mutations in with negative MPT64 antigen assay results from a tertiary hospital in Southeastern China.

Background: MPT64 protein is an effective marker for detecting (MTB) in liquid culture and clinical tissue samples. However, some MTB clinical isolates test negative for this antigen because of varied mutation types across different regions.

Methods: DNA samples of MPT64 antigen assay-negative MTB strains were collected from a tertiary hospital from January 2016 to January 2024, and gene mutations were detected by sequencing.

Strain-Modulated Deposition Mechanism on a Flexible Zinc Anode.

Flexible aqueous zinc-ion batteries (AZIBs) are considered one of the most attractive flexible devices owing to their high theoretical capacity, low cost, and high security. However, the formation of Zn dendrites and the poor flexibility of the Zn material greatly impede the application of wearable AZIBs. Herein, by transferring graphene onto the surface of polyethylene terephthalate-indium tin oxide (PET-ITO-G), a substrate combining excellent flexibility and dendrite suppression ability was prepared.

Integrating transcriptomics and Microbiomics to unravel the regulatory effects of Anji white tea on lipid metabolism in HFD-induced obese mice.

Obesity has become a significant global health concern. Green tea is widely consumed as an internationally popular beverage and it is recognized that various green teas have unique biological activities. Here, the effects of Anji white tea (a green tea) ‌on lipid metabolism in high-fat-diet (HFD)-fed mice were investigated based on multi-omics.

Assessing the performance of QLQ-C30 in predicting all-cause mortality in community cancer patients.

Background: To evaluate the ability of QLQ-C30 in predicting mortality in community-based cancer patients in China.

Methods: A whole-cluster sampling method was adopted to enroll cancer patients in four communities in Shanghai from 2018 to 2019. The patients were surveyed using a questionnaire enquiring demographic information, cancer types, and QLQ-C30 scale.

Structural interactions and mechanisms of amylose and amylopectin binding with cyanidin-3-O-glucoside.

In this study, the structural interactions between starch and cyanidin-3-O-glucosde (C3G) were evaluated using three different starches with an amylose content of 20 % (CS) and 60 % (HS) and amylopectin content of 99 % (WS). It also increased the fractal dimension D values of CS, HS and WS from 1.83, 1.

[Effects of p38 phosphorylation on stemness maintenance and chemotherapy drug resistance of PANC-1 cells].

Objective The aim of this study was to investigate the effect of p38 on stem cell maintenance of pancreatic cancer. Methods Human pancreatic cancer cells PANC-1 were treated with different concentrations of 5-fluorouracil(5-FU)(0.5×IC, IC, and 2×IC) for 24 hours, and VX-702 (p38 phosphorylation inhibitor) was added, and the cells were inoculated in 6-well culture dishes with ultra-low adhesion to observe the changes of sphere tumors.

Wearable Devices as Tools for Better Hypertension Management in Elderly Patients.

BACKGROUND Hypertension is a growing global health concern, particularly among the elderly, and is a significant contributor to cardiovascular and cerebrovascular diseases. Managing hypertension in elderly patients is particularly challenging, requiring continuous monitoring and strict adherence to treatment protocols. This study aimed to evaluate the effectiveness of integrated management strategies that utilize wearable devices in enhancing hypertension management for elderly patients.

Role of MLIP in burn-induced sepsis and insights into sepsis-associated cancer progression.

Introduction: Burn-induced sepsis is a critical clinical challenge marked by systemic inflammation, immune dysregulation, and high mortality. Macrophage-driven inflammatory pathways are central to sepsis pathogenesis, while immune cell metabolic reprogramming plays a key role in both sepsis and cancer progression.

Methods: Bioinformatics analyses using GEO, TCGA, and GTEx datasets identified MLIP-modulated genes linked to immune responses and prognosis.

Expert consensus on intentional tooth replantation.

Intentional tooth replantation (ITR) is an advanced treatment modality and the procedure of last resort for preserving teeth with inaccessible endodontic or resorptive lesions. ITR is defined as the deliberate extraction of a tooth; evaluation of the root surface, endodontic manipulation, and repair; and placement of the tooth back into its original socket. Case reports, case series, cohort studies, and randomized controlled trials have demonstrated the efficacy of ITR in the retention of natural teeth that are untreatable or difficult to manage with root canal treatment or endodontic microsurgery.

Pectin-coated Malvidin-3-O-galactoside attenuates silica-induced pulmonary fibrosis by promoting mitochondrial autophagy and inhibiting cell apoptosis.

