Isolation and Structure Elucidation of the Heterocumulene Anions [NCC-L]- (L = CO, CS, N2).

Cumulenes are molecules characterized by a series of consecutive double bonds. They serve as important reagents and intermediates in the synthesis of polymers and a wide variety of functionalized compounds, including various heterocycles. Understanding the properties of cumulenes and developing synthetic routes to these often highly reactive species is essential for unlocking new applications.

Spectroscopy and Bonding Analysis of ArBO ( = 1-3) Cations That Possess Argon-Boron Multiple Bonds.

ArBO ( = 1-3) complexes have been prepared and subjected to spectroscopic characterization in the gas phase. Mass-selected infrared photodissociation spectroscopy, in combination with theoretical calculations, reveals the coexistence of two nearly isoenergetic structural isomers in ArBO. One isomer entails two equivalent Ar atoms chemically bound to BO, while the other features an ArBO core ion accompanied by a weakly tagging argon atom.

Combination drug therapy prevents CIAKI by suppressing ER stress-induced apoptosis.

Contrast-induced acute kidney injury (CIAKI) is an important clinical complication that occurs after the application of contrast agent in percutaneous coronary intervention. The pathogenesis of CIAKI is complex. Studies have shown that cell apoptosis induced by endoplasmic reticulum stress (ERS) plays an important role in renal tubular injury in CIAKI.

Neutral 4π-electron tetrasilacyclobutadiene contains unusual features of a Möbius-type aromatic ring.

The search for stable compounds containing an antiaromatic cyclic 4π system is a challenge for inventive chemists that can look back on a long history. Here we report the isolation and characterization of the novel 4π-electron tetrasilacyclobutadiene, an analogue of a 4π neutral cyclobutadiene that exhibits surprising features of a Möbius-type aromatic ring. Reduction of RSiCl (R = (Pr)PCH) with KC in the presence of cycloalkyl amino-carbene (cAAC) led to the formation of corresponding silylene 1.

Inflammatory Markers and Measures of Cardiovascular Autonomic Neuropathy in Type 1 Diabetes.

Background: Cardiovascular autonomic neuropathy (CAN) and inflammation predict more severe outcomes in type 1 diabetes (T1D). However, the link between CAN and inflammation in T1D remains unclear. We examined associations between CAN measures and inflammatory biomarkers in individuals with T1D.

Copper-Catalyzed Asymmetric Nucleophilic Opening of 1,1,2,2-Tetrasubstituted Donor-Acceptor Cyclopropanes for the Synthesis of α-Tertiary Amines.

Catalytic asymmetric transformation of donor-acceptor cyclopropanes (DACs) has been proven to be a highly valuable and robust strategy to construct diverse types of enantioenriched molecules. However, the use of 1,1,2,2-tetrasubstituted DACs to form products bearing quaternary stereocenters remains a long-term unsolved challenge. Here, we report the copper-catalyzed asymmetric aminative ring opening of tetrasubstituted alkynyl DACs that delivers a myriad of α-tertiary amines with high levels of enantioselectivities.

A Systematic Method to Detect Next-Generation Sequencing-Based Microsatellite Instability in Plasma Cell-Free DNA: plasmaMSI.

Microsatellite instability (MSI) detection using tumor tissue is a well-established prognostic and predictive biomarker for certain types of cancers. However, tumor tissue samples are less convenient to obtain than blood plasma samples. The main challenge facing next-generation sequencing-based MSI detection in blood plasma samples is the ultralow signal/noise ratio in plasma cell-free DNA (cfDNA).

Visual codon: a user-friendly Python program for viewing and optimizing gene GC content.

Due to the codon bias of different species, codon optimization is usually carried out in the process of heterologous protein expression. At present, there are a variety of codon optimization tools. However, the optimized sequences may still have high or low points of local guanine and cytosine (GC) content, which is not conducive to the primer design of gene subcloning, and also makes it difficult to perform the experiment of synthesizing the whole gene with DNA fragments by polymerase chain reaction (PCR) reaction.

SP1/COL1A2/ZEB1 axis promotes TGF-β2-induced lens epithelial cell proliferation, migration, invasion and EMT process.

Posterior capsule opacification (PCO) is the most common complication after cataract surgery. In this study, we used transforming growth factor beta-2 (TGF-β2)-induced SRA01/04 cells to mimic PCO cell model and explored the functions and underlying mechanisms of specific protein 1 (SP1) in TGF-β2-induced SRA01/04 cell development. MTT assay and EdU assay were carried out to explore the proliferation of SRA01/04 cells.

Research progress on fermentation-produced plant-derived bioactive peptides.

With the advancement of biotechnology and the human pursuit of a healthy lifestyle, investigations on bioactive peptides (BAPs) have received increasing attention. Compared to proteins, BAPs have lower molecular weights and are more easily digested and absorbed by the human body, exhibiting various physiological functions. For instance, they can inhibit the angiotensin-converting enzyme, lower blood pressure, reduce cholesterol, and possess antioxidant, antimicrobial, and antiviral properties.

AcGLK1 promotes chloroplast division through regulating AcFtsZ1 in Actinidia chinensis.

