Genome-scale metabolic modelling reveals interactions and key roles of symbiont clades in a sponge holobiont.

Sponges harbour complex microbiomes and as ancient metazoans and important ecosystem players are emerging as powerful models to understand the evolution and ecology of symbiotic interactions. Metagenomic studies have previously described the functional features of sponge symbionts, however, little is known about the metabolic interactions and processes that occur under different environmental conditions. To address this issue, we construct here constraint-based, genome-scale metabolic networks for the microbiome of the sponge Stylissa sp.

UV-B induced flavonoid accumulation and related gene expression in blue- grained wheat at different periods of time.

Introduction: UV-B can be used as an additional technique for nutrient accumulation in blue-grained wheat, which has special nutritional properties due to its blue starch layer. The concentration of flavonoids in blue-grained wheat under UV-B irradiation is extremely important for further investigation and exploitation of the nutritional properties of blue-grained wheat.

Methods: This investigation focuses on the expression of flavonoids and associated genes in blue-grained wheat using transcriptomic and metabolomic analyzes.

Extraction, purification, structural characterization, and bioactivities of Radix Aconiti Lateralis Preparata (Fuzi) polysaccharides: A review.

Radix Aconiti Lateralis Preparata (Fuzi) polysaccharide (FZP) is a key bioactive macromolecule derived from the root of Aconitum carmichaeli Debx. FZP has a variety of biological activities, including immunomodulatory, anti-tumor, anti-depressant, organ-protective, hypoglycemic, anti-inflammatory, and other activities. The biological activities of polysaccharides are closely related to their structures, and different extraction and purification methods will yield different polysaccharide structures.

Prevalence and risk factors of pre-frailty and frailty in hemodialysis patients in central China.

The current study was to explore the prevalence and risk factors elements of pre-frailty and frailty among patients undergoing hemodialysis (HD) in central China. A cross-sectional, multi-institutional investigation was conducted. From March to May 2024, using the convenience sampling method, a total of 408 HD patients from four hospitals in Xiangyang, China, were recruited for this study.

Peripheral, central, and chemotherapy-induced neuropathic changes in pancreatic cancer.

In pancreatic cancer, significant alterations occur in the local nervous system, including axonogenesis, neural remodeling, perineural invasion, and perineural neuritis. Pancreatic cancer can impact the central nervous system (CNS) through cancer cell-intrinsic factors or systemic factors, particularly in the context of cancer cachexia. These peripheral and central neuropathic changes exert substantial influence on cancer initiation and progression.

Reconfigurations of dynamic functional network connectivity after 1HZ repetitive transcranial magnetic stimulation in insomnia disorder.

Aims: The objective of the current study was to investigate the dynamic functional connectivity among large-scale brain networks in patients with insomnia, and to assess the efficacy of repetitive transcranial magnetic stimulation (rTMS) treatment in these individuals.

Methods: Resting-state functional magnetic resonance imaging (rs-fMRI) data from 62 insomnia patients and 69 healthy controls were used to compare differences in dynamic functional connectivity between the two groups. A total of 26 insomnia patients underwent rTMS for four weeks.

FeCu-N6-C Diatomic Sites Catalyst for the Boosted Oxygen Reduction Reactions in Zinc-air Batteries.

Due to the high catalytic activity and stability for oxygen reduction reaction, N-coordinated Fe-Cu dual-metal doped carbon material (FeCu-N-C) is considered to be one of the promising electrode materials for metal-air battery and fuel cells. Herein, FeCu-N-C dual-metal catalysts was synthesized by an adsorption-calcination strategy. The prepared FeCu-N-C exhibited high activity and stability both in alkaline and acidic media.

Telitacicept for refractory cystitis associated with severe systemic lupus erythematosus: A case report.

Background: Lupus cystitis, a severe complication of systemic lupus erythematosus (SLE), presents considerable treatment challenges.

Purpose: This case report describes the use of telitacicept in treating severe SLE with lupus cystitis.

Research Design: A single patient with lupus cystitis.

Exploring the interplay of academic stress, motivation, emotional intelligence, and mindfulness in higher education: a longitudinal cross-lagged panel model approach.

Students in higher education often encounter significant academic pressure, which can have profound implications for their mental health and academic performance. The current study employs a two-wave longitudinal design to investigate the dynamic interrelationships among academic stress, academic motivation, emotional intelligence, and mindfulness. The study employed a cross-lagged panel model to investigate the temporal interactions among these four constructs and their influence on the academic experiences of doctoral students.

