Bmpallidin, encoding a subunit of the BLOC-1 complex, is involved in urate granules formation in silkworm integument.

The abnormal development of urate granules in silkworm larvae leads to translucent mutants with a distinct transparent phenotype. Studies on such mutants are expected to enhance current understanding of uric acid metabolism. The hoarfrost translucent (oh) mutant exhibits a mottled, translucent larval integument due to the presence of smaller and irregularly shaped urate granules compared to wild-type individuals.

Hydrogeochemical mechanism of Li-Cs-rich thermal springs in the Karakoram Fault, Qinghai-Xizang Plateau: New insights from multiple stable isotopes.

High lithium (Li) and cesium (Cs) concentrations in the Qinghai-Xizang Plateau thermal springs pose environmental and health challenges, but their origins and enrichment mechanisms remain unclear. This study focuses on the Sogdoi geothermal field, located along the southern Karakoram Fault, to investigate these processes. Multi-isotope analyses (H, O, Li, Sr) reveal that Li and Cs predominantly originate from the host rocks, especially granitoids and meta-sedimentary rocks, rather than from magmatic fluids.

Long-Term Cardiovascular Complications in Patients With Pheochromocytomas and Paragangliomas After Surgery: A Large Multi-Center Study.

Objective: The effects of pheochromocytomas and paragangliomas (PPGLs)-induced catecholamine overproduction on vascular and cardiac function are generally thought to be reversible after PPGLs removal. However, a sizable proportion of patients who were free of the recurrent disease still faced high risks of cardiovascular problems after successful surgery. We aim to identify incidence and risk factors for long-term cardiovascular complications in PPGLs patients after surgery.

Caragana jubata ethanol extract ameliorates the symptoms of STZ-HFD-induced T2DM mice by PKC/GLUT4 pathway.

Ethnopharmacological Relevance: Caragana jubata (Pall.) Poir., a traditional Tibetan medicinal plant in China, is renowned in Tibetan medicine for its hypoglycemic properties and long-standing use in treating diabetes.

Trim72 is a major host factor protecting against lethal Candida albicans infection.

Candida albicans is the most common aetiologic pathogen of fungal infections associated with high mortality in immunocompromised patients. There is an urgent need to develop new antifungal therapies owing to the poor efficacy and resistance of current antifungals. Here, we report that Trim72 positively regulates antifungal immunity during lethal fungal infection.

Preparation of soybean isolate protein/xanthan gum/agar-Lycium ruthenicum anthocyanins intelligent indicator films and its application in mutton preservation.

The preparation of intelligent indicator films containing anthocyanins and their utilization for real-time monitoring of meat freshness represents a prominent research topic of food packaging. In this study, anthocyanins (ALR) were extracted from Lycium ruthenicum (LR) using solvent extraction. Subsequently, these anthocyanins were incorporated into films composed of soybean isolate protein (SPI), xanthan gum (XG) and agar, resulting in SPI/XG/Agar-ALR pH-responsive intelligent indicator films.

Revealing the dynamic changes of metabolites and molecular mechanisms of chlorogenic acid accumulation during the leaf development of based on multi-omic analyses.

, a medicinal plant, is utilized for Quezui Tea production from its leaf buds and young leaves. Despite prior research on revealing several medicinal compounds, the comprehensive variations in metabolites during its growth and development, along with the molecular mechanisms underlying high chlorogenic acid (CGA) yield, remain unclear. Through a joint analysis of transcriptomics and proteomics, our study first identified 15 key structural genes and 3 transcription factors influencing CGA biosynthesis in , offering new evidence to understand its regulatory network.

Patient-reported gastrointestinal symptoms in gastric cancer after laparoscopic distal gastrectomy.

Purpose: This study aimed to compare postoperative gastrointestinal symptoms between patients who underwent laparoscopic distal gastrectomy with Roux-en-Y (R-Y) and Billroth-II with Braun (B-II B) reconstruction.

