ChNLRC4, a cytoplasmic pattern recognition receptor, activates the pyroptosis signaling pathway in Mollusca.

NLR inflammasomes recognize pathogen-associated molecular patterns (PAMPs), triggering Caspase-1 activation and leading to gasdermin D (GSDMD)-mediated pyroptosis, a crucial immune response in mammals. The functional GSDME-mediated pyroptosis has been reported in invertebrates, yet the existence of an NLR-Caspase-GSDME axis mediating pyroptosis signaling cascades remains unclear. In this study, we reported an NLRC4 homolog named ChNLRC4, a pattern recognition receptor from the oyster Crassostrea hongkongensis that is able to bind to LPS and Lys-type PGN through its LRR domain.

Establishment and Characterization of Bisexually Fertile Triploid Dwarf Surf Clam Mulinia lateralis.

Triploids are widely used to rapidly achieve genetic improvements of organisms due to their fast growth and enhanced environmental adaptability. Artificially induced triploids are generally considered to be infertile owing to the obvious inhibition of gonadal development. Recently, some fertile individuals with reduced advantages have been found in triploid bivalves, which is a notable deviation from the original intention of artificially inducing triploids.

Diel transcriptional responses of coral-Symbiodiniaceae holobiont to elevated temperature.

Coral exhibits diel rhythms in behavior and gene transcription. However, the influence of elevated temperature, a key factor causing coral bleaching, on these rhythms remains poorly understood. To address this, we examined physiological, metabolic, and gene transcription oscillations in the Acropora tenuis-Cladocopium sp.

Research on In-Plane Thermal Conductivity Detection of Fuel Cell Bipolar Plates Based on Laser Thermography.

The thermal properties of bipolar plates, being key elements of polymer electrolyte membrane fuel cells, significantly affect their heat conduction and management. This study employed an innovative approach known as a heat flow loop integral method to experimentally assess the in-plane thermal conductivity of graphite bipolar plates, addressing the constraints of traditional methods that have strict demands for thermal stimulation, boundary or initial conditions, and sample size. This method employs infrared thermal imaging to gather information from the surface temperature field of the sample, which is induced by laser stimulation.

ChRIPK1 caused necroptosis signaling pathway deficiency in Crassostrea hongkongensis.

RIPK1/TAK1 are important for programmed cell death, including liver death, necroptosis and apoptosis. However, there have been few published reports on the functions of RIPK1/TAK1 in invertebrates. In this study, full-length ChRIPK1 and ChTAK1 were cloned from C.

Atomic Ru-Pt dual sites boost the mass activity and cycle life of alkaline hydrogen evolution.

The development of highly efficient and ultrastable electrocatalysts for hydrogen generation from water/real seawater faces huge challenges. Herein, porous carbon-supported amorphous RuPt nanoclusters (RuPt/PC) achieve mass activities of 42.28/10.

A Testis-Specific (Double Sex and Mab-3-Related Transcription Factor 1) Plays a Role in Spermatogenesis and Gonadal Development in the Hermaphrodite Boring Giant Clam .

The testis-specific double sex and mab-3-related transcription factor 1 () has long been recognized as a crucial player in sex determination across vertebrates, and its essential role in gonadal development and the regulation of spermatogenesis is well established. Here, we report the cloning of the key spermatogenesis-related cDNA, named , from the gonads of (), with a molecular weight of 41.93 kDa and an isoelectric point of 7.

Aquaculture Performance and Genetic Diversity of a New [( ♀ × . ♂) ♂ × ♀] Variety of the Oyster "South China No. 1" in Beibu Gulf, China.

is an economically important bivalve found in various parts of the South China Sea. A new interspecific backcross ([( ♀ × . ♂) ♂ × ♀]) variety was bred by the South China Sea Institute of Oceanology which named "South China No.

Resistance of an intertidal oyster(Saccostrea mordax)to marine heatwaves and the implication for reef building.

Marine heatwaves (MHWs) have a significant impact on intertidal bivalves and the ecosystems they sustain, causing the destruction of organisms' original habitats. Saccostrea mordax mainly inhabits the intertidal zone around the equator, exhibiting potential tolerance to high temperatures and maybe a species suitable for habitat restoration. However, an understanding about the tolerance mechanism of S.

Identification and involvement of DAX1 gene in spermatogenesis of boring giant clam Tridacna crocea.

DAX1 (dosage-sensitive sex reversal, adrenal hypoplasia congenital critical region on X chromosome gene 1), a key sex determinant in various species, plays a vital role in gonad differentiation and development and controls spermatogenesis. However, the identity and function of DAX1 are still unclear in bivalves. In the present study, we identified a DAX1 (designed as Tc-DAX1) gene from the boring giant clam Tridacna crocea, a tropical marine bivalve.

Chromosome-level genome assembly and annotation of rare and endangered tropical bivalve, Tridacna crocea.

Tridacna crocea is an ecologically important marine bivalve inhabiting tropical coral reef waters. High quality and available genomic resources will help us understand the population structure and genetic diversity of giant clams. This study reports a high-quality chromosome-scale T.

A novel Fas ligand plays an important role in cell apoptosis of : molecular cloning, expression profiles and functional identification of FasL.

