ML216-Induced BLM Helicase Inhibition Sensitizes PCa Cells to the DNA-Crosslinking Agent Cisplatin.

Using standard DNA-damaging medicines with DNA repair inhibitors is a promising anticancer tool to achieve better therapeutic responses and reduce therapy-related side effects. Cell viability assay, neutral comet assay, western blotting (WB), and cell cycle and apoptosis analysis were used to determine the synergistic effect and mechanism of ML216, a Bloom syndrome protein (BLM) helicase inhibitor, and cisplatin (CDDP), a DNA-crosslinking agent, in PCa cells. Based on the online database research, our findings revealed that BLM was substantially expressed in PCa, which is associated with a bad prognosis for PCa patients.

Specific immunoassay reveals increased serum trypsinogen 3 in acute pancreatitis.

Background: Trypsinogen 3 is a minor trypsinogen isoform in the pancreas. In contrast with trypsin 1 and 2, trypsin 3 degrades pancreatic secretory trypsin inhibitor, which may lead to an excess of active trypsin and acute pancreatitis (AP). We developed an immunoassay for trypsinogen 3 and studied whether an assay of serum trypsinogen 3 is of clinical utility in the diagnosis of AP.

Human umbilical cord-derived Schwann-like cell transplantation combined with neurotrophin-3 administration in dyskinesia of rats with spinal cord injury.

Mesenchymal stem cells are capable of differentiating into Schwann-like cells. In this study, we induced human umbilical-cord mesenchymal stem cells (HUMSCs) in vitro into neurospheres constituted by neural stem-like cells, and further into cells bearing strong morphological, phenotypic and functional resemblances with Schwann-like cells. These HUMSC-derived Schwann-like cells, after grafting into the injured area of the rats' spinal cord injury (SCI), showed a partial therapeutic effect in terms of improving the motor function.

Studies of the neural mechanisms of deep brain stimulation in rodent models of Parkinson's disease.

Several rodent models of deep brain stimulation (DBS) have been developed in recent years. Electrophysiological and neurochemical studies have been performed to examine the mechanisms underlying the effects of DBS. In vitro studies have provided deep insights into the role of ion channels in response to brain stimulation.

Studies of the neural mechanisms of deep brain stimulation in rodent models of Parkinson's disease.

Several rodent models of deep brain stimulation (DBS) have been developed in recent years. Electrophysiological and neurochemical studies have been performed to examine the mechanisms underlying the effects of DBS. In vitro studies have provided deep insights into the role of ion channels in response to brain stimulation.

[Pituitary abscess in a child].

As a clinically rare condition, pituitary abscess lacks a definite history of infection or characteristic symptoms such as fever or meningismus for a definite diagnosis. From the experience with a case of pituitary abscess in a child, we urged that attention should be given to differentiation of this condition from cystoid changes of the pituitary tumor, craniopharyngioma, Rothka cyst, sphenoidal sinus cyst and sphenoidal sinus abscess.

[Protective effect of exogenous superoxide dismutase against rat midbrain dopaminergic neuron apoptosis induced by 6-hydroxydopamine].

Objective: To observe the changes of total antioxidant ability of rat midbrain and T-superoxide dismutase (T-SOD) activity, and define the relation between oxidative stress and dopaminergic neuron apoptosis induced by 6-hydroxydopamine (6-OHDA), observing also the protection of the exogenous SOD against the neuron apoptosis.

Methods: A total of 144 SD rats was randomized into normal control, model control, model, treatment control and treatment groups according to different treatments the rats received. The treatment group was subdivided into A, B, C, D and E groups with different treating times after model establishment.

Long-term high-frequency electro-acupuncture stimulation prevents neuronal degeneration and up-regulates BDNF mRNA in the substantia nigra and ventral tegmental area following medial forebrain bundle axotomy.

Electroacupuncture (EA) has been used in China for many years to treat Parkinson's disease (PD) with reportedly effective results. However, the physiological and biological mechanism behind its effectiveness is still unknown. In the present study, different frequencies of chronic EA stimulation (0, 2, 100 Hz) were tested in a partially lesioned rat model of PD which was induced by transection of the medial forebrain bundle (MFB).