Tetra-proline-modified calix[4]arene-bonded silica stationary phase for simultaneous reversed-phase/hydrophilic interaction mixed-mode chromatography.

A new water-soluble tetra-proline-modified calix[4]arene-bonded silica stationary phase was prepared straightforwardly by an indirect method and characterized by elemental analysis, energy dispersive Spectrometry, solid-state C NMR spectroscopy, Fourier-transform infrared spectroscopy, and thermogravimetric analysis. Due to the simultaneous introduction of polar tetra-proline and nonpolar calix[4]arene, the developed column possessing a double retention mode of reverse-phase liquid chromatography and hydrophilic interaction liquid chromatography. A series of hydrophobic and hydrophilic test samples, including nucleosides and nucleotides, amines, monosubstituted benzenes, chiral compounds, and phenols, were used to evaluate the developed stationary phase.

Asymmetric Alkenylation of Enones and Imines Enabled by A Highly Efficient Aryl to Vinyl 1,4-Rhodium Migration.

The asymmetric rhodium-catalyzed alkenylation of enones and imines with arylboronic acids has been developed. A highly controllable aryl to vinyl 1,4-rhodium migration is the key step. Stereodefined vinyl moieties were installed in excellent enantioselectivies for most examined examples.

Ultrasensitive SERS detection of highly homologous miRNAs by generating 3D organic-nanoclusters and a functionalized chip with locked nucleic acid probes.

Herein, a new finding is reported that 3D organic-nanoclusters (3DONs) with superior SERS properties as an original reporter could accurately and sensitively distinguish microRNAs (miRNAs) with highly similar sequences, even with a single-nucleotide difference, as a result of a functionalized chip with locked nucleic acid probes (LNAP-chip) and rolling circle replication (RCA). Furthermore, the innovative SERS-method could be used to broadly discriminate cancer cells from normal cells and evaluate changes in the expression levels of intracellular miRNAs.

Tetra-proline modified calix[4]arene bonded silica gel: A novel stationary phase for hydrophilic interaction liquid chromatography.

Calixarenes and their derivatives have been widely used in chromatographic studies due to unique host-guest recognition properties. However, stationary phases in hydrophilic interaction chromatography associated with them have yet to be studied because of their general hydrophobicity. In this paper, a tetra-proline modified calix[4]arene bonded stationary phase (DTPCSP) was prepared and characterized by FT-IR spectra, elemental analysis, solid state C NMR, SEM, EDS and thermogravimetric analysis.

Solid-phase extraction of aflatoxins using a nanosorbent consisting of a magnetized nanoporous carbon core coated with a molecularly imprinted polymer.

A core consisting of nanoporous carbon (MNPC) and magnetized with CoO was coated with a molecularly imprinted polymer (MIP) by atom transfer radical precipitation polymerization. Ethyl 3-coumarincarboxylate was used as a pseudo-template to give a MIP that has a fairly specific recognition capability for aflatoxins. Batch rebinding studies were carried out to determine the specific adsorption equilibrium and specific recognition.

Exploits CD209 Receptors for Promoting Host Dissemination and Infection.

is a Gram-negative enteropathogen and causes gastrointestinal infections. It disseminates from gut to mesenteric lymph nodes (MLNs), spleen, and liver of infected humans and animals. Although the molecular mechanisms for dissemination and infection are unclear, many Gram-negative enteropathogens presumably invade the small intestine via Peyer's patches to initiate dissemination.

Inkjet-Printing Patterned Chip on Sticky Superhydrophobic Surface for High-Efficiency Single-Cell Array Trapping and Real-Time Observation of Cellular Apoptosis.

Single-cell assays have broad applications in cellular studies, tissue engineering, fundamental studies of cell-cell interactions, and understanding of cell-to-cell variations. Most existing methods for micron-sized cell patterning are still based on lithography-based microfabrication process. Thus, exploiting new mask-free strategies while maintaining high-precision single-cell patterning is still a great challenge.

Synthesis of cobalt-based magnetic nanoporous carbon core-shell molecularly imprinted polymers for the solid-phase extraction of phthalate plasticizers in edible oil.

In this work, cobalt magnetic nanoporous carbon (Co-MNPC) is employed as an alternative to intensively used FeO cores for the preparation of magnetic molecularly imprinted polymers (Co-MNPC@MIPs) for the first time. Co-MNPC was prepared by one-step carbonization of Zeolitic Imidazolate Framework-67 (ZIF-67). Compared with the traditional FeO core, Co-MNPC showed a high specific surface area and large pore volumes.

Abietane Diterpenoids from the Roots of Clerodendrum trichotomum and Their Nitric Oxide Inhibitory Activities.

