Switching alkaline hydrogen oxidation reaction pathway via microenvironment modulation of Ru catalysts.

Ruthenium (Ru) has emerged as a promising catalyst for alkaline hydrogen oxidation reaction (HOR). Nevertheless, its catalytic performance still remains substantially inferior to the requirements of practical applications. Strategic modulation of the Ru micro-environment offers significant potential for optimizing its intrinsic catalytic activity.

A replicative recombinant HPV16 E7 expression virus upregulates CD36 in C33A cells.

Objective: In past decades, the role of high-risk HPV (HR-HPV) infection in cancer pathogenesis has been extensively studied. The viral E7 protein expressed in pre-malignant cells has been identified as an ideal target for immunological intervention. However, the cultivation of HPV remains a significant challenge, as well as the lack of methods for expressing the HPV E7 protein and generating replication-competent recombinant viral particles, which posed a major obstacle to further exploration of the function and carcinogenic mechanisms of the E7 oncoprotein.

Quantifying the Fast Dynamics of HClO in Living Cells by a Fluorescence Probe Capable of Responding to Oxidation and Reduction Events within the Time Scale of Milliseconds.

The biological roles of reactive oxygen species (ROS) depend highly on their dynamics. However, it has been challenging for measuring the dynamics of ROS in cells. In this study, we address a core challenge in developing fluorescence probes for monitoring ROS dynamics by designing a redox couple that can respond rapidly to both oxidation and reduction events.

Theoretical Insights into the Excited State Decays of a Donor-Acceptor Dyad: Is the Twisted and Rehybridized Intramolecular Charge-Transfer State Involved?

The twisted intramolecular charge transfer has been proposed for a number of years and widely accepted to explain the excited-state dynamics of organic molecules. Recently, a new state termed as "twisted and rehybridized intramolecular charge transfer" has been proposed to explain the excited-state dynamics of an aniline-triazine electron donor-acceptor dyad with an alkyne spacer based on ultrafast time-resolved spectroscopy. However, the change of the geometries along the excited-state decay pathway remains unknown.

Redox-Responsive Fluorescent Probes with Different Design Strategies.

In an aerobic organism, reactive oxygen species (ROS) are an inevitable metabolic byproduct. Endogenously produced ROS have a significant role in physiological processes, but excess ROS can cause oxidative stress and can damage tissue. Cells possess elaborate mechanisms to regulate their internal redox status.

Switching of the triplet excited state of rhodamine/naphthaleneimide dyads: an experimental and theoretical study.

Rhodamine-bromonaphthaleneimide (RB-NI) and rhodamine-bromonaphthalenediimide (RB-NDI) dyads were prepared for switching of the triplet excited states. Bromo-NI or bromo-NDI parts in the dyads are the spin converters, i.e.

Switching of the triplet excited state of rhodamine-C60 dyads.

Acid-switching of the triplet excited state in rhodamine-C60 dyads was achieved. The rhodamine moiety acts as an acid-activated visible light-harvesting antenna and C60 as the singlet energy acceptor and the spin converter, and production of the triplet state was enhanced in the presence of acid.

Selenium as a versatile center in fluorescence probe for the redox cycle between HClO oxidative stress and H2S repair.

Selenium is a biologically important trace element and acts as an active center of glutathione peroxidase (GPx). GPx is the important antioxidant enzyme to protect organisms from oxidative damage via catalyzing the reaction between ROS and glutathione (GSH). Mimicking the oxidation-reduction cycles of the versatile selenium core in GPx, we can develop fluorescence probes to detect oxidation and reduction events in living systems.

Experimental and theoretical study on the sensing mechanism of a fluorescence probe for hypochloric acid: a Se···N nonbonding interaction modulated twisting process.

In this article, the sensing mechanism of a fluorescence probe for hypochloric acid, NI-Se, has been investigated using experimental and theoretical methods. Based on the results of the steady-state and time-resolved emission spectra of NI-Se and its oxidized form NI-SeO, we suggested that there was twist internal charge transfer (TICT) state with faint fluorescence in NI-Se. Subsequently, the ground and excited state minimum geometries of NI-Se and NI-SeO were optimized with DFT/TD-DFT methods.

Ratiometric fluorescence imaging of cellular hypochlorous acid based on heptamethine cyanine dyes.

A series of ratiometric probes based on heptamethine cyanine dyes for detecting hypochlorous acid have been developed. Here we present the synthesis, characterization and fluorescence properties of these probes. And it turns out that the probes are highly sensitive and selective toward hypochlorous acid.

A reversible fluorescent probe for detecting hypochloric acid in living cells and animals: utilizing a novel strategy for effectively modulating the fluorescence of selenide and selenoxide.

Based on a novel strategy for modulating the fluorescence of selenide and selenoxide, we have designed and developed a reversible fluorescent probe for hypochloric acid. And the synthesis, characterization, fluorescence properties, as well as the biological applications in living cells and animals, have all been described.

A reversible fluorescence probe based on Se-BODIPY for the redox cycle between HClO oxidative stress and H2S repair in living cells.

We have developed a new reversible fluorescence probe MPhSe-BOD for the redox cycle process between hypochlorous acid and hydrogen sulfide in solution and in living cells. Confocal microscopy imaging using RAW264.7 cell lines shows that the probe has good cell membrane permeability, and can monitor intracellular HClO/H(2)S redox cycles continuously.

A fluorescent probe for rapid detection of thiols and imaging of thiols reducing repair and H2O2 oxidative stress cycles in living cells.

A diselenide containing fluorescent probe based on a fluorescein scaffold for thiols was developed. The fluorescent probe exhibited rapid response, high selectivity and reversibility. Confocal fluorescence microscopy was used to visualize the redox changes mediated by thiols and reactive oxygen species in living HeLa cells.