Ribavirin inhibits cell proliferation and metastasis and prolongs survival in soft tissue sarcomas by downregulating both protein arginine methyltransferases 1 and 5.

Protein arginine methyltransferases 1 and 5 (PRMT1 and PRMT5) are frequently overexpressed in diverse types of cancers and correlate with poor prognosis, thus making these enzymes potential therapeutic targets. The aim of this study was to assess and elucidate the anti-tumour effect and epigenetic regulatory mechanism of ribavirin in soft tissue sarcomas (STS). We showed that ribavirin inhibited growth and metastasis and prolonged survival in animals bearing STS cells by downregulating the mRNA and protein levels of PRMT1/PRMT5 and attenuating the accumulation of asymmetric and symmetric di-methylation of arginine (ADMA and SDMA).

Analysis of Key Control Points of Microbial Contamination Risk in Pork Production Processes Using a Quantitative Exposure Assessment Model.

Pork is one of the most common foods causing microbial foodborne diseases. Since pork directly enters the market after slaughtering, the control of microbial contamination in the slaughtering processes is the key to ensuring the quality and safety of pork. The contamination level of , a health-indicator bacterium, can reflect the risk level of potential pathogens.

Deep-learning geoacoustic inversion using multi-range vertical array data in shallow water.

A multi-range vertical array data processing (MRP) method based on a convolutional neural network (CNN) is proposed to estimate geoacoustic parameters in shallow water. The network input is the normalized sample covariance matrices of the broadband multi-range data received by a vertical line array. Since the geoacoustic parameters (e.

[Salidroside inhibits phenotypic transformation of rat pulmonary artery smooth muscle cells induced by hypoxia].

This study investigated the effect of salidroside on phenotypic transformation of rat pulmonary artery smooth muscle cells(PASMCs) induced by hypoxia. Rat pulmonary arteries were isolated by tissue digestion and PASMCs were cultured. The OD values of cells treated with salidroside at different concentrations for 48 hours were measured by cell counting kit-8(CCK-8) to determine the appropriate concentration range of salidroside.

Iron-Catalyzed Photoredox Functionalization of Methane and Heavier Gaseous Alkanes: Scope, Kinetics, and Computational Studies.

Herein, we report the development of the photocatalytic C-H functionalization of methane, ethane, and heavier gaseous alkanes with good yields and selectivity, broad scope (57 examples), mild conditions, and low cost. Kinetics and density functional theory calculations were investigated for the key photoinduced ligand-to-metal charge transfer and hydrogen atom transfer processes to reveal the detailed mechanism of iron photocatalysis. This work may bring novel ideas for feedstock upgrading and catalyst design.

deepMDDI: A deep graph convolutional network framework for multi-label prediction of drug-drug interactions.

It is crucial to identify DDIs and explore their underlying mechanism (e.g., DDIs types) for polypharmacy safety.

Plasma Concentration of Tumor Necrosis Factor-Stimulated Gene-6 as a Novel Diagnostic and 3-Month Prognostic Indicator in Non-Cardioembolic Acute Ischemic Stroke.

Background: Tumor necrosis factor-stimulated gene-6 (TSG-6) is a multifunctional, anti-inflammatory, and protective protein, while the association between TSG-6 and acute ischemic stroke (AIS) remains unclear in humans. This study aims to investigate the potential diagnostic and short-term prognosis predictive values of TSG-6 in non-cardioembolic AIS.

Methods: A total of 134 non-cardioembolic AIS patients within 24 h after AIS onset and 40 control subjects were recruited.

Plant Adaptability and Vegetation Differentiation in the Coastal Beaches of Yellow-Bohai Sea in China.

To identify the key soil factors influencing the vegetation differentiation in the coastal tidal flats of the Yellow-Bohai Sea in China, this study investigated the corresponding relationship between the (SA), (SS), and (PA) communities and their respective soil factors with published data, and combined the ecological strategy for analysis. The results showed a corresponding relationship between community and soil factors. The SA community had a lower bulk density (BD) and higher soil total nitrogen (TN), and the SS community was the opposite, while the PA community had the lowest salinity and higher TN.

