculated by drainage method, the loading of diclofenac sodium into the gelatin scaffold was detected by fourier transform infrared spectrometer and X-ray diffraction examinations, and the release kinetics of diclofenac sodium from gelatin scaffold was tested using an release assay.

Geography shapes the genomics and antimicrobial resistance of Salmonella enterica Serovar Enteritidis isolated from humans.

Multidrug-resistant (MDR) Salmonella has been a long-standing challenge in public health and food safety. The prevalence of MDR S. Enteritidis, especially isolated from humans, in China is significantly higher than those from the U.

Icariin-loaded hydrogel with concurrent chondrogenesis and anti-inflammatory properties for promoting cartilage regeneration in a large animal model.

Currently, an effective repair method that can promote satisfactory cartilage regeneration is unavailable for cartilage damages owing to inevitable inflammatory erosion. Cartilage tissue engineering has revealed considerable treatment options for cartilage damages. Icariin (ICA) is a flavonoid component of with both chondrogenic and anti-inflammatory properties.

[Effect of xanthohumol-loaded anti-inflammatory scaffolds on cartilage regeneration in goats].

Objective: To develop an anti-inflammatory poly (lactic-co-glycolic acid) (PLGA) scaffold by loading xanthohumol, and investigate its anti-inflammatory and cartilage regeneration effects in goats.

Methods: The PLGA porous scaffolds were prepared by pore-causing agent leaching method, and then placed in xanthohumol solution for 24 hours to prepare xanthohumol-PLGA scaffolds (hereinafter referred to as drug-loaded scaffolds). The PLGA scaffolds and drug-loaded scaffolds were taken for general observation, the pore diameter of the scaffolds was measured by scanning electron microscope, the porosity was calculated by the drainage method, and the loading of xanthohumol on the scaffolds was verified by Fourier transform infrared (FTIR) spectrometer.

Genomic Diversity, Antimicrobial Resistance, and Virulence Gene Profiles of Serovar Kentucky Isolated from Humans, Food, and Animal Ceca Content Sources in the United States.

serovar Kentucky is frequently isolated from chickens and dairy cattle, but recovery from humans is comparatively low based on the U.S. National Antimicrobial Resistance Monitoring System (NARMS) reports.

Rapid migration of mainland China's coastal erosion vulnerability due to anthropogenic changes.

With the global rise in sea levels caused by climate change and frequent extreme weather processes, high-density population aggregation and human development activities to enhance coastal areas vulnerability, populations, resources, and the ecological environment are facing huge pressure. Natural coastlines are being destroyed, and increasingly serious problems, such as coastal erosion and ecological fragility, have become disasters in coastal zones. The coastal vulnerability changed by climatic variables has created a major concern at regional, national and global scales.