[Determination of HPLC fingerprint of Qingfengtong capsule].

Objective: To establish a fingerprint analysis method of Qingfengtong capsule by HPLC.

Method: The samples were extracted with 70% ethanol in an ultrasonic bath. The extracts were analyzed at 35 degrees C on a Diamonsil C18 column (4.

Plasmid-based vaccination with candidate anthrax vaccine antigens induces durable type 1 and type 2 T-helper immune responses.

The current anthrax vaccine imparts protective immunity by generating a humoral immune response against a single antigen, the PA exotoxin subunit. While this response neutralizes the two anthrax exotoxins and protects the recipient from toxin-related mortality, the recipient is not protected from spore germination, infection, and/or bacteremia. Moreover, protective immunity against PA must be generated via a lengthy injection schedule and maintained by a yearly booster.

[Determination of chyodeoxycholic acid in Bile Arisaema by HPLC-ELSD].

Objective: To develop an HPLC method for the determination of hyodeoxycholic acid in Bile Arisaema.

Method: The methanol extracts were separated on a Diamonsil ODS column eluted at 35 degrees C with a mixture of methanol-water-glacial acetic acid (75:25:0.01) at flow-rate 1.

Rectification of age-associated deficiency in cytotoxic T cell response to influenza A virus by immunization with immune complexes.

Decline in cellular immunity in aging compromises protection against infectious diseases and leads to the increased susceptibility of the elderly to infection. In particular, Ag-specific cytotoxic T lymphocyte (CTL) response against virus is markedly reduced in an aged immune system. It is of great importance to explore novel strategy in eliciting effective antiviral CTL activity in the elderly.

Control of mucosal virus infection by influenza nucleoprotein-specific CD8+ cytotoxic T lymphocytes.

Background: MHC class I-restricted CD8+ cytotoxic T lymphocytes (CTL) are thought to play a major role in clearing virus and promoting recovery from influenza infection and disease. This has been demonstrated for clearance of influenza virus from the lungs of infected mice. However, human influenza infection is primarily a respiratory mucosal infection involving the nasopharynx and tracheobronchial tree.

[Hepatic safety of itraconazole intravenous solution in treatment of invasive fungal infection].

Objective: To investigate the hepatic safety of itraconazole intravenous solution in the treatment of invasive fungal infection.

Methods: Forty-nine patients with invasive fungal infection, such as pneumonia, meningitis, endocarditis, and blood stream infection, caused by Aspergillus spp. Cryptococcus neoformans, Candida spp.

[Study on the cholelitholytic effect and safety of the preparation of Chinese medicine compound].

Objective: To study the cholelitholytic effect and safety of the preparation of Chinese medicine compound.

Method: Experiments were performed to study the efficacy of gallstones dissolution of the preparation of Chinese medicine compound in vitro and in vivo. The toxicity was studied by pathological and blood biochemical changes.

[Evaluation of green tangerine peel processed with vinegar using HPLC fingerprint technique].

Objective: To develop an HPLC method for determination of fingerprints and evaluate green tangerine peel (GTP) processed with vinegar.

Method: The samples were extracted with 50% ethanol using ultrasonic bath and analyzed on a Chromasil C18 column eluted with methanol and water containing 0.2% acetic acid in a linear gradient program.

[Qualitative and quantitative methods of betulonic acid in fruits of Liquidambar formosana].

Objective: To develop the identification and assay methods for betulonic acid in fruits of Liquidambar formosana.

Method: TLC method was used for identification with silica gel G plate and petroleum ether(60-90 degrees C)-acetone (17:3) as a developing solvent. Betulonic acid in ethanol extract was separated on the ODS column with methanol-water-glacial acetic acid (87:13:0.

[Determination of flavonoids in buds of Herba Artemisiae Scopariae by HPLC].

Objective: To develop a quantitative method for the determination of four flavonoids in buds of Herba Artemisiae Scopariae.

Method: The sample was extracted by ultrasonic with ethyl acetate for 30 minutes and separated on kromasil ODS column with mobile phase of acetonitrile-tetrahydrofuran-1% acetic acid solution (2.5:21.

[Study on traditional Chinese pharmaceutical processing of radix Pinellia pedatisecta].

Objective: To study the effects of concentration, volume and temperature of aluminium water on the change of chromatoghraphic data of processed Rhizoina Arisaematis.

Method: Chromatographic fingerprints of processed Radix Pinellia pedatisecta were determined by HPLC, and multivariate analysis was made for data processing.

Result And Conclusion: No change on the whole chromatoghraphic data was observed at different levels of concentrition and volume of aluminium water, while there was a great change at different levels of temperature.

[Study on extraction conditions for minute powder of processed rhizome of Corydalis with vinegar].

Objective: To examine the factors in extracting the minute powder of processed rhizome of Corydalis with vinegar for its application.

Method: The one-way variation analysis was used for experimental design. Tetrahydropalmatine was determined with HPLC method.