Twelve new abietane diterpenoids (1-12) and 31 known analogues (13-43) were isolated from a medicinal Chinese herb, Clerodendrum trichotomum Thunberg. The absolute configurations of 1-3 were established on the basis of ECD and X-ray crystallography data, whereas that of 4 was elucidated by comparison of experimental and calculated ECD data. Eight diterpenoids, 15,16-dehydroteuvincenone G (1), trichotomin A (4), 2α-hydrocaryopincaolide F (7), villosin C (20), 15-dehydro-17-hydroxycyrtophyllone A (22), demethylcryptojaponol (38), 6β-hydroxydemethylcryptojaponol (39), and trichotomone (43), exerted inhibitory effects against NO production with IC values of 5.

Determination of benzo[α]pyrene in edible oil using tetraoxocalix[2]arene[2]triazine bonded silica SPE sorbent.

Benzo[α]pyrene (BaP) is a well-known carcinogen in edible oil. In this study, a method combined solid-phase extraction (SPE) with fluorescent detection was developed using tetraoxocalix[2]arene[2]triazine sorbent (SiO-OCA) for the clean-up and enrichment of BaP. The interaction between SiO-OCA and BaP involves a donor-acceptor complex mechanism.

In situ preparation of core-shell magnetic porous aromatic framework nanoparticles for mixed-mode solid-phase extraction of trace multitarget analytes.

This study reports a novel strategy for the preparation of porous aromatic framework (PAF-6) coated magnetic nanoparticles (PAF-6 MNPs) using cyanuric chloride as a planar trigonal basis upon which to build a linear piperazine linker unit. The PAF-6 MNPs were examined as an efficient solid-phase extraction (SPE) sorbent for enrichment of trace organic pollutants including phenol, 2,4,6-trinitrophenol, naphthalene, naphthol, bisphenol A, 2,4-dichlorophenol and 3-nitrochlorobenzene in water. The high-performance liquid chromatography detection limits of such analytes were in the range of 0.

The complete mitochondrial genome of (Ephemeroptera: Isonychiidae).

The complete mitochondrial genome of is a circular molecule of 15,456 bp in length, containing 2 rRNA genes, 13 protein-coding genes, 22 tRNA genes, and a control region. The AT content of the overall base composition is 62.9%.

Determination of oxalate and citrate in urine by capillary electrophoresis using solid-phase extraction and capacitively coupled contactless conductivity based on an improved mini-cell.

A new method for the rapid determination of the metabolites oxalate and citrate in urine samples was based on capillary electrophoresis and capacitively coupled contactless conductivity detection coupled with solid-phase extraction. The detection cell for capacitively coupled contactless conductivity detection was improved with a smaller inner volume (1.5 nL), reduced noise (0.

Integration of intracellular telomerase monitoring by electrochemiluminescence technology and targeted cancer therapy by reactive oxygen species.

Cancer therapies based on reactive oxygen species (ROS) have emerged as promising clinical treatments. Electrochemiluminescence (ECL) technology has also attracted considerable attention in the field of clinical diagnosis. However, studies about the integration of ECL diagnosis and ROS cancer therapy are very rare.

Simultaneous quantification of three alkylated‑purine adducts in human urine using sulfonic acid poly(glycidyl methacrylate‑divinylbenzene)-based microspheres as sorbent combined with LC-MS/MS.

Three alkylated DNA adducts, N‑methyladenine, N‑ethyladenine and N‑ethylguanine, have been proved to be potential biomarkers for DNA injury caused by exposure to cigarette smoke. In this study, a highly specific and sensitive method using a new mixed-mode sulfonate-functionalized poly(glycidyl methacrylate-divinylbenzene) as a solid-phase extraction sorbent was developed for the analysis of these three alkylated-purine adducts in human urine. Under optimized conditions, the prepared sorbent interacts strongly with these urinary adducts, demonstrating high clean-up efficiency and extraction recovery.

Multicolor Upconversion Nanoprobes Based on a Dual Luminescence Resonance Energy Transfer Assay for Simultaneous Detection and Bioimaging of [Ca ] and pH in Living Cells.

Intracellular [Ca ] and pH have a close relationship, and their abnormal levels can result in cell dysfunction and accompanying diseases. Thus, simultaneous determination of [Ca ] and pH can more accurately investigate complex biological processes in an integrated platform. Herein, multicolor upconversion nanoparticles (UCNPs) were prepared with the advantages of no spectral overlapping, single NIR excitation wavelengths, and greater tissue penetration depth.

The complete mitochondrial genome of (Ephemeroptera: Heptageniidae) and its phylogeny.

The mitochondrial genome of (Ephemeroptera: Heptageniidae) is a circular molecule of 15,801 bp in length with a base composition of 32.7% A, 32.9% T, 21.

Cardamonin enhances the anti-proliferative effect of cisplatin on ovarian cancer.

