Coherence-Based Graph Convolution Network to Assess Brain Reorganization in Spinal Cord Injury Patients.

Motor imagery (MI) engages a broad network of brain regions to imagine a specific action. Investigating the mechanism of brain network reorganization during MI after spinal cord injury (SCI) is crucial because it reflects overall brain activity. Using electroencephalogram (EEG) data from SCI patients, we conducted EEG-based coherence analysis to examine different brain network reorganizations across different frequency bands, from resting to MI.

In vitro and in vivo assessment of diosmetin-loaded lactoferrin-modified liposomes for brain delivery in intracerebral hemorrhage therapy.

Intracerebral hemorrhage (ICH) is a serious cerebrovascular disease with high morbidity, mortality, and disability rates, largely due to neuroinflammation. Diosmetin, a natural flavonoid, has known neuroprotective effects in cerebral ischemia/reperfusion models but has been less studied in ICH. Our previous study developed diosmetin-loaded lactoferrin-modified long-circulating liposomes (Lf-Dios-Lcl), which penetrate the BBB and improve diosmetin bioavailability and brain distribution.

Papillary thyroid carcinoma and strumal carcinoid transformations of a mature cystic teratoma with NRAS codon 61 mutation: A case report.

Mature cystic teratoma(MCT) is the most prevalent neoplasm derived from germ cells in females; its progression to malignancy is infrequent. The coexistence of papillary thyroid carcinoma(PTC) and strumal carcinoid derived from MCT is exceptionally rare. To the best of our knowledge, only six cases of this condition have been reported to date, however, the molecular information and immune cell infiltration for the mixture has never been studied.

Whole-genome resequencing reveals the population structure and domestication processes of endemic endangered goose breeds (Anser cygnoides).

In recent years, the dwindling population of these endangered geese has hindered our understanding of their phenotypic variations and the genes associated with important traits. To investigate the population structure and genetic diversity of this breed, the whole-genome data of 90 individuals from a conservation farm were obtained using the Illumina 6000 paired-end platform. The research results indicate that each locally endangered goose variety has formed a monophyletic population.

Analgesic effect of ropivacaine combined with methylene blue in fascia Iliaca block for patients undergoing hip arthroplasty.

Background: The duration of a single fascia iliaca compartment block (FICB) with ropivacaine is limited. This study investigated whether methylene blue as an adjuvant anesthetic in FICB can enhance the postoperative analgesic effect following total hip arthroplasty (THA).

Methods: Patients who planned to undergo THA were recruited for this randomized clinical trial from June 2023 to February 2024.

Clinical Analyses of 4 Cases of Microsporidial Keratoconjunctivitis.

Objective: To report four cases of microsporidial keratoconjunctivitis (MKC) from The Affiliated Eye Hospital of Nanjing Medical University (from May 2023 to October 2024) and to aid ophthalmologists in diagnosing and treating MKC, as MKC has been increasingly reported in Asian healthy individuals but not much in Mainland China.

Methods: Four patients with MKC were studied. Demographic information, symptoms, and clinical data were collected.

The active ingredient β-sitosterol in the anti-inflammatory agents alleviates perianal inflammation in rats by inhibiting the expression of Srebf2, activating the PPAR signaling pathway, and altering the composition of gut microbiota.

Background: Anti-inflammatory herbal formulations are common in traditional Chinese medicine for clearing heat and detoxifying; however, the specific active components and their mechanisms remain unclear.

Objective: This study investigates the role of Sitosterol in alleviating perianal inflammation and its underlying mechanisms.

Methods: Sitosterol was identified as a key active ingredient through the TCMSP database.

Recent advances in bioresponsive macrocyclic gadolinium(III) complexes for MR imaging and therapy.

Magnetic resonance (MR) imaging is a non-invasive clinical diagnostic modality that provides anatomical and physiological information with sub-millimetre spatial resolution at the organ and tissue levels. It utilizes the relaxation times ( and ) of protons in water to generate MR images. However, the intrinsic MR contrast produced by water relaxation in organs and tissues is limited.

A B7H3-targeting antibody-drug conjugate in advanced solid tumors: a phase 1/1b trial.

Antibody-drug conjugates (ADCs) have emerged as a transformative modality in the treatment of solid tumors. YL201, a novel B7H3-targeting ADC, leverages a tumor microenvironment activable linker-payload platform, coupled with a novel topoisomerase 1 inhibitor via a protease-cleavable linker. Here we report the findings from a large-scale, global, multicenter, phase 1 trial evaluating the safety, pharmacokinetics and preliminary efficacy of YL201 in patients with advanced solid tumors refractory to standard therapies.

Gender-specific network analysis of parenting styles, depressive symptoms, and anxiety symptoms among 5157 Chinese adolescents.

Background: In this study, we aimed to use network analyses to explore the gender-specific interactions between individual items of depressive symptoms, anxiety symptoms, and parenting styles in Chinese adolescents.