Methods: This observational study retrospectively analyzed 151 patients (110 in R-Y group and 41 in B-II B group) who underwent laparoscopic distal gastrectomy from January 2020 to December 2021. A comparison was made regarding surgical outcomes, perioperative nutritional and inflammatory markers, postoperative dietary patterns, and gastrointestinal symptoms between the two groups.

Sevoflurane postconditioning mitigates neuronal hypoxic-ischemic injury via regulating reactive astrocytic STAT3 protein modification.

Astrocyte activation plays a pivotal role in accelerating the cascade of neuroinflammation associated with the development of hypoxic-ischemic brain injury. This study aimed to investigate the mechanism by which sevoflurane postconditioning mitigates neuronal damage through astrocytes by regulating reactive astrocytic Signal Transducer and Activator of Transcription 3 (STAT3) modifications. A modified Rice‒Vannucci model in rats and a conditioned culture system established by subjecting primary astrocytes to oxygen glucose deprivation, followed by using the conditioned medium to culture the neuron cell line SH-SY5Y were used to simulate HI insult in vivo and in vitro, respectively.

Physical activity affects dysthyreosis by thyroid hormones sensitivity: a population-based study.

Objective: Physical activity (PA) plays an important role in human health. However, the relationship between the PA and dysthyreosis was not clear. This study aimed to explore this question.

Research Progress of Shared Decision-Making in Schizophrenia with Anxiety.

Shared decision-making (SDM) has been practiced fairly frequently in general medicine, but less so in mental disorders, especially in closed psychiatric wards, and its positive predictor has recently been recognized in the field of mental health. Studies have shown that patients with severe mental disorders want a say in their care and their desire to participate in clinical decision-making is even higher than those treated by general medical services. This review aims to explore the research progress of SDM for patients with schizophrenia with anxiety in a collectivist culture (such as Chinese culture) and to provide a reference for the implementation of SDM in closed psychiatric wards.

Engineered bispecific antibodies with enhanced breadth and potency against SARS-CoV-2 variants and SARS-related coronaviruses.

The concern of COVID-19 persists due to the continuous emergence of variants and the potential spillover of animal coronaviruses. The broad-spectrum neutralizing antibodies play a pivotal role in the prevention and treatment of coronavirus (CoV) infections. Here, we constructed 18 bi-specific antibodies (bsAbs) using 9 antibodies isolated from COVID-19 convalescents and vaccinated individuals, designed as dual variable domain immunoglobulin (DVD-Ig).

Two Novel Fluorescence Probes Based on Caffeic Acid Derivative for Phosphate Ions and Their Applications in Biological Samples.

Phosphate is widely used in industry and agriculture fields. However, excess accumulation of PO causes several adverse effects on the human body and ecological environment. Consequently, it is important to develop a simple method for the detection of PO concentration in the ecological environment and in vivo.

Bioactivity-Guided Isolation of Secondary Metabolites from : Antioxidative Antibacterial Activities and Anti-Inflammatory Hypoglycemic Molecular Docking.

is a valuable ornamental, edible, and medicinal plant with promising prospects for bioactivity development. We screened the bioactivity of eight fractions (Fr. A-I) obtained from the ethyl acetate phase of via silica gel column chromatography.

Downregulation of transcription factor promoted nucleopolyhedrovirus replication in .

The Janus kinase/signal transducer and activator of transcription (JAK/STAT) signaling pathway plays a crucial role in the viral immune processes of organisms, with STAT being a key transcription factor downstream in this pathway. The gene of has two splicing forms, named and . This study compared the effects of the two splicing forms on nucleopolyhedrovirus (BmNPV) infection through cell-level interference and further explored whether participates in the immune response to BmNPV infection via transgenic intervention at the individual level.

Dexmedetomidine Improves Long-term Neurological Outcomes by Promoting Oligodendrocyte Genesis and Myelination in Neonatal Rats Following Hypoxic-ischemic Brain Injury.

