Rapid and Visual Detection of in Blood Samples from Pet Cats and Dogs by Loop-Mediated Isothermal Amplification.

is an obligate intracellular parasite that invades nearly all nucleated cells of a broad spectrum of vertebrate hosts, and which may cause serious disease in immunocompromised patients, as well as in the immunologically incompetent fetus. This study aimed to establish a loop-mediated isothermal amplification (LAMP) technique to rapidly detect in the blood infection by targeting the 529 bp repeat element of . A turbidity monitoring system, together with visual reagent, was used to test the amplification result of the LAMP assay.

Glass transitions in frozen systems as influenced by molecular weight of food components.

Freezing is a frequently used way to expand the storage life of foods with high water content. Under suitable cooling rates, frozen systems attain a condition of maximum freeze concentration, which is characterized by the glass transition temperature (T '), end point of freezing or onset of melting (T '), and concentration of solids (X ') in the maximum-freeze-concentrated matrix. The value of T ', T ', and X ' depends on the chemical composition of frozen system.

[Effects of five Chinese herbs on human demodicid mites ].

Objective: To research the effects of five Chinese herbs of , pine needles and on human demodicid mites .

Methods: The human demodicid mites were acquired with the cellophane tape method. The ethanol heat reflux extractions were carried out on the 5 kinds of Chinese herbs to be studied and as the positive control, so as to get the herbal extracts.

[Investigation on Demodex infection status and influencing factors in medical students in Wuhu City].

Objective: To investigate the Demodex infection status and influencing factors in medical students in Wuhu City.

Methods: The mite specimens were collected by the cellophane tape method, and the influencing factors were investigated through the questionnaires.

Results: A total of 316 medical students were surveyed, 117 students were infected with Demodex mite, and the total infection rate was 37.

Influences of PON1 on airway inflammation and remodeling in bronchial asthma.

This study aims to explore the influences of Paraoxonase-1 (PON1) involved in airway inflammation and remodeling in asthma. Mice were divided into control, asthma, asthma + PON1 and asthma + NC groups, and asthma models were established via aerosol inhalation of ovalbumin (OVA). HE, Masson, and PAS stains were used to observe airway inflammation and remodeling, Giemsa staining to assess inflammatory cells in bronchoalveolar lavage fluid (BALF), qRT-PCR and Western blot to detect PON1 expression, lipid peroxidation and glutathione assays to quantify malondialdehyde (MDA) activity and glutathione peroxidase (GSH) levels, ELISA to determine inflammatory cytokines and immunoglobulin, and colorimetry to detect PON1 activities.

Effect of osmotic dehydration pretreatment and glassy state storage on the quality attributes of frozen mangoes under long-term storage.

Changes in the quality of frozen mango cuboids were investigated during long-term glassy state storage with and without osmotic dehydration pretreatment. The mango cuboids were dehydrated in mixed solutions (sucrose: glucose: fructose in a ratio of 3.6:1:3) of different concentrations (30, 40, and 50% (wt/wt)) prior to freezing and then stored at -55 °C (in the glassy state) for 6 months.

Investigation on the zoonotic trematode species and their natural infection status in Huainan areas of China.

Background: To investigate the species of zoonotic trematodes and the endemic infection status in the domestic animals in Huainan areas, north Anhui province of China, we intent to provide evidences for prevention of the parasitic zoonoses.

Methods: The livestock and poultry (definitive hosts) were purchased from the farmers living in the water areas, including South Luohe, Yaohe, Jiaogang and Gaotang Lakes, and dissected the viscera of these collected hosts to obtain the parasitic samples. Then the specimens were microscopically identified, with reference to the descriptions in previous literatures for counting the zoonotic species found in these areas.

[Exploration on the Technology of Counter Staining of Fluke].

Fixed samples of Clonorchis sinensis and Fasciolopsis buski were stained with acetocarmine and malachite green, or stained with acetocarmine only. The samples displayed three different colors after staining with acetocarmine and malachite green. The digestive system, excretory system and the surrounding muscle tissue were stained reddish, the uterus was bright green, and the vitellarium at each side of the worm was tan.

[Investigation on schistosomiasis cognitive levels of people from Chaohu area].

Objective: To investigate the cognitive levels of people from Chaohu area, Anhui Province about the prevention and control knowledge of schistosomiasis before the water transfer project operation, so as to provide the reference for formulating the health education intervention.

Methods: A schistosomiasis questionnaire survey was conducted among the students, fishermen, boat people and villagers from Chaohu selected by the random cluster sampling from May to October, 2014.

Results: A total of 1 140 questionnaires were issued with the actual recovery of 1 096 copies and 1 032 valid questionnaires.

[Survey and morphological observation of in college canteens].

Objective: To understand the breeding and morphology of in college canteens in south Anhui Province.

Methods: The powder and rice on the floor around dough makers, flour bags and rice bags in college canteens were collected and observed under a microscope.

