Identification of the role of SNARE proteins in rAAV vector production through interaction with the viral MAAP.

Adeno-associated virus (AAV) expresses a membrane-associated accessory protein (MAAP), a small nonstructural protein, that facilitates AAV secretion out of the plasma membrane through an association with extracellular vesicles during AAV egress. Here, we investigated the host proteins that interact with AAV2 MAAP (MAAP2) using APEX2-mediated proximity labeling. We identified two SNARE proteins, Syntaxin 7 (STX7) and synaptosome-associated protein 23 (SNAP23), a vesicle (v-)SNARE and a target (t-)SNARE, respectively, that mediate intracellular trafficking of membrane vesicles aand exhibited associations with MAAP2 in HEK293 cells.

Survival outcomes among periviable infants: a systematic review and meta-analysis comparing different income countries and time periods.

Background: Periviable infants are a highly vulnerable neonatal group, and their survival rates are considerably affected by patient-, caregiver-, and institution-level factors, exhibiting wide variability across different income countries and time periods. This study aims to systematically review the literature on the survival rates of periviable infants and compare rates among countries with varied income levels and across different time periods.

Methods: Comprehensive searches were conducted across MEDLINE, Embase, CENTRAL, and Web of Science.

Spatial frequency of environments and myopia: A systematic review on associated evidence and underlying mechanisms.

Purpose: Previous animal studies have found a relationship between spatial frequency and myopia. New research in humans suggest that reduced high spatial content of the visual environment may be a contributing factor for myopia development. This study aims to review the literature and elucidate the potential biological mechanisms linking spatial frequency and myopia.

3D-printed acoustic metasurface with encapsulated micro-air-bubbles for frequency-selective manipulation.

Acoustic waves provide an effective method for object manipulation in microfluidics, often requiring high-frequency ultrasound in the megahertz range when directly handling microsized objects, which can be costly. Micro-air-bubbles in water offer a solution toward low-cost technologies using low-frequency acoustic waves. Owing to their high compressibility and low elastic modulus, these bubbles can exhibit significant expansion and contraction in response to even kilohertz acoustic waves, leading to resonances with frequencies determined and tuned by air-bubble size.

Analysis of deviant behaviors and family functions in the population at risk of internet addiction among primary and secondary school students in Chengdu city, Sichuan province of China.

Background: This study explores the correlation between internet addiction (IA) and deviant behaviors among primary and secondary school students. It analyzes the impact of family functional factors, such as family relationships, parent-child communication, and parental control, on IA. The findings aim to provide a scientific basis for educators and parents to develop targeted preventive and intervention measures.

[Characterization of the Community Structure of Rhizosphere Soil and Root-endophytic Bacteria in Different Shrubs].

In the process of ecological restoration, vegetation plays a crucial role in restoring ecosystem functions. Soil microorganisms are essential components of soil ecosystems, driving material cycling processes and enhancing plant productivity and resilience. This study aimed to investigate the community structure characteristics of rhizosphere soil and root-endophytic bacteria in different shrubs.

Phosphorylation determines the glucose metabolism reprogramming and tumor-promoting activity of sine oculis homeobox 1.

Aerobic glycolysis is a hallmark of cancer and is regulated by growth factors, protein kinases and transcription factors. However, it remains poorly understood how these components interact to regulate aerobic glycolysis coordinately. Here, we show that sine oculis homeobox 1 (SIX1) phosphorylation integrates growth factors (e.

Identification of SLC35A1 as an essential host factor for the transduction of multi-serotype recombinant adeno-associated virus (AAV) vectors.

We conducted a genome-wide CRISPR/Cas9 screen in suspension 293 F cells transduced with rAAV5. The highly selected genes revealed after two rounds of screening included the previously reported , , and , along with a cluster of genes involved in glycan biogenesis, Golgi apparatus localization, and endoplasmic reticulum penetration. In this report, we focused on solute carrier family 35 member A1 (), a Golgi apparatus-localized cytidine 5'-monophosphate-sialic acid (CMP-SIA) transporter.

fabrication of self-filtered near-infrared TiCT/n-Si Schottky-barrier photodiodes for a continuous non-invasive photoplethysmographic system.

Two-dimensional (2D) MXenes have emerged as promising candidates to serve as Schottky contact electrodes for the development of high-performance photodiodes owing to their extraordinary electronic properties. However, it remains a formidable challenge to fabricate a large-area, uniform MXene layer for practical device application. Here, we develop a facile route to produce a large-area TiCT layer by post-etching treatment of a pulsed laser-deposited TiAlC film, enabling the construction of a back-illuminated TiCT/n-Si Schottky-barrier photodiode.

Jade powder/PLGA composite microspheres for improved performance as potential bone repair drug carrier.

Poly (lactic-co-glycolic acid) (PLGA) has been widely used as drug delivery carrier or scaffold for bone repair due to its good biocompatibility, biodegradability, and degradation rate controllability. However, defects, like acidic degradation by-products, are associated with PLGA and restrict its practical applications. Jade powder, leftover from jade polishing process, is a natural material rich in elements of Ca, Si, and Mg while biocompatible and antibacterial.

Fish promotes viral products by modulating the innate immune response and exosomal machinery.

Viral nervous necrosis caused by the nervous necrosis virus (NNV) poses a significant threat to the global aquaculture industry. Developing preventive methods to minimize economic losses due to NNV infections is crucial. This study explored the role of the sorting nexin 27 () gene, encoded by the orange-spotted grouper () and referred to as , as an immune regulator affecting red-spotted grouper nervous necrosis virus (RGNNV) infection .

Identification of SLC35A1 as an essential host factor for the transduction of multi-serotype recombinant adeno-associated virus (AAV) vectors.

