Publications by authors named "Zhangbin Bao"
We report the development of a self-renewable tag (srTAG) for protein fluorescence imaging. srTAG leverages the "on-protein" fluorophore equilibrium between the fluorescent zwitterion and non-fluorescent spirocyclic form and the reversible fluorescence labeling to enable self-recovery of fluorescence after photobleaching. This small-sized srTAG allows 2-6 times longer imaging duration compared to other commonly used self-labeling tags and is compatible with fluorophores with different spectral properties.
View Article and Find Full Text PDFTerrestrial organisms developed circadian rhythms for adaptation to Earth's quasi-24-h rotation. Achieving precise rhythms requires diurnal oscillation of fundamental biological processes, such as rhythmic shifts in the cellular translational landscape; however, regulatory mechanisms underlying rhythmic translation remain elusive. Here, we identified mammalian ATXN2 and ATXN2L as cooperating master regulators of rhythmic translation, through oscillating phase separation in the suprachiasmatic nucleus along circadian cycles.
View Article and Find Full Text PDFArticle Synopsis
- Retroelements are key players in genome evolution and have potential uses as gene-editing tools; this study focuses on the eukaryotic R2 retrotransposon and its interactions with DNA and RNA.
- The researchers used cryo-EM imaging along with biochemical and sequencing methods to identify two important DNA regions, Drr and Dcr, necessary for the retrotransposon's ability to recognize and cleave DNA.
- They found that regulatory RNAs play a crucial role by influencing the timing of DNA cleavage and reverse transcription, leading to a better understanding of retrotransposon mechanics and possible applications in genetic reprogramming.