Brain-wide ongoing activity is responsible for significant cross-trial BOLD variability.

A notorious issue of task-based functional magnetic resonance imaging (fMRI) is its large cross-trial variability. To quantitatively characterize this variability, the blood oxygenation level-dependent (BOLD) signal can be modeled as a linear summation of a stimulation-relevant and an ongoing (i.e.

[The application of NBI endoscopy in finding concealed primary lesions of misdiagnosis of oropharyngeal cancer].

The aim of this study is to analyze the application value of NBI endoscopy in finding the concealed primary lesions of misdiagnosis of oropharyngeal cancer. The clinical data of patients with missed oropharyngeal cancer treated in the Department of Otolaryngology Head and neck surgery, the Second Affiliated Hospital of Xi'an Jiaotong University from May 2018 to June 2021, were retrospectively studied, and the missed diagnosis was also analyzed combined with results of NBI endoscopy. In 31 cases of misdiagnosis of oropharyngeal cancer patients, including 25 males and 6 females, there was no significant difference in age, BMI index, course of disease and TNM stage （> 0.

Cortisol safeguards oogenesis by promoting follicular cell survival.

The role of glucocorticoids in oogenesis remains to be elucidated. cyp11c1 encodes the key enzyme involved in the synthesis of cortisol, the major glucocorticoid in teleosts. In our previous study, we mutated cyp11c1 in tilapia and analyzed its role in spermatogenesis.

Nuclear Targeted Peptide Combined With Gambogic Acid for Synergistic Treatment of Breast Cancer.

As a natural compound, gambogic acid (GA) emerged a shining multi-target antitumor activity in a variety of tumors. Whereas its poor solubility and non-specific effect to tumor blocked the clinical application of this drug. Herein, we reported a simple and effective strategy to construct liposome modified with nuclear targeted peptide CB5005N (VQRKRQKLMPC) polyethylene glycol (PEG) linker to decrease the inherent limitations of GA and promote its anti-tumor activity.

Gaining insight into the neural basis of resting-state fMRI signal.

The blood oxygenation level-dependent (BOLD)-based resting-state functional magnetic resonance imaging (rsfMRI) has been widely used as a non-invasive tool to map brain-wide connectivity architecture. However, the neural basis underpinning the resting-state BOLD signal remains elusive. In this study, we combined simultaneous calcium-based fiber photometry with rsfMRI in awake animals to examine the relationship of the BOLD signal and spiking activity at the resting state.

Tumor-microenvironmental-response Bi-functional molecules for efficient imaging and anti-tumor activity therapy.

To improve the visualization and potency of anticancer agents, the diagnosis and treatment integration bi-functional molecules were constructed based on active candidate BD7, approved drug Linifanib, and monoclonal antibody Bevacizumab. Commercial available Rhodamine B was inducted to realize imaging-aided diagnosis and target efficiency monitoring for cancer cells. In order to maintain the anticancer activity of drugs, disulfide bond was incorporated as releasable group based on tumor microenviroment.

Highly Selective Photoelectrochemical Assay of Arsenate Based on Magnetic CoO-FeO Cubes and the Negative-Background Signal Strategy.

Water pollution presents a significant environmental concern on earth. Herein, due to the serious environmental harmfulness of arsenate [As(V)], an iron phthalocyanine (FePc)-induced switchable photocurrent-polarity platform was developed for highly selective assay of As(V). First, magnetic CoO-FeO cubes were obtained by calcination of the CoFe Prussian blue analogue and then functionalized with oligonucleotide (S1).

Controlling the thermally activated delayed fluorescence of axially chiral organic emitters and their racemate for information encryption.

A pair of axially chiral organic enantiomers were facilely prepared through a one-pot sequential synthesis. They exhibit circularly polarized luminescence activities and have thermally activated delayed fluorescence (TADF) and aggregation-induced emission enhancement properties. Meanwhile, these two enantiomers present remarkable and reversible thermochromism in the crystalline state, enabling dual-colour TADF switching between orange and red.

Impact of Carbapenem Heteroresistance Among Multidrug-Resistant ESBL/AmpC-Producing Clinical Isolates on Antibiotic Treatment in Experimentally Infected Mice.