Neonatal hypoxic-ischemic brain injury (HIBI) can lead to white matter damage, which significantly contributes to cognitive dysfunction, emotional disorders, and sensorimotor impairments. Although dexmedetomidine enhances neurobehavioral outcomes, its impact on oligodendrocyte genesis and myelination following hypoxic-ischemic events, as well as the underlying mechanisms, remain poorly understood. Dexmedetomidine was administered 15 min post-HIBI.

GC-MS-based metabonomic analysis of silkworm haemolymph reveals four-stage metabolic responses to nucleopolyhedrovirus infection.

Silkworm, Bombyx mori, an economically significant insect, plays a crucial role in silk production. However, silkworm breeding is highly susceptible to various pathogens, particularly the Bombyx mori nucleopolyhedrovirus (BmNPV), which poses a serious threat. Recent metabonomic studies have provided insights into the metabolic changes associated with BmNPV infection.

A mutation in the Bombyx mori BmSPI51 gene results in alterations protein abundance in cocoon and unveils compensatory mechanism of silk gland proteinase inhibitors.

Proteinase inhibitors constitute a significant component of Lepidoptera cocoon. BmSPI51 is the most abundant protease inhibitors in silkworm cocoons. In this study, we aimed to elucidate the biological function of BmSPI51 in both the silkworm silk gland and cocoon formation.

[NF-κB transcription factors regulate the expression of immunity-related genes in different cell lines of silkworm ()].

The Toll and immune deficiency (IMD) signaling pathways in insects are highly conserved in evolution and regulate the expression of antimicrobial peptides (AMPs) and other immune-related genes mainly through nuclear factor-kappa B (NF-κB) transcription factors. However, the differences of NF-κB transcription factors Rels and Relish in the expression regulation of AMPs and other immune-related genes in silkworm () have not been systematically reported. In this study, the , and genes were cloned and their eukaryotic cell overexpression vectors were constructed.

Taste characterization and molecular docking study of novel umami flavor peptides in Yanjin black bone Chicken meat.

Five polypeptides with a potential umami taste were isolated and purified from Yanjin black bone chicken. However, the flavor characteristics and umami mechanism have not been clarified. The umami properties of these five peptides were investigated in this work using a range of analytical techniques, computer simulation, and sensory evaluation.

Seven undescribed compounds from dried flower buds of Ochrocarpus longifolius.

Seven unreported compounds were isolated from dried flower buds of Ochrocarpus longifolius in this study, including an alkaloid ochrocaracid A (1), five coumarins ochrocarpins I (2), J (3), K (4), L (5), and M (6), and one styryl-2-pyranone compound iresinoacid (7) and nine known compounds (8-16). All these compounds were found in O. longifolius for the first time.

PCSK9 inhibitor protects against myocardial ischemia-reperfusion injury via inhibiting LRP8/GPX4-mediated ferroptosis.

Myocardial ischemia-reperfusion injury is accompanied by ferroptosis mediated by reactive oxygen species and iron ions, which aggravates myocardial tissue damage. The present study aims to explore the molecular mechanism underlying the mitigating effects f PCSK9 on myocardial ischemia-reperfusion injury. MI/R rat model and OGD/R induced H9c2 model were established.

In vivo and in vitro Reconstitution of Biosynthesis of N-Prenylated Phenazines Revealing Diverse Phenazine-Modifying Enzymes.

Phenazine natural products play various roles such as signal molecules, antibiotics, or electron carriers in their producer strains. Among these products, phenazinomycin and lavanducyanin, which are produced by Streptomyces species, are characterized by an N-alkyl modification. Herein, we established the biosynthetic pathways for these two phenazine natural products.

Transcriptomic Analysis of Genes Associated with Stinger Development at Different Life Stages of .

Stingers, evolved from ovipositors, are an important defense organ for the , , and species. However, the molecular mechanism of stinger development remains unclear. Here, we show that the earliest time point for the appearance of stingers in is at the 1-day-old worker pupal stage based on morphological observations and anatomy from the pre-pupal to adult stages.