The mammalian target of rapamycin (mTOR) is well-known as a promising therapeutic target in various cancer cells. mTOR activation decreases the sensitivity of ovarian cancer to cisplatin. Cardamonin inhibits the proliferation of various cancer cells by mTOR suppression.

Optical nano-biosensing interface via nucleic acid amplification strategy: construction and application.

Modern optical detection technology plays a critical role in current clinical detection due to its high sensitivity and accuracy. However, higher requirements such as extremely high detection sensitivity have been put forward due to the clinical needs for the early finding and diagnosing of malignant tumors which are significant for tumor therapy. The technology of isothermal amplification with nucleic acids opens up avenues for meeting this requirement.

Investigation of the chromatographic regulation properties of benzyl groups attached to bridging nitrogen atoms in a calixtriazine-bonded stationary phase.

Two reversed-phase/anion-exchange mixed-mode stationary phases for high-performance liquid chromatography using calixtriazines as chromatographic ligands were investigated with Tanaka test solutes, monosubstituted benzenes, aromatic positional isomers, and inorganic anions. Calixtriazine as a chromatographic ligand has been reported previously, but the benzylated nitrogen-bridged calixtriazine-bonded silica gel reported in this study is new. The experimental data showed that the calixtriazine platform is a unique chromatographic selector because its multiple active sites are available for different solutes and its chromatographic selectivity could be tuned by introducing substituent on the bridging nitrogen atoms present in the calixtriazine matrix.

Functionalized metal-organic framework nanocomposites for dispersive solid phase extraction and enantioselective capture of chiral drug intermediates.

The facile preparation, characterization and application of a novel magnetic graphene oxide- metal organic framework [Zn(d-Cam)(4, 4'-bpy)] (MGO-ZnCB) as a sorbent for fast, simple and enantioselective capture of chiral drug intermediates are presented in this paper. The MGO-ZnCB nanocomposite, developed by encapsulating MGO nanoparticles into the homochiral metal organic framework of ZnCB, can integrate the advantages from each component endowing the hybrids with improved synergystic effects. The enantioselective performance of MGO-ZnCB was evaluated by dispersive magnetic nanoparticle solid phase extraction (d-MNSPE) of 1, 1'-bi-2-naphthol (BN) and 2, 2'-furoin (Furoin) racemic solutions.

Proximity ligation detection of lectin Concanavalin A and fluorescence imaging cancer cells using carbohydrate functionalized DNA-silver nanocluster probes.

A novel method for study the interaction between carbohydrate and lectin was developed in homogenous solution. DNA was introduced to the recognition process of carbohydrate with protein. Due to the versatile detection mode of DNA, the detection efficiency for carbohydrate and protein was highly promoted.

Ultrasensitive Detection of Cancer Cells Combining Enzymatic Signal Amplification with an Aerolysin Nanopore.

Sensitive detection of cancer cells at extremely low concentrations would greatly facilitate the screening and early diagnosis of cancer. Herein, we present a novel nanopore-based strategy for ultrasensitive detection of Ramos cells (human Burkitt's lymphoma cells), by combining the enzymatic signal amplification with an aerolysin nanopore sensor. In this assay, an aptamer for Ramos cells was prehybridized with a short complementary DNA.

Urinary metal and metalloid biomarker study of Henoch-Schonlein purpura nephritis using inductively coupled plasma orthogonal acceleration time-of-flight mass spectrometry.

To obtain a better understanding as to whether concentration alterations of metals and metalloids in urine were related to Henoch-Schonlein purpura nephritis (HSPN), the profiles of as many as 29 elements in urine were compared among three groups, the Henoch-Schonlein purpura (HSP), HSPN and a healthy control group. To this end, a reliable method has been developed for the simultaneous quantification of multiple elements including Li, Be, B, Al, Sc, Ti, V, Cr, Mn, Fe, Ni, Co, Cu, Zn, Ga, Ge, As, Se, Rb, Sr, Mo, Cd, Sn, Sb, Cs, Ba, Tl, Pb and Bi in urine using inductively coupled plasma orthogonal acceleration time-of-flight mass spectrometry (ICP-oa-TOF-MS). The process of sample pre-treatment used a direct 20-fold dilution method with centrifuged urine.

Application of pseudo-template molecularly imprinted polymers by atom transfer radical polymerization to the solid-phase extraction of pyrethroids.

A polymer-based adsorption medium with molecular recognition ability for homologs of pyrethroids was prepared by atom transfer radical polymer iration using a fragment imprinting technique. Phenyl ether-biphenyl eutectic was utilized as a pseudo-template molecule, and the adsorption medium prepared was evaluated by solid-phase extraction and gas chromatography. Selectivity of the medium for pyrethroids was evaluated using it as solid phase extraction packing by Gas Chromatography.