Background: Blueberries are a rich source of anthocyanins, which have been established to have multiple beneficial properties. However, the structure of anthocyanin monomers is unstable and their bioavailability is low. To date, whereas functional studies on anthocyanins have focused mainly on the effects of their monomers on liver and kidney, few have examined the interventional effects on pulmonary fibrosis.

Electronic Structure Tunable Metallosupramolecular Polymers as Bifunctional Electrocatalysts for Rechargeable Zn-Air Battery.

Metallosupramolecular polymers (MSPs) have shown great potential in the area of oxygen electrocatalysis due to their tunable electronic structure, and predictable coordination environment. Further exploration of structure-performance relationships of oxygen electrocatalysts is crucial for designing highly efficient catalysts. Herein, a strategy is proposed to prepare MSP-based bifunctional oxygen electrocatalysts with different oxygen electrocatalytic preferences (Co-AQ and Co-AN) by adjusting the electronic structure of organic linkers.

Tailoring Octahedron-Tetrahedron Synergism in Spinel Catalysts for Acidic Water Electrolysis.

The instability issues of oxide-based electrocatalysts during the oxygen evolution reaction (OER) under acidic conditions, caused by the oxidation and dissolution of the catalysts along with the current-capacitance effect, constrain their application in proton exchange membrane water electrolysis (PEMWE). To address these challenges, we tailored the spinel structure of CoO and exploited the synergism between the tetrahedron and octahedron sites by partially substituting Co with Ni and Ru (denoted as NiRuCoO), respectively. Such a catalyst design creates a Ru-O-Ni electronic coupling effect, facilitating a direct dioxygen radical-coupled OER pathway.

Combining an in situ device fabrication and six-probe electrical transport measurement system with low-energy electron microscopy.

Two-dimensional (2D) quantum materials, including several analogs of graphene ("X-enes"), are of great current research interest. However, some of the potentially most exciting ones are reactive and sensitive to exposure to the atmosphere, which hampered the experimental study of their key physical properties. Here, we introduce an experimental setup that integrates sub-atomic-layer-resolved molecular beam epitaxy (MBE) synthesis, real-time low-energy electron microscopy (LEEM) and low-energy electron diffraction (LEED), and in situ six-probe electrical transport measurements.

Exploring information technology utilization and needs in community pharmacies: a cross-sectional survey in Shanghai, China.

Background: Community pharmacists are critical in managing medication therapy and ensuring patient safety. However, in many countries, including China, challenges such as limited access to real-time patient data, fragmented communication systems, and underdeveloped clinical decision-support tools hinder the effectiveness of pharmacy services. Integrating information technology (IT) into community pharmacies can improve medication management by providing timely access to patient information, enhancing medication safety, and facilitating communication with healthcare providers.

Decrease in Escherichia-Shigella in the gut microbiota of ESKD patients undergoing maintenance hemodialysis.

Background: Gut dysbiosis is thought to be involved in the pathogenesis and progression of chronic kidney disease and end-stage kidney disease (ESKD). However, differences in the composition and function of gut microbiota in hemodialysis patients are not consistently concluded.

Methods: A total of 20 patients receiving maintenance hemodialysis (MHD) treatment at the Blood Purification Center of Bethune International Peace Hospital from March 2021 to December 2022 were included based on the inclusion criteria.

Preparation of dual-epitopes imprinted particles with γ-cyclodextrin host-guest interaction and reversible addition-fragmentation chain transfer strategy for cytochrome c collaborative recognition.

A dual-epitopes imprinted strategy for cytochrome c selective recognition assisted with γ-cyclodextrin host-guest interaction via N-terminal and C-terminal epitope's simultaneous imprinting and reversible addition-fragmentation chain transfer (RAFT) polymerization was developed. N-terminal and C-terminal nonapeptides of Cyt c (GI-9 and AE-9) were used simultaneously as the epitope to achieve collaborative recognition for cytochrome c. As a supramolecule, γ-cyclodextrin can encapsulate the aromatic functional groups of amino acid residues to capture the peptide and improve the corresponding spatial orientation for epitope or cytochrome c recognition by host-guest interaction.

Study on the mechanism of decomposition of methane hydrate by the compound inhibitor.