We conducted a genome-wide CRISPR/Cas9 screen in suspension 293-F cells transduced with rAAV5. The highly selected genes revealed after two rounds of screens included the previously reported , , and , along with a cluster of genes involved in glycan biogenesis, Golgi apparatus localization and endoplasmic reticulum penetration. In this report, we focused on solute carrier family 35 member A1 (), a Golgi apparatus-localized cytidine 5'-monophosphate-sialic acid (CMP-SIA) transporter.

Fabrication of polyoxometalate dispersed cobalt oxide nanowires for electrochemically monitoring superoxide radicals from Hela cell mitochondria.

An ultrasensitive electrochemical sensor is constructed by electrostatically adsorbing negatively charged hourglass-shape Cu-Polyoxometalate (POM) onto a positively charged CoO nanowires modified carbon cloth. The petaloid CoO nanowires have a large specific surface area that can well disperse open-structured Cu-POM to form Cu-POM@CoONWs@CC, which can maximumly expose catalytic active centers (Co and Cu) and accelerate mass/charge transfer. In addition to the above advantages, the excellent electron exchange ability of Cu-POM and good conductivity of CoONWs@CC endow the sensor with good detection capability to HO including a linear detection range of 0.

Proteomics and Microbiota Conjoint Analysis in the Nasal Mucus: Revelation of Differences in Immunological Function in and .

All eight pangolin species, especially captive , are critically endangered and susceptible to various pathogenic microorganisms, causing mass mortality. They are involved in the complement system, iron transport system, and inflammatory factors. exhibited a higher abundance of opportunistic pathogens, , which potentially evaded complement-mediated immune response by reducing C5 levels and counteracting detrimental effects through transferrin neutralization.

Transcriptional Regulation of De Novo Lipogenesis by SIX1 in Liver Cancer Cells.

De novo lipogenesis (DNL), a hallmark of cancer, facilitates tumor growth and metastasis. Therapeutic drugs targeting DNL are being developed. However, how DNL is directly regulated in cancer remains largely unknown.

Phosphomolybdates for Dual-Mode Photoelectrochemical Sensing toward Trace Chromium(VI) and Tetracycline.

Highly sensitive photoelectrochemical (PEC) sensors for trace carcinogens, such as heavy metal chromium(VI) [Cr(VI)] and antibiotic tetracycline (TC) are crucial. Herein, by integration of photoactive and redox phosphomolybdates with conjugated organic components, types of dual-mode PEC sensors were synthesized for sensing trace Cr(VI) and TC pollutants, with formulas of (Hbimb)[Co(bimb)][Co(HO)][Co(PMoOH)]·6HO (), (Hbib)[Co(HO)][Co(HO)][Co(PMoOH)]·9HO (), and (Hbib)[Co(Hbib)(HO)][Co(PMoOH)]·15HO (), where bimb represents 1,4-bis(1-imidazolyl)benzene and bib is 4,4'-bis(imidazolyl)bibphenyl. Hybrid consisted of a three-dimensional framework structure constructed by Co{PMo} clusters and one-dimensional (1D) {Co-bimb} chains, hybrid exhibited 1D Co ion-bridged Co{PMo} chains hydrogen-bonding with [Hbib] cations, and hybrid showed a discrete hybrid structure built upon a Co{PMo} cluster modified by the {Co-bib} unit.

Artificial organic afferent nerves enable closed-loop tactile feedback for intelligent robot.

The emulation of tactile sensory nerves to achieve advanced sensory functions in robotics with artificial intelligence is of great interest. However, such devices remain bulky and lack reliable competence to functionalize further synaptic devices with proprioceptive feedback. Here, we report an artificial organic afferent nerve with low operating bias (-0.

Expressions and clinical significance of CCN5 and E-cadherin in primary and recurrent lesions of breast cancer.

Background: Breast cancer recurrence and lymph node metastasis significantly impact patient outcomes. Understanding the molecular mechanisms behind these processes is crucial for developing effective treatments. CCN5 and E-cadherin are proteins involved in cell adhesion and epithelial-mesenchymal transition (EMT), playing roles in breast cancer progression.

A deep learning system for myopia onset prediction and intervention effectiveness evaluation in children.

The increasing prevalence of myopia worldwide presents a significant public health challenge. A key strategy to combat myopia is with early detection and prediction in children as such examination allows for effective intervention using readily accessible imaging technique. To this end, we introduced DeepMyopia, an artificial intelligence (AI)-enabled decision support system to detect and predict myopia onset and facilitate targeted interventions for children at risk using routine retinal fundus images.

Risk factors for vaginal squamous intra-epithelial lesions in women with high-grade cervical lesions.

Objective: To investigate the high-risk factors associated with concurrent cervical intra-epithelial neoplasia (CIN) and vaginal intra-epithelial neoplasia (VaIN) in patients with high-grade lesions.

Methods: This retrospective study at the Obstetrics and Gynecology Hospital of Fudan University included patients diagnosed with concurrent CIN2/3 and VaIN2/3 (concurrent group) over the period from January 1, 2019, to December 31, 2019. Patients with only CIN2/3 during the corresponding period were selected chronologically on a 1:2 basis (CIN group).

PDZD8 Augments Endoplasmic Reticulum-Mitochondria Contact and Regulates Ca2+ Dynamics and Cypd Expression to Induce Pancreatic β-Cell Death during Diabetes.

Backgruound: Diabetes mellitus (DM) is a chronic metabolic disease that poses serious threats to human physical and mental health worldwide. The PDZ domain-containing 8 (PDZD8) protein mediates mitochondria-associated endoplasmic reticulum (ER) membrane (MAM) formation in mammals. We explored the role of PDZD8 in DM and investigated its potential mechanism of action.