Crossbreeding Simmental with Mongolian, and Holstein cattle can improve feed efficiency and energy metabolism by upregulating COX3 and downregulating PRSS2 gene expression.

The selective breeding of beef cattle plays an important role in meeting the growing demand for beef and improving production performance. This study used fattened cattle of the Simmental (S) breed, and two crossbreeds: Simmental × Mongolian (SM) and Simmental × Holstein (SH), which were healthy, of similar age and weight. The results showed that the blood glucose (GLU) levels of the crossbred, genetically improved SM and SH groups were higher than that of the S group.

Facile minocycline deployment in gingiva using a dissolvable microneedle patch for the adjunctive treatment of periodontal disease.

Minocycline is a commonly used drug for adjunctive therapy in periodontal disease. However, the current mainstream local medications primarily rely on intra-pocket administration, which, while avoiding the side effects of traditional systemic drugs, presents challenges such as inconvenience, discomfort, and the need for professional assistance, thus affecting patient compliance. Herein, we introduce a minocycline-loaded dissolvable microneedle (Mino-DMN) patch that allows for local and efficient delivery of minocycline to gingiva for the treatment of periodontitis.

DX-9 biocontrol against potato common scab involves significant changes in the soil microbiome and metabolome.

Unlabelled: Potato common scab (CS) is a worldwide disease, caused by spp., and its presence reduces the market value of potatoes. A nontoxic and potentially effective approach in many control strategies is the use of antagonistic microbes as biocontrol agents.

NONO interacts with nuclear PKM2 and directs histone H3 phosphorylation to promote triple-negative breast cancer metastasis.

Background: Emerging evidence has revealed that PKM2 has oncogenic functions independent of its canonical pyruvate kinase activity, serving as a protein kinase that regulates gene expression. However, the mechanism by which PKM2, as a histone kinase, regulates the transcription of genes involved in triple-negative breast cancer (TNBC) metastasis remains poorly understood.

Methods: We integrated cellular analysis, including cell viability, proliferation, colony formation, and migration assays; biochemical assays, including protein interaction studies and ChIP; clinical sample analysis; RNA-Seq and CUT&Tag data; and xenograft or mammary-specific gene knockout mouse models, to investigate the epigenetic modulation of TNBC metastasis via NONO-dependent interactions with nuclear PKM2.

Establishing a database of volatile metabolites in whole wheat (Triticum aestivum L.) flour provides novel insights into quality breeding.

Improving yield traits is the primary goal for wheat breeding, while much less attention has been paid to flavor and odor quality of wheat grain. In this study, a high-throughput protocol was developed for the identification and quantification of volatile compounds (VOCs) in wheat grains via a static head-space coupled with GC-MS. Using a diverse population consisting of 312 wheat germplasms, a database including 94 VOCs was constructed for grains.

Isolation and characterization of a novel oxyphenisatin analogue, 4-Chloro-oxyphenisatin diisobutyrate, from a jelly candy purported to possess weight-loss properties.

A novel oxyphenisatin analogue was identified in a type of jelly candy during routine inspections of food products marketed for weight-loss purposes. Through analysis utilizing ultra-high-performance quadrupole-Orbitrap high-resolution mass spectrometry (UHPLC-Q-Orbitrap-HRMS), the fragment ions at m/z 258 and 195 observed in the MS/MS experiments were found to be consistent with those of 4-Chloro-oxyphenisatin diacetate. It was inferred that the unknown compound is likely a derivative of 4-Chloro-oxyphenisatin diacetate.

Photocatalytic activation of peroxymonocarbonate with a TiO catalyst for water remediation.

The novel advanced oxidation process was constructed to remove pollutants by activating in-situ generated peroxymonocarbonate (HCO) through photocatalysis, using commercially available rutile TiO as the photocatalyst. It demonstrated exceptional oxidative capacity, achieving complete degradation of acid red (AR73) within 30 min, and efficiently oxidizing various recalcitrant organic pollutants. Further, the photocatalytic system had a good resistance to interference and was smoothly conducted in a variety of water matrix with a wide range of pH (3-9).

Improving light use efficiency models via the introduction of both the diffuse fraction and radiation scalar.

Surface solar radiation, both its components and intensity, is pivotal in determining vegetation light use efficiency (LUE) and is essential for accurately estimating gross primary production (GPP) in ecosystems. This study introduces two key parameters: the diffuse photosynthetic photon flux density fraction (f) to account for the diffuse fertilization effect (DFE) and the radiation scalar to reflect the impact of radiation intensity on leaf-level LUE. Leveraging these parameters, we developed two novel LUE models: the Big-leaf Diffuse-fraction Radiation-scalar LUE (BDR-LUE) model, adapted from traditional big-leaf LUE models, and the Two-leaf Diffuse-fraction Radiation-scalar LUE (TDR-LUE) model, based on conventional two-leaf LUE frameworks.