Gas hydrate plugs, slow dissociation rates, and low production have long posed significant challenges to the commercial viability of gas hydrate extraction. This study investigated the inhibitory effects of ethylene glycol (EG), EG + polyvinyl pyrrolidone (PVP), and EG + PVP + sodium chloride (NaCl) on the dissociation characteristics of methane hydrate through molecular dynamics simulations and experiment. Simulation results indicate that the hydroxyl groups in ethylene glycol (EG) molecules and chloride ions of sodium chloride (NaCl) can effectively form hydrogen bonds with the water molecules in the hydrate and disrupt the cage structure of the methane hydrate, accelerating the dissociation of the hydrate.

Interleukin-38 as a potential diagnostic biomarker for heart failure.

Objective: Interleukin-38 (IL-38) attenuates inflammation, myocardial injury, and ventricular remodeling after myocardial infarction, which potentially reduces the incidence of heart failure (HF). This study aimed to evaluate the diagnostic value of IL-38 for HF.

Methods: IL-38 was detected via enzyme-linked immunosorbent assay in the serum samples of 238 hF patients and 30 healthy controls when enrollment.

Biofunctional supramolecular injectable hydrogel with spongy-like metal-organic coordination for effective repair of critical-sized calvarial defects.

In clinical settings, regenerating critical-sized calvarial bone defects presents substantial problems owing to the intricacy of surgical methods, restricted bone growth medications, and a scarcity of commercial bone grafts. To treat this life-threatening issue, improved biofunctional grafts capable of properly healing critical-sized bone defects are required. In this study, we effectively created anti-fracture hydrogel systems using spongy-like metal-organic (magnesium-phosphate) coordinated chitosan-modified injectable hydrogels (CPMg) loaded with a bioinspired neobavaisoflavone (NBF) component.

Anatomical Measurements of the Thoracic Splanchnic Nerve and Clinical Applications.

Objective: To describe in detail the characteristics of the travels and relationship of splanchnic nerves, and to provide an anatomical basis for splanchnic nerve block.

Methods: 1) For 20 thoracic splanchnic nerve anatomies, the relationship between the intersection of the greater splanchnic nerve (GSN) and lesser splanchnic nerve (LSN) and the T12 vertebrae was examined. 2) 6 patients with cancer pain in the epigram, unresponsive to conservative treatment, underwent splanchnic nerve radiofrequency ablation.

Galvanic replacement mediated morphological adjustments boost nanoparticle performance in electrocatalytic alcohol oxidation.

The PdPtCo core-shell nanostructure, fabricated galvanic replacement by rapidly incorporating Pd into PtCo nanoalloys at room temperature, excels in alcohol oxidation (AOR). Synergistic effects lower Pt electron density, promoting C-C cleavage, while increased Co coverage reduces CO adsorption, enhancing CO tolerance. This work offers a novel strategy for high-performance AOR catalysts.

In vitro simulated digestion and fermentation characteristics of polyphenol-polysaccharide complex from Hizikia fusiforme and its effects on the human gut microbiota.

This study investigated the effects of the polyphenol-polysaccharide complex (HPC) and its purified components (PC1 and PC4), obtained from Hizikia fusiforme, on the human gut microbiota during in vitro simulated digestion and fecal fermentation. Results showed a gradual increase in reducing sugar content for HPC, PC1, and PC4 during simulated digestion, accompanied by a slight decrease in molecular weight, indicating that these complexes were not completely digested during oral-gastrointestinal digestion. However, following fermentation, the molecular weights of HPC, PC1, and PC4 decreased significantly, and the molar ratios of monosaccharide compositions changed considerably compared with prefermentation values.

Pepper (Capsicum annuum L.) AP2/ERF transcription factor, CaERF2 enhances salt stress tolerance through ROS scavenging.

The AP2/ERF transcription factor CaERF2 in pepper enhanced salt tolerance by scavenging reactive oxygen species. The effects of salt stress on plant development and progression are substantial. Ethylene response factor transcription factors (TFs) play a crucial role in responses to salt stress.

Band Gap Narrowing in Lead-Halide Perovskites by Dynamic Defect Self-Doping for Enhanced Light Absorption and Energy Upconversion.

Metal halide perovskites (MHP) have attracted great attention in the photovoltaic industry due to their high and rapidly rising power conversion efficiencies, currently over 25%. However, hybrid organic-inorganic MHPs are inherently chemically unstable, limiting their application. All-inorganic MHPs perovskites, such as CsPbI, have many merits, but their stable conversion efficiency is lower, around 18%, due to a larger band gap causing a mismatch with the solar spectrum.