Study on In-Situ Tool Wear Detection during Micro End Milling Based on Machine Vision.

Most in situ tool wear monitoring methods during micro end milling rely on signals captured from the machining process to evaluate tool wear behavior; accurate positioning in the tool wear region and direct measurement of the level of wear are difficult to achieve. In this paper, an in situ monitoring system based on machine vision is designed and established to monitor tool wear behavior in micro end milling of titanium alloy Ti6Al4V. Meanwhile, types of tool wear zones during micro end milling are discussed and analyzed to obtain indicators for evaluating wear behavior.

Evaluation of Three-Dimensional Surface Roughness in Microgroove Based on Bidimensional Empirical Mode Decomposition.

Micromilling is an extremely important advanced manufacturing technology in the micromanufacturing industry. Compared with the traditional milling process, micromilling has stricter requirements on the surface roughness of the workpiece, and the roughness of the microcurved surface is not easy to measure. In order to more accurately characterize the curved surface morphology of the microgrooves obtained by micromilling, this paper proposes a method to extract the reference plane of the curved surface based on the bidimensional empirical mode decomposition algorithm and characterize the three-dimensional surface roughness of the curved surface.

Laser-Induced Slippery Liquid-Infused Surfaces with Anticorrosion and Wear Resistance Properties on Aluminum Alloy Substrates.

Slippery liquid-infused surfaces (SLISs) are developed as a potential alternative to superhydrophobic surfaces (SHSs) to resolve the issues of poor durability in corrosion protection and wear resistance. In this work, we used a simple laser processing technology to prepare a SLIS on the aluminum alloy (7075) surface. The superhydrophobicities of the modified surface and the oil film formed by liquid injection make the corrosive medium difficult to directly contact the surface and thus have a significant effect on corrosion resistance.

Preparation of Micro-Pit-Textured PCD Tools and Micro-Turning Experiment on SiCp/Al Composites.

Serious tool wear occurs very often during machining due to the reinforcing phases in the workpiece. In this study, micro-pit-textures were prepared on the surfaces of PCD tools with a nanosecond laser to improve their cutting performance on SiCp/Al composites. The micro-pits were designed with rounded corners to improve the chip flow.

Regulated Li S Deposition toward Rapid Kinetics Li-S Batteries by a Separator Modified by CeO -Decorated Porous Carbon Nanostructure.

Although the high-energy-density lithium sulfur (Li-S) battery has been considered one of the most promising next-generation energy storage technology, the practical applications have been plagued by the sluggish reaction kinetics and the shuttle effect of lithium polysulfides intermediates. Here, to address the above issues, the authors report a novel separator modified by CeO -decorated porous carbon nanostructure (CeO /KB/PP). Benefiting from the strong polar surface and large specific surface area, (CeO -doped Ketjen Black) delivers efficient chemical adsorption toward lithium polysulfides.

A novel battery separator coated by a europium oxide/carbon nanocomposite enhances the performance of lithium sulfur batteries.

Lithium sulfur (Li-S) batteries represent one of the most promising future power batteries due to their remarkable advantages of low cost and ultrahigh theoretical energy density. However, the commercial applications of Li-S batteries have long been plagued by the shuttling effect of polysulfides and sluggish redox kinetics of these species. Herein, we designed a novel battery separator coated by a europium oxide-doped porous Ketjen Black (EuO/KB) and tested its performance for the Li-S batteries for the first time.

P4HA1 regulates human colorectal cancer cells through HIF1α-mediated Wnt signaling.

Colorectal cancer (CRC) is the third most commonly diagnosed malignancy that is associated with high levels of mortality. CRCs are often associated with an aberrant wingless-type mouse mammary tumor virus integration site family (Wnt) signaling pathway known to be responsible for tumorigenesis and cancer progression. Other factors that contribute to CRC pathology include hypoxia, extracellular matrix and cellular microenvironment.