Methods: We conducted a cross-sectional study with a total of 5157 adolescents in 5 primary schools, 8 junior high schools, and 2 senior high schools in Shandong province, China. Male and female adolescents' networks were assessed in all grades using the Egna Minnen Betraffande Uppfostran (EMBU), the 9-item Patient Health Questionnaire (PHQ-9), and the 7-item Generalized Anxiety Disorder Assessment (GAD-7).

Quantitative method for intestinal short chain fatty acids based on stable isotope labeling combined with liquid chromatography-mass spectrometry.

Short chain fatty acids (SCFAs) are produced from the breakdown of dietary proteins and fiber by gut microbes, and they have a close relationship with the health and diseases of the host. However, due to the similar structures of SCFAs, the abundance of active molecules, the wide concentration range in biological samples, and the characteristics such as high polarity, poor chromatographic separation, and ionization performance, it is challenging to comprehensively and accurately quantify SCFAs. This study utilized a stable isotope-labeled carboxyl derivatization reagent d-/d-2,4-dimethoxy-6-piperazin-1-yl pyrimidine (d-/d-DMPP) to establish a new method for the wide-coverage quantification analysis of SCFAs using ultra-high performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UHPLC-ESI-MS/MS), capable of detecting the content of 16 SCFAs.

The zinc finger transcription factor MtSTOP1 modulates aluminum resistance and low pH tolerance in Medicago truncatula.

In acidic soils, aluminum (Al) is a primary factor that inhibits plant growth. SENSITIVE-TO-PROTON-RHIZOTOXICITY1 (STOP1), which is accumulated in the nucleus in response to Al or low pH, regulates multiple downstream genes to counteract Al toxicity and acid stress. Here, we discovered four STOP1-like proteins in Medicago truncatula, which are localized in the nucleus and display transcriptional activity.

Recent advancements in wearable sensors: integration with machine learning for human-machine interaction.

Wearable sensors have emerged as a transformative technology, enabling real-time monitoring and advanced functionality in various fields, including healthcare, human-machine interaction, and environmental sensing. This review provides a comprehensive overview of the latest advancements in wearable sensor technologies, focusing on innovations in sensor design, material flexibility, and integration with machine learning. We explore the feasibility of wearable electronics in achieving high-performance, flexible devices and discuss their potential to enhance human-machine interactions through intelligent data processing and decision-making.

Daily physical activity, coffee and energy drink consumption, and sleep patterns among Chinese elite professional esports athletes: a case study of Zhejiang Regans Gaming.

Purpose: Esports is increasingly recognized as both a legitimate sport and profession. However, evidence on the health behaviors of esports athletes remains limited. Due to the nature of their activity, these athletes face occupational health risks associated with prolonged sedentary behavior, sleep deprivation, and excessive energy drink consumption.

The differential effects of integrase strand transfer inhibitors and efavirenz on neuropsychiatric conditions and brain imaging in HIV-positive men who have sex with men.

Integrase strand transfer inhibitors (INSTIs) have emerged as the first-line choice for treating human immunodeficiency virus (HIV) infection due to their superior efficacy and safety. However, the impact of INSTIs on the development of neuropsychiatric conditions in people living with HIV (PLWH) is not fully understood due to limited data. In this study, we conducted a cross-sectional examination of PLWH receiving antiretroviral therapy, with a specific focus on HIV-positive men who have sex with men (MSM) on INSTI-based regimens (n = 61) and efavirenz (EFV)-based regimens (n = 28).

Graphene-Based Far-Infrared Therapy Promotes Adipose Tissue Thermogenesis and UCP1 Activation to Combat Obesity in Mice.

Hyperthermia (HT) has broad potential for disease treatment and health maintenance. Previous studies have shown that far-infrared rays (FIRs) at 8-10 μm can potentially reduce inflammation, oxidative stress, and gut microbiota imbalance. However, the effects of FIR HT on energy metabolism require further investigation.

Acid Sphingomyelinase Regulates AdipoRon-Induced Differentiation of Arterial Smooth Muscle Cells via TFEB Activation.

AdipoRon is a selective adiponectin receptor agonist that inhibits vascular remodeling by promoting the differentiation of arterial smooth muscle cells (SMCs). Our recent studies have demonstrated that activation of TFEB and its downstream autophagy-lysosomal signaling contribute to adipoRon-induced differentiation of SMCs. The present study was designed to examine whether acid sphingomyelinase (ASM; gene symbol ) is involved in mediating adipoRon-induced activation of TFEB-autophagy signaling and inhibition of proliferation/migration in arterial SMCs.

Analysis of Risk Factors of Feather Pecking Injurious Behavior in Experimentally Raised Yangzhou Goslings in China.

