Circ-USP9X accelerates deep vein thrombosis after fracture by acting as a miR-148b-3p sponge and upregulates SRC kinase signaling inhibitor 1.

Objectives: This study aims to elucidate the role of circUSP9X (Circular RNA Ubiquitin Specific Peptidase 9 X-Linked) in the development of venous thrombosis in the lower extremities.

Methods: An animal model of Deep Vein Thrombosis (DVT) and a hypoxic model of Human Umbilical Vein Endothelial Cells (HUVECs) treated with Cobalt (II) Chloride (CoCl) were developed. The expression levels of circUSP9X, microRNA-148b-3p (miR-148b-3p), and SRC Kinase Signaling Inhibitor 1 (SRCIN1) were quantified using quantitative reverse transcription Polymerase Chain Reaction and Western blot analysis.

Microfracture technique combined with mesenchymal stem cells inducer represses miR-708-5p to target special at-rich sequence-binding protein 2 to drive cartilage repair and regeneration in rabbit knee osteoarthritis.

Knee osteoarthritis (KOA) is a degenerative joint illness which leads to knee pain and functional limitation. In this study, we combined microfracture surgery with kartogenin (KGN), a small bioactive molecule used to promote the differentiation of mesenchymal stem cells (MSCs), and explored its impact on cartilage repair and possible latent mechanisms of action. The research offers a brand-new idea for the clinical cure of KOA.

Design and localization algorithm of flap guide plate for jaw defect based on digital 3D printing.

The anterolateral thigh flap is often used to repair the wound tissue defect. Because it is difficult to carry out the perforating vessels before and after the operation, it is considered to use digital design combined with 3D printing technology to prepare the digital three-dimensional guide plate, and at the same time, the guide plate positioning algorithm is designed according to the error caused by the different placement of the guide plate at the site to be transplanted. First of all, select the patients with jaw defect, make the patient's jaw model, obtain the corresponding plaster working model through 3D scanning, obtain STL data, design the guide plate in combination with Rihnoceros and other software, and print the flap guide plate corresponding to jaw defect with the help of metal powder using 3D printer.

Feasibility of polyethylene composites reinforced by distillers dried fibers with solubles (DDFS) after different generations of ethanol fermentation.

In order to effectively evaluate the distillers dried fibers with solubles (DDFS) obtained from biorefinery processes, sorghum distiller (SD), cassava distiller (CD) and corn cob distiller (CCD) residuals from different generation bioethanol plants were used as the reinforcing phase for polyethylene composites. The mechanical performances and the physical properties of the polyethylene/DDFS composites were evaluated. The results showed that the CCD reinforced specimen offered the best mechanical performances, with a flexural strength of 21.

Effect of dilute alkaline pretreatment on the conversion of different parts of corn stalk to fermentable sugars and its application in acetone-butanol-ethanol fermentation.

To investigate the effect of dilute alkaline pretreatment on different parts of biomass, corn stalk was separated into flower, leaf, cob, husk and stem, which were treated by NaOH in range of temperature and chemical loading. The NaOH-pretreated solid was then enzymatic hydrolysis and used as the substrate for batch acetone-butanol-ethanol (ABE) fermentation. The results demonstrated the five parts of corn stalk could be used as potential feedstock separately, with vivid performances in solvents production.

Effect of acid pretreatment on different parts of corn stalk for second generation ethanol production.

In this study, the effects of different parts of corn stalk, including stem, leaf, flower, cob and husk on second generation ethanol production were evaluated. FTIR, XRD and SEM were performed to investigate the effect of dilute acid pretreatment. The bagasse obtained after pretreatment were further hydrolyzed by cellulase and used as the substrate for ethanol fermentation.