Background: Apoptosis regulates normal development, homeostasis, immune tolerance and response to environmental stress by eliminating unwanted or diseased cells, and plays a key role in non-specific immunity of invertebrates. The exogenous pathway mediated by death receptors and death ligands is a very important pathway for cell apoptosis. Death ligands are mainly members of the tumour necrosis factor (TNF) family, of which FasL is an important member.

Comparison of biochemical composition, nutritional quality, and metals concentrations between males and females of three different Crassostrea sp.

Gametogenesis can significantly affect the biochemical composition of oysters, but little research on the difference between sexes. Therefore, we conducted the first in-depth study on the composition differences between males and females of three different Crassostrea sp..

Adaptive Bird-like Genome Miniaturization During the Evolution of Scallop Swimming Lifestyle.

Genome miniaturization drives key evolutionary innovations of adaptive traits in vertebrates, such as the flight evolution of birds. However, whether similar evolutionary processes exist in invertebrates remains poorly understood. Derived from the second-largest animal phylum, scallops are a special group of bivalve molluscs and acquire the evolutionary novelty of the swimming lifestyle, providing excellent models for investigating the coordinated genome and lifestyle evolution.

Serum amyloid protein (SAA) as a healthy marker for immune function in Tridacna crocea.

Serum amyloid protein (SAA) is known as an acute reactive protein of innate immunity in mammals. However, in invertebrates, the role of SAA in innate immunity is still unclear. In this study, a full-length cDNA of the SAA gene (named TcSAA) was cloned from Tridacna crocea, mollusca.

Comparative Genomics Reveals Evolutionary Drivers of Sessile Life and Left-right Shell Asymmetry in Bivalves.

Bivalves are species-rich mollusks with prominent protective roles in coastal ecosystems. Across these ancient lineages, colony-founding larvae anchor themselves either by byssus production or by cemented attachment. The latter mode of sessile life is strongly molded by left-right shell asymmetry during larval development of Ostreoida oysters such as Crassostrea hongkongensis.

Assessment of the juvenile vulnerability of symbiont-bearing giant clams to ocean acidification.

Ocean acidification (OA) severely affects marine bivalves, especially their calcification processes. However, very little is known about the fate of symbiont-bearing giant clams in the acidified oceans, which hinders our ability to develop strategies to protect this ecologically and economically important group in coral reef ecosystems. Here, we explored the integrated juvenile responses of fluted giant clam Tridacna squamosa (Lamarck, 1819) to acidified seawater at different levels of biological organization.

MDM2 is involved in the regulation of p53 expression in the immune response of oyster Crassostrea hongkongensis.

MDM2 (mouse double-minute) and p53 form a negative feedback loop and play a prominent role in preventing the induction of uncontrolled apoptosis. To better understand their potential roles in oyster Crassostrea hongkongensis, MDM2 and p53 homologs were first isolated and cloned in C. hongkongensis (named ChMDM2 and Chp53), and their mRNA expression patterns in tissues and developmental stages were analyzed.

Transcriptome analysis reveals acclimation responses of pearl oysters to marine heatwaves.

Marine heatwaves (MHWs) are weather-timescale extreme events in the oceans and can have devastating effects on marine bivalves and ecosystems they support, with considerable socio-economic consequences. Yet, the extent to which marine bivalves have the capacity to acclimate and adapt to MHWs remains unknown. Understanding molecular responses to MHWs is imperative to develop strategies for conservation of ecologically and economically important marine organisms.

Impacts of marine heatwaves on pearl oysters are alleviated following repeated exposure.

Marine heatwaves (MHWs) have occurred with increasing duration, frequency and intensity in the past decade in the South China Sea, posing serious threats to marine ecosystems and fisheries. However, the impact of MHWs on marine bivalves - one of the most ecologically and economically important fauna in coastal ecosystems - remains largely unknown. Here, we investigated physiological responses of the pearl oyster, Pinctada maxima inhabiting a newly identified climate change hotspot (Beibu Gulf, South China Sea) to short-lasting and repeatedly-occurring MHWs scenarios.

Repeated exposure to simulated marine heatwaves enhances the thermal tolerance in pearl oysters.

In an era of unprecedented climate change, marine heatwaves (MHWs) are projected to increase in frequency, intensity, and duration, severely affecting marine organisms and fisheries and causing profound ecological and socioeconomic impacts. However, very little is known about effects of MHWs on ecologically and economically important bivalve species. Here, we investigate how pearl oysters, Pinctada maxima (Jameson), respond to MHWs under various simulated scenarios.

Sequencing and analysis of the complete mitochondrial genome of .

In this study, the complete mitochondrial genome of was reported. The whole mitochondrial genome was 21,565bp in length with a typical mitochondrial genomic structure including 13 protein-coding genes, 23 transfer RNA genes, 2 ribosomal RNA genes and 1 control region (D-loop). Mitogenome base composition was biased toward A + T content, at 60.

Tetraploid induction of and by inhibiting the polar body 1 release in diploid fertilized eggs.

The production of an all-triploid population by mating tetraploid males with diploid females is the best and most fundamental method for the large-scale production of triploid oysters. Obtaining a stable tetraploid population is essential for guaranteed production in industrialized triploid cultivation. and are important oyster breeding species in southern China, and have great economic value.