A near-infrared "turn-on" fluorescent probe for selective detection of copper(II) ions in aqueous media and its application in cell imaging.

Copper (II) is considered the third most essential trace element for life, following iron and zinc, and plays a vital role in various physiological and pathological processes. The fluorescent probe has become an important method for the detection of heavy metal ions. This investigation involved the development and synthesis of a near-infrared "turn-on" fluorescent probe, DCX-Cu, with a significant Stokes shift (156 nm), specifically to identify Cu compared other ions.

Maternal high-fat diet disrupts intestinal mucus barrier of offspring by regulating gut immune receptor LRRC19.

Maternal high fat diet (MHFD) increased colitis susceptibility in adulthood. However, the mechanism remains unclear. We sought to explore whether novel gut immune receptor leucine-rich repeat C19 (LRRC19) contributed to the impaired mucus barrier of offspring exposed to MHFD via gut immune response and microbiota.

Quad-Nanopore Array Enables High-Resolution Identification of Four Single-Stranded DNA Homopolymers.

The solid-state nanopore technique holds the potential to develop mechanically stable and miniaturized DNA sequencing devices. However, the limited temporal resolution due to the high electric field inside the nanopore and the lack of an effective speed control strategy have hindered the realization of sequencing. Here, we reported a quad-array (four nanopores milled with ∼30 nm interpore spacing as a detection unit) that induced a redistribution of the electric field inside and outside the nanopore array and offered high-resolution discrimination of four ssDNA homopolymer types.

Self-powered droplet manipulation for full human-droplet interaction in multiple mediums.

Droplet manipulation holds significant promise across the energy, environmental, and medical fields. However, current methods still lack a solution that simultaneously satisfies the requirements for self-powered energy supply, high efficiency, human-droplet interaction, flexibility, and universality. Herein, we develop a human-droplet interaction platform based on an omni-directional triboelectric tweezer, which directly utilizes triboelectric charges induced by human motion to manipulate droplets.

Choroidal changes and association with Gaussian curvature in high myopic eyes with posterior staphyloma in ultra-wide-field optical coherence tomography angiography.

Purpose: To evaluate choroidal changes in high myopic (HM) patients with posterior staphyloma (PS) and explore their association with Gaussian curvature (K) using ultra-wide-field optical coherence tomography angiography (UWF-OCTA).

Methods: HM patients with wide macular PS (Type I PS) and without any PS (control) were recruited. Highest K values (K) and choroidal parameters-vessel flow density (VFD), choroidal vascularity volume (CVV) and index (CVI), thickness, and volume-were compared in central and peripheral regions, and the fovea-centered 12-mm ETDRS quadrants of 24 × 20 mm UWF-OCTA images.

Utilization and Influencing Factors of Mobile Health Applications in Diabetes Management: An Integrative Review.

Mobile health applications are increasingly acknowledged as effective instruments for enhancing self-management among individuals with diabetes. However, despite their potential, the extent of their global usage among this population remains unclear. To better understand this, we conducted a systematic review of existing studies to explore the worldwide usage of mobile health applications by this population and to identify factors that influence their usage.

Polystyrene microplastics impaired the function of leydig cells via GRP78/PERK/CHOP mediated endoplasmic reticulum stress in vivo and in vitro.

The toxic effect of Polystyrene Microplastics (PS-MPs) on leydig cells were found in male mice, but the toxic mechanism was not clear. The PS-MPs exposure mice model and cell model were established in this study to explore the leydig cells toxic mechanism. In vivo study, the leydig cells toxicity in male mice was evaluated exposed to PS-MPs for 28 days.

Application of large language models in healthcare: A bibliometric analysis.

Objective: The objective is to provide an overview of the application of large language models (LLMs) in healthcare by employing a bibliometric analysis methodology.

Method: We performed a comprehensive search for peer-reviewed English-language articles using PubMed and Web of Science. The selected articles were subsequently clustered and analyzed textually, with a focus on lexical co-occurrences, country-level and inter-author collaborations, and other relevant factors.

Preclinical study of inetetamab combined with atezolizumab to synergistically inhibit HER2 and PD-L1 in the treatment of ovarian cancer.

Epithelial ovarian cancer (EOC) is the deadliest gynecological malignancy. Precision treatments are crucial for improving patient survival. This research explored the potential anti-tumor effects of combining inetetamab and atezolizumab for HER2 EOC patients.

