Compared with the well-established functions of sympathetic innervation, the role of sensory afferents in adipose tissues remains less understood. Recent work has revealed the anatomical and physiological significance of adipose sensory innervation; however, its molecular underpinning remains unclear. Here, using organ-targeted single-cell RNA sequencing, we identified the mechanoreceptor PIEZO2 as one of the most prevalent receptors in fat-innervating dorsal root ganglia (DRG) neurons.
View Article and Find Full Text PDFThe degradation of electrical insulation is mainly attributed to local defects. Although incorporating organic small molecules into dielectric polymers promotes the insulation strength, accurate suppression of defect development is a long-term and formidable challenge. Here we utilize the adaptive charge capture methodology to achieve precise defect suppression, leading to a 123% increase in the initiation voltage of electrical trees in anthrone/cross-linked polyethylene, significantly outperforming existing dielectric polymers and polymer composites.
View Article and Find Full Text PDFJ Colloid Interface Sci
June 2025
How to establish a fast, remarkable, and different external stimulus response mechanism between similar "patterns" and "backgrounds" is a key issue in the design and preparation of invisible printed fabric based on photonic crystals. SiO and P(St-MAA) were used to construct photonic crystals, which were used as "patterns" and "backgrounds" to prepare patterned invisible printed fabric. It also reveals the relationship between the size of the structural unit and the reflection wavelength of the photonic crystals and adjusts the structure of the two parts of the photonic crystals by controlling the particle size of the microspheres so that the "pattern" can achieve the effect of "invisible in the air, visible in the water.
View Article and Find Full Text PDFThis study investigates the effect of principal leadership on teacher commitment and subjective well-being in the sport education context of China with subjective well-being as a mediator and teacher altruism as a moderator. SMART-PLS is used for data analysis and the result shows that principal leadership plays a positive role in teacher commitment and wellbeing. In addition, the mediating roles of teacher's subjective well-being as well as the moderating role of teacher altruism are confirmed.
View Article and Find Full Text PDFGlioblastoma (GBM) exhibits significant intratumor heterogeneity (ITH), indicating the presence of tumor cells with diverse growth rates. Here, we aimed to identify fast-growing cells in GBM and elucidate the underlying mechanisms. Precisely targeting these cells could offer an improved treatment option.
View Article and Find Full Text PDFOvarian cancer (OV) remains the deadliest gynecological malignancy, with non-coding RNA-mediated transcriptomic deregulation significantly influencing its prognosis and heterogeneous progression. In this study, we prioritized miRNA-mediated gene expression profiles by identifying key negative correlations between miRNA-mRNA pairs. We developed a machine learning-based non-coding index (NCI), incorporating a four-gene signature (GAS1, GFPT2, ZFHX4, and KCNA1) to predict patient prognosis and therapeutic response.
View Article and Find Full Text PDFAngew Chem Int Ed Engl
March 2025
Catalytic epoxidation of readily available aldehydes with diazo compounds is an atom economical strategy to synthesize epoxides. However, the asymmetric epoxidation reaction of aldehydes and fluorinated diazo compounds is challenging, because of the severe racemic background reaction. Herein, we report the first Cu(I)-catalyzed enantioselective epoxidation reaction between aldehydes and α-trifluoromethyl diazosilanes for the synthesis of chiral trifluoromethyl epoxides.
View Article and Find Full Text PDFFat mass and obesity-associated protein (FTO) is the key demethylase that reverses the abnormally altered N6-methyladenosine (m6A) modification in eukaryotic cells under environmental pollutants exposure. Arsenic is an environmental metalloid and can cause severe symptoms in human mainly through drinking water. However, there is no specific treatment for its toxic effects due to the uncovered mechanisms.
View Article and Find Full Text PDFThe Brook rearrangement has emerged as one of the most pivotal transformations in organic chemistry, with broad applications spanning organic synthesis, drug design, and materials science. Since its discovery in the 1950s, the anion-mediated Brook rearrangement has been extensively studied, laying the groundwork for the development of numerous innovative reactions. In contrast, the radical Brook rearrangement has garnered comparatively less attention, primarily due to the challenges associated with the controlled generation of alkoxyl radicals under mild conditions.
View Article and Find Full Text PDFHanbury-Brown and Twiss (HBT) effect is the foundation for stellar intensity interferometry. However, it is a phase insensitive two-photon interference effect. Here we extend the HBT interferometer by mixing intensity-matched reference fields with the input fields before intensity correlation measurement.
View Article and Find Full Text PDFInt J Biol Macromol
January 2025
Interleukin-2 (IL-2) is a T cell growth factor that is essential for the proliferation of T cells and the generation of effector and memory cells. The antitumor activity of high-dose IL-2 therapy requires maintaining the affinity between IL-2 and IL2-Rα, which can also bring serious toxic side effects. To address this issue, we designed ZGP-Cysteamine-IL-2-K64C and (ZGP-Cysteamine)-IL-2-(K43C, K64C) based on the strategy of FAPα enzyme-activated prodrugs, and investigated their anti-tumour activity and side effects.