Serial multiple mediation of perceived stress and self-control in the relationship between neuroticism and career anxiety among nursing interns in China: a cross-sectional questionnaire survey.

Background: Nursing internship play a crucial role in cultivating nursing students. However, nursing interns often face challenges of career anxiety, which can have negative impacts on their professional development and work quality. Therefore, understanding the factors influencing career anxiety among nursing interns is important.

Long non-coding RNA LINC01232 promotes malignancy of prostate cancer through regulation of miR-181a-5p/IRS2 pathway: Ki-67 protein molecular structure and function.

The expression level of long non-coding RNA (lncRNA), which functions in a manner similar to that of microrNA, has been confirmed to be closely associated with the regulatory network of multiple cancers. The primary focus of this particular research endeavor was to elucidate the mechanism of action of LINC01232 within the context of prostate cancer, specifically examining how it influences the proliferation of prostate cancer cells by interacting with the miR-181a-5p/IRS2 pathway. Additionally, the study aimed to delve deeper into the role of the Ki-67 protein within this intricate process.

Stapes footplate's posterior border protrudes the vestibule in healthy ears: anatomical insights from ultra-high-resolution CT.

Background: The stapes footplate (SF) and annular ligament (AL) in the oval window region are of paramount significances. This study aims to assess the visibility of AL and the relative positioning of the SF and vestibule using ultra-high-resolution computed tomography (U-HRCT).

Methods: U-HRCT images between September 2020 and April 2023 were retrospectively reviewed, and 479 ears deemed healthy from both clinical and radiological perspectives were included.

Updates on medical and surgical managements of diabetic retinopathy and maculopathy.

Diabetic retinopathy (DR) and diabetic macular edema (DME) are leading causes of vision loss globally. This is a comprehensive review focused on both medical and surgical management strategies for DR and DME. This review highlights the epidemiology of DR and DME, with a particular emphasis on the Asia-Pacific region, urban-rural disparities, ethnic variations, and grading methodologies.

Capsid protein of turnip crinkle virus suppresses antiviral RNA decay by degrading Arabidopsis Dcp1 via ubiquitination pathway.

RNA decay is a pervasive process in eukaryotic cells. Viruses utilize the host cell's intracellular machinery to gain access to essential molecules and subcellular structures required for infection during the pathogenesis process. The study demonstrates that turnip crinkle virus (TCV) infection enhances the expression of Arabidopsis Dcp1 (AtDcp1), which negatively regulates the accumulation of TCV RNA, indicating its involvement in antiviral defense.

Ultrasound speckle tracking imaging measurement of endocardial longitudinal strain for evaluation of prognostic value of "new quadruple" therapy in patients with chronic heart failure.

Purpose: This study aimed to evaluate the effectiveness of the "new quadruple" therapy in chronic heart failure (CHF) patients with metabolic syndrome using 2D speckle tracking imaging (2D-STI) stratified strain imaging to measure endocardial longitudinal strain while exploring its underlying neuroendocrine mechanisms.

Patients And Methods: The study retrospectively analyzed 158 patients with heart failure with reduced ejection fraction [HFrEF; left ventricular ejection fraction (LVEF) < 40%] treated with the "new quadruple" therapy (angiotensin receptor neprilysin inhibitor (ARNI), sacubitril/valsartan, dapagliflozin, bisoprolol, and spironolactone) for 8 weeks. Conventional ultrasound indices, left ventricular global longitudinal strain (LVGLS), and subendocardial longitudinal strain (LS) were measured pre- and post-treatment.

HAS-CIRCpedia-5280 sponges miR-4712-5p inhibited colon cancer autophagyinduced by human beta-defensin-1.

Background: Among all malignancies, colorectal cancer ranks third in incidence rate and second in mortality rate. Human beta-defensin-1 (hBD-1) has broad-spectrum antimicrobial properties, and it plays an important role in the tumor microenvironment. Circular ribonucleic acids (circRNAs) regulate the proliferation and progression of colorectal cancer cells via cancer-related signaling pathways.

Injectable celastrol-loading emulsion hydrogel for immunotherapy of low-immunogenic cancer.

Immunotherapy, exemplified by immune checkpoint blockade (ICB), has been extensively employed in antitumor treatments. Nevertheless, its efficacy in addressing low-immunogenic tumors has not yielded satisfactory results, primarily due to the depletion and inadequate infiltration of effector T cells within the tumor microenvironment (TME). Here, we construct an injectable water-in-oil emulsion hydrogel to load clinically used Celastrol (Gel@Cel), which addresses the limitations of Cel's hydrophobicity.