Role of triggering receptor expressed on myeloid cells 2 in the pathogenesis of non-alcoholic fatty liver disease.

Non-alcoholic fatty liver disease (NAFLD) is a progressive disease. Without effective interventions, NAFLD can gradually develop to non-alcoholic steatohepatitis, fatty liver fibrosis, liver cirrhosis and even hepatocellular carcinoma. It is still to investigate the precise molecular mechanism behind the pathophysiology of NAFLD.

DDX5 Alleviates Temporomandibular Joint Osteoarthritis via TNF-Induced NF-κB Signaling Pathway.

Background: Temporomandibular joint osteoarthritis (TMJOA) is a prevalent musculoskeletal condition characterized by pain, cartilage degeneration, and subchondral bone loss.

Objective: This study sought to identify specific targets for the treatment of TMJOA.

Method: Through high-throughput RNA-seq analysis in condylar chondrocytes (NC vs.

Mitochondrial RNA metabolism, a potential therapeutic target for mitochondria-related diseases.

In recent years, the roles of mitochondrial RNA and its associated human diseases have been reported to increase significantly. Treatments based on mtRNA metabolic processes and nuclear gene mutations are thus discussed. The mitochondrial oxidative phosphorylation process is affected by mtRNA metabolism, including mtRNA production, maturation, stabilization, and degradation, which leads to a variety of inherited human mitochondrial diseases.

Klippel-Trenaunay syndrome with multiorgan vascular involvement and gastrointestinal bleeding: A case report and literature review.

Rationale: To explore the early diagnosis and treatment of Klippel-Trenaunay syndrome (KTS), and provide useful and systematic clinical references for the diagnosis and treatment of such congenital vascular malformations combined with long-term gastrointestinal bleeding.

Patient Concerns: A 32-year-old male patient was admitted due to "intermittent rectal bleeding for 32 years, worsening for over 3 months." The patient had experienced intermittent bright red rectal bleeding since birth.

The association between a history of induced abortion for nonmedical reasons and maternal and neonatal perinatal outcomes: a retrospective cohort study.

Introduction: Following the implementation of the universal two-child policy in China, many multiparous women who had a history of induced abortion gave birth again. However, there is a lack of studies exploring the associations between induced abortion for nonmedical reasons and maternal and neonatal perinatal complications.

Methods: In this retrospective cohort study, the participants were multiparous women who gave birth to singleton babies at or after 28 weeks of gestation between 1 December 2015, and 1 December 2020.

Hydroxypropyl trimethyl ammonium chloride chitosan (HACC)-modified protein nanoparticles enhance docetaxel oral delivery and exhibit potent in vitro anti-tumor and macrophage-activating effects.

The antitumor drug docetaxel (DTX) has limited oral bioavailability. This study aimed to develop novel oral nanoparticles to enhance DTX's absorption and bioactivities. The nanoparticles (H-N-D) were prepared by inducing the self-assembly of a Coptis protein through heating, followed by modification using hydroxypropyl trimethyl ammonium chloride chitosan (HACC).

S1PR1-biased activation drives the resolution of endothelial dysfunction-associated inflammatory diseases by maintaining endothelial integrity.

G protein-coupled sphingosine-1-phosphate receptor 1 (S1PR1), a drug target for inflammatory bowel disease (IBD), enables immune cells to egress from lymph nodes, but the treatment increases the risk of immunosuppression. The functional signaling pathway triggered by S1PR1 activation in endothelial cells and its therapeutic application remains unclear. Here, we showed that S1PR1 is highly expressed in endothelial cells of IBD patients and positively correlated with endothelial markers.

Exploring the shared genetic mechanisms of atopic dermatitis and alopecia areata via bioinformatics approaches.

Atopic dermatitis (AD) and alopecia areata (AA) are chronic inflammatory skin diseases. While studies suggest a possible immune defense mechanism link between the two, the causal relationship remains unclear, and current methodologies have limitations. This study aims to investigate the causal relationship between AD and AA using genome-wide association study statistics from the Integrative Epidemiology Unit Open Genome-Wide Association Study (IEU Open Gwas) project.

Association of insulin resistance surrogates with disease severity and adverse outcomes in chronic thromboembolic pulmonary hypertension: a multicenter cohort study.

