Publications by authors named "Zhanqing Fan"
Article Synopsis
- Researchers developed a new RNA base editor called compact and efficient RNA base editor (ceRBE) that replaces the larger dCas13 protein, improving its in vivo application capabilities.
- The ceRBE efficiently edits adenine-to-inosine (A-to-I) and cytidine-to-uridine (C-to-U) while minimizing off-target effects in HEK293T cells.
- In a humanized mouse model for Duchenne muscular dystrophy, ceRBE successfully repaired a mutation and restored gene expression, indicating its potential for treating genetic diseases.
Improved catalytic activity of a novel aspartate kinase by site-directed saturation mutagenesis.
Bioprocess Biosyst Eng
March 2022
This study aimed to improve the catalytic activity of aspartate kinase (AK), the first key rate-limiting enzyme in the aspartic acid metabolism pathway, by site-directed saturation mutagenesis, and to weaken the synergistic feedback inhibition of metabolites and analyze its mechanism using molecular dynamics simulation (MD). The key residual sites around the inhibitor lysine (Lys) were selected to construct the mutant strains. The mutant A380M with significantly increased enzyme activity was obtained through enzyme activity screening.
View Article and Find Full Text PDFIn microorganisms and plants, aspartate kinase (AK) is the initial committed enzyme of the biosynthesis of the aspartate acid family amino acids and is inhibited by end products. In the paper, we mutated the key allosteric regulatory site A380 around the binding site of the Lys inhibitor in AK (AK). A single-mutant A380C was obtained with 12.
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