Mutation in Resilin reveals attachment impairment in Bombyx mori.

Insects can effortlessly walk on various substrate surfaces using attachment pads on their feet, even upside down on smooth surfaces. These pads undergo frequent deformation throughout the lifecycle of insects to achieve stable adhesion. Interest in insect adhesion spans 100s of years; studies have indicated the special mechanical properties and intricate structural design of insect attachment pads.

ESM-1 Promotes the Process of Diabetic Nephropathy by Promoting the Expression of CXCL3.

Background: The analysis of diabetic nephropathy (DN)-related gene dataset demonstrated that C-X-C motif chemokine ligand 3 (CXCL3) is highly expressed in DN. Exploring the impact of CXCL3 in the course of DN is the core goal of this study.

Methods: The cell model used in this study was CIHP-1 cells induced by high glucose (HG).

Synergistic effect of corn straw and mulberry leaves fermantation in vitro and effects on growth performance and antioxidant capacity of sheep.

This study was to determine the synergistic effect of corn straw and mulberry leaves in sheep diet. Corn straw and mulberry leaves were mixed as culture substrate for six groups: 100:0 (T0), 80:20 (T20), 60:40 (T40), 40: 60 (T60), 20:80 (T80) and 0:100 (T100). The in vitro culture indicated that mulberry leaves exhibited a positive correlation with theoretical gas production (GP), cumulative GP, microbial crude protein concentration, and organic matter digestibility.

Health risk assessment to xenoestrogen through atmospheric PM particles: A case study in Suzhou.

Xenoestrogens, classified as endocrine disruptors, can be inhaled through atmospheric particles, leading to adverse health effects such as cancer and developmental abnormalities. This research focused on analysing the monthly distribution, seasonal variation, and health impacts of six target xenoestrogens (dibutyl phthalate (DBP), di(2-ethylhexyl) phthalate (DEHP) and diisobutyl phthalate (DIBP)), bisphenol A (BPA), and alkylphenols (nonylphenol (NP) and 4-tert-octylphenol (4-t-OP)) in atmospheric PM at campus of Xi'an Jiaotong-Liverpool University from September 2021 to September 2023. The monthly average concentration of xenoestrogens was measured at 20 ng·m, while the mass concentration of PM varied between 1.

Pea Peptides and Heavy Metal Neurotoxicity: Exploring Mechanisms and Mitigation Strategies in PC12 Cells.

Calsyntenin-1 (Clst1) is a sensitive indicator of lead (Pb) toxicity in neural tissue. This study was designed to investigate the impact of lead exposure on Clst1 expression in PC12 cells and the mitigating effect of pea peptide 4 (PP4) on lead-induced neurotoxicity. Data showed that lead exposure, at varying doses and durations, disrupted the mRNA expression and protein levels of Clstn1 in PC12 cells.

High-Performance Fatigue-Resistant Dual- Polyrotaxane Hydrogel Electrolytes for Flexible Aqueous Zinc-Ion Batteries.

This study presents a novel anti-fatigue hydrogel electrolyte with a slip-ring structure for next-generation flexible wearable energy storage systems. Conventional quasi-solid aqueous zinc-ion batteries (ZIBs) with hydrogel electrolytes often suffer from mechanical degradation under repeated stress, limiting practical use. To overcome this, a dual-Polyrotaxane (DPR)-polyacrylic acid (PAA) hydrogel with a unique slip-ring architecture is synthesized, that enhances mechanical durability, self-healing, and adhesion.

Constructing a Nursing Programme to Manage Intra-Abdominal Pressure in Neurocritical Care Patients: A Modified Delphi Study.

Aim: To construct a nursing programme to manage intra-abdominal pressure (IAP) in neurocritical care patients.

Design: The consensus of 16 experts was collected using a two-round Delphi method.

Methods: First, we developed the early stages of the nursing programme for managing IAP in neurocritical care patients through preliminary study and relevant literature internationally.

Dissecting biological heterogeneity in major depressive disorder based on neuroimaging subtypes with multi-omics data.

The heterogeneity of Major Depressive Disorder (MDD) has been increasingly recognized, challenging traditional symptom-based diagnostics and the development of mechanism-targeted therapies. This study aims to identify neuroimaging-based MDD subtypes and dissect their predominant biological characteristics using multi-omics data. A total of 807 participants were included in this study, comprising 327 individuals with MDD and 480 healthy controls (HC).

Interacting MeZFP29 and MebZIPW regulates MeNRT2.2 from cassava responding to nitrate signaling.

Cassava is a significant tropical cash crop. MeZFP29 interacting with MebZIPW improves MeNRT2.2, encoding a high-affinity nitrate transporter, through binding to NREs under low nitrate and shows a nitrate-signaling-triggered regulation.