serovar Schwarzengrund: Distribution, Virulence, and Antimicrobial Resistance.

The global incidence of serovar Schwarzengrund has risen in recent years. This serotype has been isolated from poultry, retail meat, and other food products, leading to multiple outbreaks. Alongside the increase in infections, there are growing concerns about the increasing levels of antimicrobial resistance (AMR) among Schwarzengrund strains.

Effects of Mixing Ratio and Lactic Acid Bacteria Preparation on the Quality of Whole-Plant Quinoa and Whole-Plant Corn or Stevia Powder Mixed Silage.

Quinoa is the only single plant that can meet all the nutritional needs of human, and its potential for feed utilization has been continuously explored, becoming a prosperous industry for poverty alleviation. In order to further tap the feeding value of whole quinoa, develop quinoa as a feed substitute for conventional crops such as corn, and improve its comprehensive utilization rate, this experiment analyzed the silage quality and mycotoxin content of mixed silage of whole-plant quinoa (WPQ) with whole-plant corn (WPC) or stevia powder(SP) in different proportions, and further improved the silage quality of mixed silage by using two lactic acid bacteria preparations (Sila-Max and Sila-Mix). The quality, microbial population, and mycotoxin levels of quinoa and corn silage, as well as that of the mixed silage of quinoa and stevia, were evaluated using single-factor analysis of variance.

Visual and High-Efficiency Secretion of SARS-CoV-2 Nanobodies with .

Nanobodies have gained attention as potential therapeutic and diagnostic agents for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) due to their ability to bind and neutralize the virus. However, rapid, scalable, and robust production of nanobodies for SARS-CoV-2 remains a crucial challenge. In this study, we developed a visual and high-efficiency biomanufacturing method for nanobodies with by fusing the super-folder green fluorescent protein (sfGFP) to the N-terminus or C-terminus of the nanobody.

The Impact of the Lactation Period Gut Microbiota of Two Different Beef Cattle Breeds on Spring-Born Calves.

To investigate the impact of maternal microbiota during lactation in different beef cattle breeds on their own immune levels, milk quality, and the growth and development of their offspring, this study measured the immune parameters, intestinal microbiota diversity, and milk quality of Pingliang red cattle and Simmental cattle, and performed a correlation analysis with the growth and development of their offspring. Our study showed that during lactation, Pingliang red cattle had significantly higher IL-6 levels than Simmental cattle, while the latter exhibited higher levels of immune factors such as IgG, IgA, IgM, IL-1β, and TNFα. The analysis of the intestinal microbiota of lactating cows found that Pingliang red cattle were rich in and , while Simmental cattle had a higher proportion of Actinobacteria.

Quantitative Proteomics Analysis Reveals XDH Related with Ovarian Oxidative Stress Involved in Broodiness of Geese.

Studies have demonstrated significant alterations in ovarian oxidative stress levels, ovarian degeneration, and follicular atresia during the broody period in geese. The results of this study showed that during the broody period, geese exhibited degraded ovarian tissues, disrupted follicular development, a thinner granulosa cell layer, and lower levels of ovarian hormones E2, P4, and AMH. Antioxidant activity (GSH, CAT, SOD, T-AOC, and the content of HO) and the mRNA expression levels of antioxidant genes (GPX, SOD-1, SOD-2, CAT, COX-2, and Hsp70) were significantly higher in pre-broody geese compared to laying geese, while the expression of apoptosis-related genes (p53, Caspase-3, and Caspase-9) increased and the anti-apoptotic gene Bcl-2 decreased.

Formulation, Characterization, Antibacterial Activity, Antioxidant Activity, and Safety Evaluation of Essential Oil Nanoemulsions Through High-Pressure Homogenization.

The volatility, instability, and water insolubility of essential oil (CLEO) limit its practical applications in the food, pharmaceutical, and cosmetics industries. CLEO nanoemulsions (CLNEs) were formulated and characterized to overcome the aforementioned issues. The volatile compounds of CLEO were identified by gas chromatography-mass spectrometry.

Joint and independent associations of dietary antioxidant intakes with all-cause and cardiovascular mortality among patients with hypertension: a population-based cohort study.

Background: The evidence regarding dietary antioxidant intake and all-cause and cardiovascular disease (CVD) mortality among patients with hypertension is scarce.

Methods And Results: This study included 16,190 adults with hypertension from the National Health and Nutrition Examination Survey (NHANES) 1999-2018. Death outcomes were ascertained by linkage to National Death Index records through December 31, 2019.