Background: Chronic thromboembolic pulmonary hypertension (CTEPH) is a severely progressive disease that leads to right heart failure and death. Previous studies have shown that diabetes and insulin resistance (IR) are closely related to pulmonary hypertension, but the role of IR in patients with CTEPH remains unexplored. In this study, we investigated the relationship between four insulin resistance indices and disease severity, hemodynamic parameters, and adverse outcomes in patients with CTEPH.

Design of CoQ crops based on evolutionary history.

Coenzyme Q (CoQ) is essential for energy production by mitochondrial respiration, and it is a supplement most often used to promote cardiovascular health. Humans make CoQ, but cereals and some vegetable/fruit crops synthesize CoQ with a side chain of nine isoprene units. Engineering CoQ production in crops would benefit human health, but this is hindered by the fact that the specific residues of the enzyme Coq1 that control chain length are unknown.

The effect of Yinhuang Hanhua dropping pill on acute pharyngitis via a systematic "Compound-Target-Pathway" analysis.

Background: Yinhuang Hanhua dropping pill (YHHHDP) is a Chinese herbal formula that is commonly used in medical practice for managing of acute pharyngitis (AP) and other upper respiratory tract infections. However, the active components, molecular targets, and mechanisms underlying its therapeutic effects remain poorly understood.

Purpose: The objective of this study was to identify the active components and molecular mechanisms of YHHHDP in the therapy of AP.

Exploring Construction of Biomedical Ti6Al4V-Ti5Cu Composite Alloy with Interpenetrating Structure: Microstructure and Corrosion Resistance.

Cu-bearing titanium alloys exhibit promising antibacterial properties for clinical use. A novel Ti6Al4V-Ti5Cu composite alloy is developed using powder bed fusion (selective laser sintering, SLM) and spark plasma sintering (SPS). SLM produces a triple periodic minimal surface (TPMS) lattice structure from Ti6Al4V, which is then filled with Ti-5Cu powders and sintered using SPS.

miR-758-3p Interferes with Neuronal Apoptosis in Cerebral Ischemia-Reperfusion by Inhibiting ILK.

This study investigated the role of integrin-linked kinase (ILK) in neuronal apoptosis induced by cerebral ischemia‒reperfusion injury (CIRI) and its interaction with a circRNA (0000964) and miR-758-3p. Using in vivo and in vitro rat models, we clarified how ILK regulates neuronal apoptosis during CIRI. Our findings revealed that ILK expression is upregulated in response to CIRI and is modulated by the circRNA (0000964)/miR-758-3p axis.

Revealing Mechanisms of Lithium-Mediated Nitrogen Reduction Reaction from First-Principles Simulations.

Recently, lithium-mediated nitrogen reduction reaction (Li-NRR) in nonaqueous electrolytes has proven to be an environmentally friendly and feasible route for ammonia electrosynthesis, revealing tremendous economic and social advantages over the industrial Haber-Bosch process which consumes enormous fossil fuels and generates massive carbon dioxide emissions, and direct electrocatalytic nitrogen reduction reaction (NRR) which suffers from sluggish kinetics and poor faradaic efficiencies. However, reaction mechanisms of Li-NRR and the role of solid electrolyte interface (SEI) layer in activating N remain unclear, impeding its further development. Here, using electronic structure theory, we discover a nitridation-coupled reduction mechanism and a nitrogen cycling reduction mechanism on lithium and lithium nitride surfaces, respectively, which are major components of SEI in experimental characterization.

From bench to bedside: Developing CRISPR/Cas-based therapy for ocular diseases.

Vision-threatening disorders, including both hereditary and multifactorial ocular diseases, necessitate innovative therapeutic approaches. The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated protein (Cas) has emerged as a promising tool for treating ocular diseases through gene editing and expression regulation. This system has contributed to the development of representative disease models, including animal models, organoids, and cell lines, thereby facilitating investigations into the pathogenesis of disease-related genes.

Effectiveness of using implementation frameworks to facilitate the implementation of a stroke management guideline in the traditional Chinese medicine hospitals in China: protocol for a factorial randomised controlled trial.

Introduction: Traditional Chinese medicine (TCM) is commonly used alongside Western medicine for stroke management in China. However, there is significant variation in TCM practice, and the utilisation of evidence-based clinical practice guidelines is inadequate. This study aims to evaluate the effectiveness of three popular frameworks-Consolidated Framework for Implementation Research (CFIR), Theoretical Domains Framework (TDF) and Normalization Process Theory (NPT)-in improving implementation outcomes for the integrated TCM and Western medicine clinical practice guideline for stroke management.