Results: Totally 108 samples were collected, and Acaroid mites were found in 101 of them with a detection rate of 93.

[Metorchis orientalis founded in basin of Wuhu City, Anhui Province].

Objective: To investigate the epidemic situation of Metorchis orientalis in Wuhu City, Anhui Province.

Methods: Three lakes (Fengming, Longwo and Kui lakes) were selected in Wuhu City, and the poultries around the lakes and freshwater fishes in the lakes were captured to test the infection of M. orientalis, and the infection rates were calculated.

Purification and structural analysis of membrane-bound polyphenol oxidase from Fuji apple.

Membrane-bound polyphenol oxidase (mPPO) in Fuji apple (Malus domestica Borkh. cv. Red Fuji) was purified and analyzed with a nanoelectrospray ionization mass spectrometer.

Comparison of membrane-bound and soluble polyphenol oxidase in Fuji apple (Malus domestica Borkh. cv. Red Fuji).

This study compared membrane-bound with soluble polyphenol oxidase (mPPO and sPPO, respectively) from Fuji apple. Purified mPPO and partially purified sPPO were used. mPPO was purified by temperature-induced phase partitioning and ion exchange chromatography.

Dujardinascaris gigantea sp. n. (Nematoda: Ascaridida) from the critically endangered crocodile Alligator sinensis Fauvel (Reptilia: Crocodylia).

The Chinese alligator Alligator sinensis Fauvel (Reptilia: Crocodylia) is considered as one of the most critically endangered species of the 23 extant crocodiles. However, our knowledge of the helminth parasites of this rare animal is completely lacking. During a helminthological survey of reptiles in China, we found a new ascaridoid nematode, Dujardinascaris gigantea sp.

[Zoonotic trematode species identified in domestic animals from Huainan area].

Objective: To understand the zoonotic trematode species identified in domestic animals from Huainan area, Anhui Province, so as to supply the evidence for the prevention of zoonoses.

Methods: The livestock/definitive hosts were commercially available on category basis, and sacrificed for obtaining the parasitic samples via dissecting the viscera. The specimens were microscopically identified, with reference to the descriptions in the previous literature.

[Investigation on species and community ecology of cheyletoid mites breeding in the stored traditional Chinese medicinal materials].

Objective: To investigate the species of Cheyletoid mites breeding status in the stored traditional Chinese medicinal materials and the relationship between its community and habitats.

Methods: A total of 60 samples of the traditional Chinese medicinal materials were collected from Huainan, Wuhu and Xuancheng in China. The mites were isolated with shakesieve shock, directicopy, Tullgren funnel, waternacopy and redricopy, and identified and counted under the light microscope.

[Reform and practice on the experiment teaching of medical parasitology].

A new model of education is investigated to meet the new idea of experiment teaching in university. Therefore the establishment of experiment teaching model of medical parasitology needs to be correspondently reformed. A variety of new management measures are taken to raise the efficiency of experiment teaching in training the students in the College.

[Effect of Sry silencing by siRNA on the expression of sex determining genes in mouse embryos].

In order to investigate Sry regulation network in the development of male embryo, we inhibited the Sry gene expression by RNAi and then examined expression of other sex-related genes. Six genes (Sox9, Wt1, Sf1, Dax1, Gata4 and Amh), which are suggested to be closely related to Sry regulation were studied. Two siRNA expression vectors pSilencer4.

Expression of CXC chemokine IP-10 in patients with chronic hepatitis B.

Background: Chemokines have strong chemoattractant effects and are involved in a variety of immune and inflammatory reactions, such as attracting activated T lymphocytes, neutrophils, monocytes and natural killer cells via the pathway of G protein-coupled receptors to sites of inflammatory injury and contribute to wound repair. This investigation was designed to assess the levels of chemokine interferon-gamma inducible protein-10 (IP-10) and IP-10 mRNA, and the relationship between IP-10 mRNA and HBV-DNA and alanine aminotransferase (ALT) in patients with chronic hepatitis B.

Methods: The levels of IP-10 mRNA in peripheral blood mononuclear cells (PBMCs) were kinetically detected by real-time polymerase chain reaction (PCR).

[Impact of reproductive hormone on mouse embryo sexes].

The effects of reproductive hormone on mouse embryo sexes were studied with in vitro fertilization (IVF), in vitro culture (IVC) and sex identification. Results showed that it had no adverse effects on embryos cleavage and development after the embryos were incubated with the fertilization media with 17beta-E2, FSH and progesterone respectively at the concentration of this study. Mice embryos sex ratios significantly deviated from 1:1(P < 0.

[Effect of fertilization condition on mammalian sex determination].

Selection of sex at or before conception is perhaps the most desired biotechnology of all time because many economic traits are related with sex. Sex selection by manipulating the conditions of sperm-oocytes fertilization would be one of the most practicable and widely used methods. This review summarized the progress in the effects of fertilization conditions on mammalian sex.