The current status and influencing factors of diabetes knowledge among non-endocrinology nurses of tertiary general hospitals: a cross-sectional survey study.

Background: Every year, more than one-third of diabetes patients experience various acute and chronic complications, leading to the presence of diabetes patients in various departments of the hospital. High-quality nursing care can delay the progression of diabetes and effectively reduce the incidence of complications. Therefore, understanding the level of diabetes knowledge and training needs of clinical nurses is of great significance.

B cell translocation gene 2 expression levels in human granulosa cells is negatively associated with in vitro fertilization/intracytoplasmic sperm injection outcomes: a pilot study.

Purpose: This study aimed to monitor the expression of B-cell translocation gene 2 (BTG2) in granulosa cells of patients undergoing IVF/ICSI with respect blastocyst quality outcomes.

Methods: We recruited 181 women undergoing IVF/ICSI cycles for infertility. Granulosa cells were extracted from follicular fluid.

Comprehensive analysis of the transcriptome-wide m6A Methylome in sheep testicular development.

N6-methyladenosine (m6A) modification of RNA is a critical post-transcriptional modification, that dynamically contributes to testicular development and spermatogenesis. Nevertheless, the investigation into the role of m6A in testicular development of sheep remains insufficient. Herein, we conducted a comprehensive analysis of the m6A transcriptome landscape in the testes of F1 hybrid Southdown × Hu sheep across M0 (0 months old, newborn), M3 (3 months old, sexually immature), M6 (6 months old, sexually mature), and Y1 (1 years old, adult).

Cardiometabolic benefits of a non-industrialized-type diet are linked to gut microbiome modulation.

Industrialization adversely affects the gut microbiome and predisposes individuals to chronic non-communicable diseases. We tested a microbiome restoration strategy comprising a diet that recapitulated key characteristics of non-industrialized dietary patterns (restore diet) and a bacterium rarely found in industrialized microbiomes (Limosilactobacillus reuteri) in a randomized controlled feeding trial in healthy Canadian adults. The restore diet, despite reducing gut microbiome diversity, enhanced the persistence of L.

When low-light meets flares: Towards Synchronous Flare Removal and Brightness Enhancement.

Low-light image enhancement (LLIE) aims to improve the visibility and illumination of low-light images. However, real-world low-light images are usually accompanied with flares caused by light sources, which make it difficult to discern the content of dark images. In this case, current LLIE and nighttime flare removal methods face challenges in handling these flared low-light images effectively: (1) Flares in dark images will disturb the content of images and cause uneven lighting, potentially resulting in overexposure or chromatic aberration; (2) the slight noise in low-light images may be amplified during the process of enhancement, leading to speckle noise and blur in the enhanced images; (3) the nighttime flare removal methods usually ignore the detailed information in dark regions, which may cause inaccurate representation.

Factors associated with higher caregiver burden among informal caregivers of Parkinson's disease: A systematic review.

Background: Parkinson's disease is a progressive neurodegenerative disease and the care burden in informal caregivers is huge. Summarizing factors associated with the informal caregivers burden can improve our understanding of providing proactive support to informal caregivers caring for patients with Parkinson's disease (PwP) at risk, and provides evidence for clinical practice.

Methods: PRISMA guidelines were followed in this systematic review.

Variants in the SOX9 transactivation middle domain induce axial skeleton dysplasia and scoliosis.

SOX9 is a crucial transcriptional regulator of cartilage development and homeostasis. Dysregulation of is associated with a wide spectrum of skeletal disorders, including campomelic dysplasia, acampomelic campomelic dysplasia, and scoliosis. Yet how variants contribute to the spectrum of axial skeletal disorders is not well understood.

Metalgel Fiber with Excellent Electrical and Mechanical Properties.

With the rapid advancement of soft electronics, particularly the rise of fiber electronics and smart textiles, there is an urgent need to develop high-performance fiber materials with both excellent electrical and mechanical properties. However, existing fiber materials including metal fibers, carbon-based fibers, intrinsically conductive polymer fibers, and composite fibers struggle to simultaneously meet the requirements. Here, we introduce a metalgel fiber with a unique structure.

Identification of PANoptosis associated lncRNAs associated with clinical prognosis and immune infiltration microenvironment in colon adenocarcinoma.

Early diagnosis and disease management based on risk stratification have a very positive impact on colon adenocarcinoma (COAD) prognosis. It is of positive significance to further explore risk stratification of COAD patients and identify predictive molecular biomarkers. PANoptosis is defined as a form of inflammatory cell death regulated by PANoptosome, with common features of pyroptosis, apoptosis and necroptosis.