Bisulfite-Free and Quantitative Detection of DNA Methylation at Single-Base Resolution by eROS1-seq.

5-Methylcytosine (5mC) is the most significant DNA modification present in mammalian genomes. Understanding the roles of 5mC in diverse biological processes requires quantitative detection at single-base resolution. In this study, we engineered the repressor of the silencing 1 (ROS1) protein derived from to enhance its 5mC glycosylase/lyase activity, resulting in the creation of the engineered ROS1 (eROS1) protein.

Sustained Aesthetic Improvement in Midface Micro-Autologous Fat Transplantation Auxiliary Support on Lower Eyelid Rejuvenation: A Comprehensive Evaluation.

Purpose: To evaluate the deformities of tear troughs, and contours of the infraorbital-midfacial region, and to observe the clinical therapeutic effects of lower eyelid reconstruction combined with micro-autologous fat transplantation in orbital-midfacial rejuvenation.

Methods: A total of 87 individuals seeking cosmetic improvement, aged 25 to 45 years (mean age 36.7 years), were selected for this study from March 2020 to March 2022.

An Fe-doped Ni-based oxalate framework with a favorable electronic structure for electrocatalytic water and urea oxidation.

An Fe-doped Ni-based oxalate framework, synthesized a facile co-precipitation method, is applied as an excellent bi-functional electrocatalyst for water and urea oxidation reactions. The obtained framework achieved a large current density of 100 mA cm at 1.497 V and 1.

Scutellarin: pharmacological effects and therapeutic mechanisms in chronic diseases.

Scutellarin (SCU), a flavonoid glucuronide derived from and , exhibits broad pharmacological effects with promising therapeutic potential in treating various chronic diseases. It has demonstrated efficacy in modulating multiple biological pathways, including antioxidant, anti-inflammatory, anti-apoptotic, and vasodilatory mechanisms. These protective roles make SCU a valuable compound in treating chronic diseases such as cerebrovascular diseases, cardiovascular diseases, neurodegenerative disorders, and metabolic diseases.

New insights into the role of lipids in aroma formation during black tea processing revealed by integrated lipidomics and volatolomics.

Lipids are important tea aroma precursors. Due to the complexity of black tea processing involving both enzymatic and thermal reactions, the role of lipids in black tea aroma formation remained unclear. Herein, the dynamic changes of lipids and volatiles during black tea processing were simultaneously analyzed by lipidomics and volatolomics using ultra-high-performance liquid chromatography coupled to Q-Exactive Orbitrap mass spectrometry (UHPLC-Q-Exactive) and gas chromatography-tandem mass spectrometry (GC-MS/MS).

AIGen: an artificial intelligence software for complex genetic data analysis.

The recent development of artificial intelligence (AI) technology, especially the advance of deep neural network (DNN) technology, has revolutionized many fields. While DNN plays a central role in modern AI technology, it has rarely been used in genetic data analysis due to analytical and computational challenges brought by high-dimensional genetic data and an increasing number of samples. To facilitate the use of AI in genetic data analysis, we developed a C++ package, AIGen, based on two newly developed neural networks (i.

Oxide-Metal Hybrid Glass Nanomembranes with Exceptional Thermal Stability.

Contrary to oxide or polymeric glasses, metallic glasses are infamously known for their relatively limited thermal stability, which is often characterized by their narrow supercooled liquid regions. Nonetheless, we successfully fabricated metallic-glass based nanomembranes with an ultrahigh thermal ability by a polymer surface buckling enabled exfoliation technique. These nanomembranes exhibit a distinctive nanostructure with nanosized metallic-glasses encapsulated within an interconnected nanoamorphous-oxide network.

The value of the '7E' instructional model in the teaching of nursing students in nursing clinical probation.

Objective: To investigate the value of 7E instructional model in the teaching of nursing students in nursing clinical probation.

Methods: One hundred and fifty-nine nursing undergraduates of the class of 2018 who were on probation in our hospital from November 2020 to June 2021 were selected as the control group by convenience sampling, and the traditional teaching model was adopted; an additional 171 nursing undergraduates of the class of 2019 who were on clinical probation in the same hospital from November 2021 to June 2022 were selected as the experimental group, and the 7E instructional model was adopted, including elicit, engagement, exploration, explain, elaborate, evaluate and extend.

Results: After the intervention, the experimental group was higher than the control group in theoretical knowledge scores (59.