This study unravels a new regulatory member (AcGLK1) that regulates chloroplast division by affecting the expression levels of cytoskeletal filamenting temperature-sensitive Z (FtsZ) in Actinidia chinensis. GOLDEN 2-LIKE (GLK) transcription factor members of GARP subfamily play an irreplaceable role in regulating chloroplast biogenesis and development. Here we report the functional characterization of a novel GLK1 homolog (AcGLK1) isolated from kiwifruit (Actinidia chinensis cultivar 'Hongyang').

STT3-mediated aberrant N-glycosylation of CD24 inhibits paclitaxel sensitivity in triple-negative breast cancer.

Paclitaxel is one of the main chemotherapic medicines against triple-negative breast cancer (TNBC) in clinic. However, it has been perplexed by paclitaxel resistance in TNBC patients, resulting in a poor prognosis. Abnormal protein glycosylation is closely related to the occurrence and progression of tumors and malignant phenotypes such as chemotherapy resistance.

Dual-mode detection of human immunoglobulin via copper oxide nanozyme catalysis fluorescent species generation and photoelectrochemical alteration in ZnInS/SnO-based system.

Human immunoglobulin (HIgG) has gained recognition as a crucial biomarker diagnosing and treating various diseases, particularly in identifying elevated serum levels in conditions like measles and pneumococcal disease. Traditional detection methods, however, are often hindered by inefficiencies, high costs, and potential inaccuracies, underscoring the urgent need for more sensitive, efficient, accurate, and self-calibration methods for HIgG. Here, a novel ZnInS/SnO composites was synthesized, featuring uniformly dispersed SnO nanoparticles on the flower-like ZnInS structure, resulting in a type II heterojunction that promotes the separation and transfer of photogenerated carriers.

Effects of Rocky Desertification Stress on Oat ( L.) Seed Germination and Seedling Growth in the Karst Areas of Southwest China.

Oat is an important crop widely distributed in temperate zones and is also commonly planted in the karst areas of southwest China. However, due to severe rocky desertification, the complex soil in this area is characterized by high calcium content, alkaline conditions, and drought, which significantly negatively impact the growth of oat seedlings. To study the adaptability of oats to rocky desertification stress at the seedling stage, we investigated the effects of CaCl (0-150 mM), the pH (3-9), and drought stress (PEG-6000 solution at 0 to -0.

Prognostic value of central gland volume on MRI for biochemical recurrence after prostate radiotherapy.

Purpose: This study evaluates pretreatment prostate magnetic resonance imaging (MRI) metrics and clinical characteristics in predicting biochemical recurrence (BCR) after prostate radiotherapy (RT).

Methods: In this retrospective single institution study, we identified men in our prostate cancer database who underwent MRI within 6 months prior to completing definitive RT from May 2011 to February 2023. Central gland volume and peripheral zone volume were measured by a radiologist using manual segmentation, along with Prostate Imaging-Reporting and Data System (PI-RADS) score.

Assessing Students' Critical Thinking in Dialogue.

Critical thinking has been widely considered an important skill in the 21st century. In view of the value attached to critical thinking, various quantitative instruments have been developed to assess critical thinking, which only provide a product of critical thinking and cannot reveal the critical thinking process of test takers. Hence, this paper proposes a coding scheme facilitating a qualitative analysis of critical thinking exhibited in interaction.

Clinical characteristics of patients with variant-associated epilepsy and therapeutic exploration: a case report and literature review.

The gene encodes a transmembrane prolyl 4-hydroxylase, which is responsible for the degradation of hypoxia-inducible transcription factors (HIF) under normoxia. Clinically, biallelic variants have been identified in patients with hypotonia, hypoventilation, intellectual disabilities, dysautonomia, epilepsy, and eye abnormalities (HIDEA syndrome). Seizure was one of the most prominent symptoms.

Purification and Characterization of γ-Glutamyl Transpeptidase from and Its Function on Formation of Sulfur-Containing Volatiles.

γ-Glutamyl transpeptidase (GGT) in shoot (TS) was extracted and purified through salt precipitation, ion-exchange column, and gel filtration chromatography methods. The GGT had two peptides with M.W.

Understanding the interplay of compassion fatigue and moral resilience on moral distress in ICU nurses: a cross-sectional study.

Background: Intensive Care Unit (ICU) nurses frequently confront significant psychological challenges, including compassion fatigue, moral distress, and diminished moral resilience. These issues not only affect their well-being but also impact the quality of care provided to patients. The interplay of these factors is complex and not fully understood, particularly how compassion fatigue influences the relationship between moral resilience and moral distress.

Assessing acute kidney injury risk after COVID vaccination and infection in a large cohort study.

Acute kidney injury (AKI) has been noticed after both COVID-19 vaccination and infection, affecting risk-benefit evaluations and vaccine hesitancy. We conducted a large-scale N3C cohort study to compare AKI incidence following COVID-19 vaccination and infection. Participants from December 2020 to August 2023 were divided into two groups based on their initially observed COVID-19 antigen exposure: COVID-19 vaccination group (n = 2,953,219) and COVID-19 infection group (n = 3,616,802).

Prognostic impact of age on outcomes of hepatic decompensation in patients with compensated cirrhosis (CHESS2102): an international, multicenter cohort study.

Baveno VII criteria (B7C) and Baveno VI criteria (B6C) have been widely used to estimate the risk of hepatic decompensation. However, the impact of age on these criteria warrants further investigation. The international, multicenter cohort study included 1138 patients with compensated cirrhosis (median follow-up of 40.