[Study on applying chromatographic fingerprints to establishing quality criterion of processed tuber of Pinellia pedatisecta].

Objective: To explore the utility of principal component analysis (PCA) in conjunction with the window preprocessing scheme on chromatographic data for quality control.

Method: Chromatographic fingerprints of processed tuber of Pinellia pedatisecta were determined by HPLC, and the Window Preprocessing and PCA were used for data processing.

Result: The quantitative differences among different growing areas and different process batches were found with this method.

Basaloid squamous cell carcinoma of the esophagus with or without adenoid cystic features.

Context: Basaloid squamous cell carcinoma (BSCC) of the esophagus is a rare malignant tumor that morphologically could bear some resemblance to adenoid cystic carcinoma (ACC) originating from salivary glands.

Objective: The purpose of this study is to describe the histologic, immunohistochemical, and ultrastructural findings of BSCCs of the esophagus, with an emphasis on comparing tumors with or without adenoid cystic features.

Design: We reviewed 239 cases of primary esophageal carcinoma and detected 12 cases (5%) of BSCC.

Major outbreak of toxic shock-like syndrome caused by Streptococcus mitis.

Severe illness caused by viridans streptococci rarely occurs in immunocompetent hosts. Between December 1990 and May 1991, thousands of patients in the YangZi River Delta area of Jiangsu Province, China, suffered from scarlet fever-like pharyngitis. Fewer cases occurred in subsequent years with the same seasonality.

[Qualitative and quantitative methods of chlorogenic acid in commercial Herba Artemisiae Scopariae].

Objective: To develop the identification and assay for chlorogenic acid in commercial Herba Artemisiae Scopariae.

Method: TLC method was used for identification with silica gel G plate and butyl acetate-formic acid-water (7:2.5:2.

[Determination of cirsilineol in Herba Artemisiae scopariae collected in autumn by HPLC].

Objective: To establish a quantitative method for determination of cirsilineol in Herba Artemisiae Scopariae collected in Autumn.

Method: Sample was extracted with methanol, and cleaned up with polyamide column. ODS column was used with methanol-acetonitrile-0.

[Assay for simultaneous detection of HIV p24 antigen and anti-HIV antibody].

Objective: To develop a rapid assay for simultaneous detection of HIV p24 antigen (Ag) and anti-HIV antibody (Ab).

Methods: HIV-1 gp41 antigen and HIV-2 gp36 antigen were expressed by recombinant baculovirus insect system and purified by immunochromatography. p24 monoclonal antibody (mAb) was obtained from p24 hybridoma cell line.

Cationic liposome-mediated enhanced generation of human HLA-restricted RSV-specific CD8+ CTL+.

Generation of human CD8+ cytotoxic T-lymphocyte (CTL) activity against respiratory syncytial virus (RSV) using peripheral blood leukocytes (PBL) in vitro is inefficient. Lipofectamine, a polycationic liposome, previously shown to enhance the transfection efficiency of DNA in cells, was evaluated for enhancing RSV CTL activity. Stimulator cells were prepared by infecting human PBL with RSV with or without Lipofectamine for 3 hr and then transferred to responder cells.

Immunoglobulin A-deficient mice exhibit altered T helper 1-type immune responses but retain mucosal immunity to influenza virus.

We have previously demonstrated that immunoglobulin A (IgA)(-/-) knockout (KO) mice exhibit levels of susceptibility to influenza virus infection that are similar to those of their normal IgA(+/+) littermates. To understand the mechanism of this apparent mucosal immunity without IgA, immunoglobulin isotype and T helper 1 (Th1)-type [interferon-gamma (IFN-gamma)] and Th2-type [interleukin (IL)-4, IL-5)] cytokine responses to influenza vaccine were evaluated. Intranasal immunization with influenza virus subunit vaccine plus cholera toxin/cholera toxin B subunit (CT/CTB) induced significant influenza virus-specific immunoglobulin G (IgG) antibody in the serum and nasal passages of both IgA(-/-) and IgA(+/+) mice, while IgA antibodies were induced only in IgA(+/+) mice.

An aged mouse model for RSV infection and diminished CD8(+) CTL responses.

Recent studies indicate that respiratory syncytial virus (RSV), like influenza, causes significant morbidity and mortality among elderly persons. There are currently no animal models to study the effects of aging on RSV disease and immunity. This manuscript provides an initial description of such a model.

Mice deficient for the wild-type p53-induced phosphatase gene (Wip1) exhibit defects in reproductive organs, immune function, and cell cycle control.

The Wip1 gene is a serine/threonine phosphatase that is induced in a p53-dependent manner by DNA-damaging agents. We show here that Wip1 message is expressed in moderate levels in all organs, but is present at very high levels in the testes, particularly in the postmeiotic round spermatid compartment of the seminiferous tubules. We have confirmed that Wip1 mRNA is induced by ionizing radiation in mouse tissues in a p53-dependent manner.