Feather pecking can influence the welfare and health concerns of all farmed poultry and affect production and economic aspects. Although some information is available about feather pecking behavior in chickens and ducks, the risk factors of feather pecking in goslings have not been fully demonstrated. In this study, 3-day-old Yangzhou goslings were chosen, and risk factors of feather pecking injurious behavior were investigated, including stocking density, rearing method, flock uniformity, and environmental enrichment.

Fatty acid metabolism shapes immune responses in chronic lymphocytic leukemia.

Background: Fatty acids serve as a crucial energy source for tumor cells during the progression of chronic lymphocytic leukemia (CLL). The present study aims to elucidate the characteristics of fatty acid metabolism (FAM) in CLL, construct a related prognostic score, and investigate the regulatory role and mechanisms of FAM in CLL development.

Methods: Bulk RNA sequencing data from CLL patients and healthy controls were analyzed to identify differentially expressed fatty acid metabolic genes.

OMEGA-guided DNA polymerases enable random mutagenesis in a tunable window.

Targeted random mutagenesis is crucial for breeding, directed evolution, and gene function studies, yet efficient tools remain scarce. Here, we present obligate mobile element guided activity (OMEGA)-R, an innovative targeted random mutagenesis system that integrates SpyCatcher-enIscB and PolI3M-TBD-SpyTag, outperforming existing state-of-the-art technologies in key metrics, such as protein size, mutagenesis efficiency, window length, and continuity. OMEGA-R achieves a dramatic enhancement of on-target mutagenesis, reaching a rate of 1.

Exploration of crucial stromal risk genes associated with prognostic significance and chemotherapeutic opportunities in invasive ductal breast carcinoma.

Background: Few studies revealed that stromal genes regulate the tumor microenvironment (TME). However, identification of key-risk genes in the invasive ductal breast carcinoma-associated stroma (IDBCS) and their associations with the prediction of risk group remains lacking.

Methods: This study used the GSE9014, GSE10797, GSE8977, GSE33692, and TGGA BRCA datasets.

Spatiotemporal variations in runoff and sediment load of the mainstem and major tributaries of the Yellow River Basin from the headwater to the estuary (1960-2023).

Mastering the spatiotemporal variations and driving factors of runoff and sediment load (RS) in the Yellow River Basin (YRB) from the perspective of scientific governance is essential for formulating management strategies. Based on the clustering analysis, Pettitt abrupt change test, Mann-Kendall trend test, Sen's slope estimator, Wavelet analysis, and Double Mass Curve (DMC), This study analyzed the RS series from 32 mainstem and 34 major tributary hydrological stations across the YRB from 1960 to 2023, identified key driving factors, and quantified their attribution rates. The main findings were as follows: (1) The YRB was categorized into five segments (B1: upstream of Jimai, B2: Jimai-Tangnaihai, B3: Tangnaihai-Dengkou, B4: Dengkou-Longmen, B5: Longmen-Lijin) and three periods (P1: 1960-1986, P2: 1987-1999, P3: 2000-2023), with significant differences in RS distribution, particularly larger runoff in the upstream of Lanzhou and larger sediment load in the Toudaoguai-Tongguan.

A novel Adolescent Health Behavior Checklist.

Purpose: Adolescents are experiencing rising rates of obesity, insufficient exercise, and sleep disorders. To provide a scientific basis for policymakers to develop targeted and evidence-based health behavior education and policies, this study employed structural equation modeling to design the Adolescent Health Behavior Checklist (AHBC).

Methods: We designed a draft 6-dimensional AHBC, which includes the dimensions of exercise, diet, personal responsibility, sleep, interpersonal relationships, and stress management.

Near-infrared light-responsive nanocomposite hydrogels loaded with epidermal growth factor for diabetic wound healing.

In diabetic wounds, the presence of hyperglycemia is often accompanied by a persistent inflammatory response, oxidative stress damage, impaired angiogenesis and bacterial infections around the wound, resulting in impaired proliferation of dermal and epidermal cells and impaired skin regeneration in diabetic wounds. To solve the above problems, this study designed a near-infrared (NIR) light-responsive multifunctional poloxamer hydrogel (EGF/PDA-MXene Gel). The Gel is composed of two-dimensional nanomaterials (2D NMs) MXene as the core, modified by polymer, further loaded with epidermal growth factor (EGF), and has antibacterial, antioxidant, photothermal properties.

Targeting TRPV1 channels in desensitized neural afferent pathways may help mitigate pain and lower urinary tract symptoms caused by prostatitis.

Chronic prostatitis/chronic pelvic pain syndrome (CPPS/CP) is a prevalent urinary disorder primarily characterized by pelvic pain and discomfort, bladder dysfunction, and sexual dysfunction. Currently, there is no effective method to alleviate the pain and lower urinary tract symptoms associated with chronic prostatitis. Resiniferatoxin (RTX), a highly potent TRPV1 receptor agonist, functions as a molecular analgesic by desensitizing TRPV1-expressing nerves.