Bio-Inspired Design of Bi/Tridirectionally Anisotropic Sliding Superhydrophobic Titanium Alloy Surfaces.

Many biological surfaces with the multi-scale microstructure show obvious anisotropic wetting characteristics, which have many potential applications in microfluidic systems, biomedicine, and biological excitation systems. However, it is still a challenge to accurately prepare a metal microstructured surface with multidirectional anisotropy using a simple but effective method. In this paper, inspired by the microstructures of rice leaves and butterfly wings, wire electrical discharge machining was used to build dual-level (submillimeter/micrometer) periodic groove structures on the surface of titanium alloy, and then a nanometer structure was obtained after alkali-hydrothermal reaction, forming a three-level (submillimeter/micrometer/nanometer) structure.

Transcription box‑3 protects human umbilical vein endothelial cells in a high‑glucose environment through sirtuin 1/AKT signaling.

The increasing burden of diabetes in low and middle‑income countries is attributable to both genetic and epigenetic factors. Environmental‑ and lifestyle‑associated changes are also considered to be important contributors to this disease. The resultant co‑morbidities arising from micro‑and macrovascular changes in diabetes are difficult to manage and are an economic burden.

Bioinspired Reversible Switch between Underwater Superoleophobicity/Superaerophobicity and Oleophilicity/Aerophilicity and Improved Antireflective Property on the Nanosecond Laser-Ablated Superhydrophobic Titanium Surfaces.

In this work, the bioinspired reversible switch between underwater superoleophobicity/superaerophobicity and oleophilicity/aerophilicity and improved antireflective property were successfully demonstrated on the nanosecond laser-structured titanium surfaces. Titanium materials were first transformed to be superhydrophobic after nanosecond laser ablation and low-temperature annealing treatments, showing oleophilicity/aerophilicity in water. If the surfaces were prewetted with absolute ethanol and then immersed into water, the surfaces showed superoleophobicity/superaerophobicity.

Blood coagulation dynamic testing sensor based on electromagnetic vibration.

Rapid detection techniques and methods of blood coagulation have attracted wide attention in academia and the business community in the presence of the increased demands for rapid assessment (point-of-care testing) of patients from surgery, intensive care unit, and other departments. The differential equation of vibration system composed of elastic support and electromagnetic induction devices was set up using the principle of damping vibration and establishing the dynamics model; meanwhile, the harmonic response analysis and vibration fatigue coupling analysis were carried out, the analysis results were optimized, and the experimental device of the electromagnetic induction testing sensor was established. In addition, the experimental device with blood coagulation reagent was assorted to establish the standard point-of-care testing rapid blood coagulation detection curve, and to compare the testing curve with that of the imported point-of-care testing blood coagulation instrument.

Nanosecond Laser-Induced Underwater Superoleophobic and Underoil Superhydrophobic Mesh for Oil/Water Separation.

Materials with special wettability have drawn considerable attention especially in the practical application for the separation and recovery of the oily wastewater, whereas there still remain challenges of the high-cost materials, significant time, and complicated production equipment. Here, a simple method to fabricate the underwater superoleophobic and underoil superhydrophobic brass mesh via the nanosecond laser ablation is reported for the first time, which provided the micro-/nanoscale hierarchical structures. This mesh is superhydrophilic and superoleophilic in air but superoleophobic under water and superhydrophobic under oil.

Electromagnetic induction sensor for dynamic testing of coagulation process.

With the increasing demand for coagulation POCT for patients in the surgery department or the ICU, rapid coagulation testing techniques and methods have drawn widespread attention from scholars and businessmen. This paper proposes the use of electromagnetic induction sensor probe for detection of dynamic process causing changes in the blood viscosity and density before and after coagulation based on the damped vibration principle, in order to evaluate the coagulation status. Utilizing the dynamic principle, the differential equation of vibration system comprising elastic support and electromagnetic induction device is established through sensor dynamic modeling.

Direct Adsorption of Anti-CD34 Antibodies on the Nano-Porous Stent Surface to Enhance Endothelialization.