CFAP65 is essential for C2a projection integrity in axonemes: implications for organ-specific ciliary dysfunction and infertility.

Defects in motile cilia and flagella lead to motile ciliopathies, including primary ciliary dyskinesia (PCD), which manifests as multi-organ dysfunction such as hydrocephalus, infertility, and respiratory issues. CFAP65 variants are a common cause of male infertility, but its localization and function have remained unclear. In this study, we systematically evaluated CFAP65's role using Cfap65 knockout mice and human patients with CFAP65 variants.

Identify potential drug candidates within a high-quality compound search space.

The identification of potential effective drug candidates is a fundamental step in new drug discovery, with profound implications for pharmaceutical research and the healthcare sector. While many computational methods have been developed for such predictions and have yielded promising results, two challenges persist: (i) The cold start problem of new drugs, which increases the difficulty of prediction due to lack of historical data or prior knowledge. (ii) The vastness of the compound search space for potential drug candidates.

The Development of a Novel Broad-Spectrum Influenza Polypeptide Vaccine Based on Multi-Epitope Tandem Sequences.

Background: Polypeptide vaccines have the potential to improve immune responses by targeting conserved and weakly immunogenic regions in antigens. This study aimed to identify and evaluate the efficacy of a novel influenza universal vaccine candidate consisting of multiple polypeptides derived from highly conserved regions of influenza virus proteins hemagglutinin (HA), neuraminidase (NA), and matrix protein 2 (M2).

Methods: Immunoinformatics tools were used to screen conserved epitopes from different influenza virus subtypes (H1N1, H3N2, H5N1, H7N9, H9N2, and IBV).

Integrated Transcriptomic and Targeted Metabolomic Analysis Reveals the Key Genes Involved in Triterpenoid Biosynthesis of .

is a traditional Chinese medicinal fungus, and ganoderma triterpenoids (GTs) are one of the main bioactive compounds. These compounds have various pharmacological functions, including anti-tumor, antioxidant, anti-inflammatory, liver-protective, and immune-regulating effects. However, the manner in which they accumulate, and their biosynthesis mechanisms remain unclear.

The Electric Field Guided HaCaT Cell Migration Through the EGFR/p38 MAPK/Akt Pathway.

Previous studies have shown that the endogenous electric field (EF) is an overriding cure in guiding cell migration toward the wound center to promote wound healing, but the mechanism underlying is unclear. In this study, we investigated the molecular mechanism of electric field-guided cell migration in human keratinocyte HaCaT cells. Our results showed that HaCaT cells migrate toward the anode under EFs.

Image-Based Monitoring of Thermal Ablation.

Thermal therapy is a commonly used local treatment technique in clinical practice. Monitoring the treatment process is essential for ensuring its success. In this review, we analyze recent image-based methods for thermal therapy monitoring, focusing particularly on their feasibility for synchronous or immediate postoperative monitoring.

A chromosome-scale genome assembly of the pioneer plant Stylosanthes angustifolia: insights into genome evolution and drought adaptation.

Background: Drought is a major limiting factor for plant survival and crop productivity. Stylosanthes angustifolia, a pioneer plant, exhibits remarkable drought tolerance, yet the molecular mechanisms driving its drought resistance remain largely unexplored.

Results: We present a chromosome-scale reference genome of S.

Numerical Simulation of Fluid Shear Stress Distribution in Microcracks of Trabecular Bone.

Bone is one of the hardest tissues in the human body, but it can undergo microcracks under long-term and periodic mechanical loads. The Newton iterative method was used to calculate the steady state, and the effects of different inlet and outlet pressures, trabecular gap width and height, and microcrack's depth and width on the fluid shear stress (FSS) were studied, and the gradient of FSS inside the microcrack was analyzed. The results show that the pressure difference and trabecular gap heigh are positively correlated with the FSS (the linear correlation coefficients were 0.

Characterization and pathogenicity of a novel avian orthoreovirus in China.

Introduction: Avian orthoreovirus (ARV) is a significant pathogen causing viral arthritis, leading to substantial economic losses in the poultry industry worldwide.

Methods: A novel ARV strain, designated FJ202311, was isolated from a broiler farm in Fujian Province, China. Whole-genome sequencing was conducted using next-generation sequencing with MGI technology, and phylogenetic analysis of the sigma C amino acid sequence was performed.