View Article and Find Full Text PDFCompared to the well-established functions of sympathetic innervation, the role of sensory afferents in adipose tissues remains less understood. Recent work revealed the anatomical and physiological significance of adipose sensory innervation; however, its molecular underpinning remains unclear. Here, using organ-targeted single-cell RNA sequencing, we identified the mechanoreceptor PIEZO2 as one of the most prevalent receptors in fat-innervating dorsal root ganglia (DRG) neurons.
View Article and Find Full Text PDFMedicine (Baltimore)
November 2024
By analyzing the relevant literature, we can accurately grasp the current status of diagnosis and treatment of chronic total occlusion of coronary artery, and clarify the development trend, research frontiers and hotspots of this disease. A literature search with "chronic total occlusion" as the title was performed in the Web of Science database. The title, author, abstract, keywords, institution, publication, country, reference, and other endnotes of the selected literature were exported in the form of text.
View Article and Find Full Text PDFZinc oxide nanoparticles (ZnONPs) are widely utilized across various industries, raising concerns about their potential toxicity, especially in the respiratory system. This study explores the role of autophagy, regulated by microtubule-associated protein 1A/1B-light chain 3B (LC3B), in ZnONPs-induced toxicity using both in vivo (LC3B knockout mice) and in vitro (BEAS-2B cells) models. Our findings demonstrate that LC3B-regulated autophagy mitigates ZnONPs-induced epithelial cell dysfunction and acute lung injury.
View Article and Find Full Text PDFA novel bacterial strain, designated DW002, was isolated from the sea ice of Cape Evans, McMurdo Sound, Antarctica. Cells of the strain were Gram-negative, obligate anaerobic, motile, non-flagellated, and short rod-shaped. The strain DW002 grew at 4-32 ℃ (optimum at 22-28 ℃) and thrived best at pH 7.
View Article and Find Full Text PDFObesity has emerged as a significant global health burden, exacerbated by serious side effects associated with existing anti-obesity medications. Celastrol (CLT) holds promise for weight loss but encounters challenges related to poor solubility and systemic toxicity. Here, we present chondroitin sulfate (CS)-derived micelles engineered for adipocyte-specific targeting, aiming to enhance the therapeutic potential of CLT while minimizing its systemic toxicity.
View Article and Find Full Text PDFAcylsilanes are emerging bench-stable reagents for the generation of electron-rich oxycarbenes that are difficult to access with unstable diazo compounds. Herein, we report a siloxycarbene-mediated stereoselective synthesis of silyl enol ethers through visible-light-induced intermolecular reactions between acylsilanes and α,β-unsaturated ketones. Both the solvent and low temperature are important for the success of the reaction.
View Article and Find Full Text PDFIntroduction: The aim of this study was to investigate the autoinflammatory effect and biological behaviour of simvastatin (SIM) on adamantinomatous craniopharyngioma (ACP) cells.
Methods: Craniopharyngiomas imaging, intraoperative observations, and tumour histopathology were employed to investigate the correlation between esters and craniopharyngiomas. Filipin III fluorescent probe verified the validity of SIM on the alternations of synthesized cholesterol in craniopharyngioma cells.
Leveraging AAVs' versatile tropism and labeling capacity, we expanded the scale of in vivo CRISPR screening with single-cell transcriptomic phenotyping across embryonic to adult brains and peripheral nervous systems. Through extensive tests of 86 vectors across AAV serotypes combined with a transposon system, we substantially amplified labeling efficacy and accelerated in vivo gene delivery from weeks to days. Our proof-of-principle in utero screen identified the pleiotropic effects of Foxg1, highlighting its tight regulation of distinct networks essential for cell fate specification of Layer 6 corticothalamic neurons.
View Article and Find Full Text PDFN6-methyladenosine (m6A) is the most common form of internal post-transcriptional methylation observed in eukaryotic mRNAs. The abnormally increased level of m6A within the cells can be catalyzed by specific demethylase fat mass and obesity-associated protein (FTO) and stay in a dynamic and reversible state. However, whether and how FTO regulates oxidative damage via m6A modification remain largely unclear.
View Article and Find Full Text PDFWastewater monitoring may be a valuable early surveillance tool for studying mpox virus (MPXV) circulation in China, a country with high population density and very few mpox patients. To evaluate the effectiveness of wastewater monitoring for MPXV in detecting local hidden transmission of the epidemic in the early period, the Chinese Center for Disease Control and Prevention initiated a wastewater monitoring program for MPXV in China in July 2023. To enhance the monitoring sensitivity of the program, an MPXV monitoring point was established in a gathering place of high-risk mpox population.
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