Background: In-stent restenosis following the insertion of conventional drug-eluting stent has become an extremely serious problem due to coating techniques, with polymer matrices used to bind biological ingredients to the stent surface. However, several studies have indicated that new pro-healing technique could prevent stent thrombosis that can be caused by conventional drug-eluting stents.

Methods: A novel method of attaching anti-CD34 antibodies directly on the porous surface of a 316L stainless steel bare metal stent was developed in this study, which achieved both high stability of attached anti-CD34 antibodies on the metal stent surface and high antibody activity for stem cell capture.

The role of noggin in regulation of high glucose-induced apoptosis and insulin secretion in INS-1 rat beta cells.

Objectives: The purpose of this study was to investigate the effects of Noggin on high glucose-induced apoptosis and insulin secretion in pancreatic beta cells.

Materials And Methods: Different concentrations of glucose were used to examine their effects on INS-1 rat beta cells in vitro. When specific siRNA targeting Noggin and recombinant Noggin were added, apoptosis and insulin secretion were measured, respectively to determine their effects in INS-1 cells.

MicroRNA-577 inhibits gastric cancer growth by targeting E2F transcription factor 3.

The incidence and mortality rates of gastric cancer are one of the highest of all types of cancers. Emerging evidence has demonstrated that altered expression of micro (mi)RNAs may be implicated in the tumorigenesis of numerous types of cancer. Therefore, miRNAs may have potential as important tools in cancer diagnostics and therapeutics.

Accelerated endothelialization with a polymer-free sirolimus-eluting antibody-coated stent.

Drug-eluting stents have shown an impressive reduction of in-stent restenosis for many years. However, stent thrombosis due to incomplete/late endothelialization has raised major safety concerns. To overcome these problems, we developed for the first time a polymer-free sirolimus-eluting antibody-coated stent (PFSEACS) by combining polymer free and endothelial progenitor cell-capture pro-healing approaches.

The union of anti-CD34 antibody can improve the performance of drug-eluting stents.

Objectives: The authors investigate whether the combination of anti-CD34 antibody with DES is win-win cooperation.

Background: DES may reduce the risk of restenosis compared to bare-metal stents (BMS), but they were found to inhibit the healing process of intima.

Methods: Fifteen BMS, 17 DES, and 16 combined anti-CD34 antibody and DES were randomly implanted in the coronary arteries of 22 minipigs.

[Monitoring early toxicity of heavy metals including Hg using a HSE-SEAP reporter gene].

Objective: To develop a cellular assay based on heat shock signal pathway and secreted alkaline phosphatase (SEAP) reporter gene for investigating/predicting the early toxicity of heavy metals on HeLa cells in Chinese traditional medicine (TCM).

Method: The pHSE-SEAP plasmid was transfected into HeLa cells to build a HSE-SEAP-HeLa cell model. For validation of the model, the transfected cells were treated by either heating at 42 degrees C for 1 h or incubated with 5 mol x L(-1) CdCl2 for 4 h.

Metal ions induced heat shock protein response by elevating superoxide anion level in HeLa cells transformed by HSE-SEAP reporter gene.

The aim of this work is to define the relationship between heat shock protein (HSP) and reactive oxygen species (ROS) in the cells exposed to different concentrations of metal ions, and to evaluate a new method for tracing the dynamic levels of cellular reactive oxygen species using a HSE-SEAP reporter gene. The expression of heat shock protein was measured using a secreted alkaline phosphatase (SEAP) reporter gene transformed into HeLa cell strain, the levels of superoxide anion (O(2)(-)) and hydrogen peroxide (H(2)O(2)) were determined by NBT reduction assay and DCFH staining flow cytometry (FCM), respectively. The experimental results demonstrated that the expression of heat shock protein induced by metal ions was linearly related to the cellular superoxide anion level before cytotoxic effects were observed, but not related to the cellular hydrogen peroxide level.