Purpose: Antibiotic resistance is a growing health crisis that is further complicated by treatment failures caused by bacteria that exhibit heterogeneous susceptibility to antibiotics. The aim of this study was to describe imipenem (IPM)-heteroresistant strains among multidrug-resistant (MDR) ESBL/AmpC-producing clinical isolates, investigate their molecular phenotypic characteristics, and elucidate the outcome of antibiotic treatment in mice infected with the heteroresistant isolates.

Materials And Methods: Antimicrobial susceptibility of isolates was determined by the disk diffusion and E-test methods.

Elicitation of ()-2-Hexenal and 2,3-Butanediol on the Bioactive Compounds in Adventitious Roots of var. .

This study aimed to investigate the elicitation of volatile organic compounds ()-2-hexenal and 2,3-butanediol on bioactive metabolites in var. adventitious root cultures by adding them into the medium. The experiment was performed for 72 h and the roots were dynamically sampled for quantification of representative astragaloside IV, calycosin-7--β-d-glucoside (CG), ononin, and the gene expression.

Rapid Selective Ablation and High-Precision Patterning for Micro-Thermoelectric Devices Using Femtosecond Laser Directing Writing.

Highly integrated miniature thermoelectric (TE) devices are desirable for applications of chip thermal management and self-powered energy harvesting. Currently, further performance improvement of micro-TE devices is largely limited by micro-nano-patterned processing, which shows the incompatibility with high-performance TE material fabrication or contradiction between machining accuracy and efficiency. This work presents a useful method to flexibly achieve high-precision array patterning for the micro-TE device through the femtosecond laser direct writing technique.

[Clinical features of diffuse large B-cell lymphoma in head and neck].

To investigate the clinical features of diffuse large B-cell lymphoma （DLBCL） of head and neck. A retrospective study was conducted among patients with DLBCL in the Department of otolaryngology and head and neck surgery of the Second Affiliated Hospital of Xi'an Jiaotong University from July 2011 to September 2021. The disease location, clinical manifestations, diagnosis, treatment and prognosis of DLBCL patients in head and neck were analyzed retrospectively.

Exosomes derived from hepatitis B virus-infected hepatocytes promote liver fibrosis via miR-222/TFRC axis.

Exosomal miRNAs activates hepatic stellate cell (HSC) and promote fibrosis. miR-222 was found to be increased in hepatitis B virus (HBV)-infected hepatocytes, and ferroptosis was reported to ameliorate liver fibrosis (LF). Although miR-222 and ferroptosis have been implicated in LF, the association between miR-222 and ferroptosis and how they coordinate to regulate LF are still not explicit.

Conformational Changes in a Macrolide Antibiotic Binding Protein From Upon ADP Binding.

Rv3197 (MABP-1), a non-canonical ABC protein in , has ATPase activity and confers inducible resistance to the macrolide family of antibiotics. Here we have shown that MSMEG_1954, the homolog of Rv3197 in , has a similar function of conferring macrolide resistance. Crystal structures of apo-MSMEG_1954 (form1 and form 2) and MSMEG_1954 in complex with ADP have been determined.

Effects of incretin-based therapies on β-cell function in type 1 diabetes mellitus: a systematic review and meta-analysis.

Aim: To assess the effects of incretin-based therapies on β-cell function in patients with type 1 diabetes mellitus (T1DM).

Methods: We searched the PubMed, Cochrane Library, Embase, and Web of Knowledge databases for eligible randomized clinical trials published up to July 2021. The inclusion criteria were patients with T1DM or latent autoimmune diabetes in adults, patients treated with dipeptidyl peptidase-4 inhibitors or glucagon like peptide-1 receptor agonists, and outcomes included one of the following: fasting plasma glucose, fasting C-peptide, postprandial C-peptide, C-peptide area under the curve (AUC), homeostasis model assessment for β cell function, and insulin resistance.

Ribavirin inhibits the growth and ascites formation of hepatocellular carcinoma through downregulation of type I CARM1 and type II PRMT5.

Type I co-activator-associated arginine methyltransferase 1 (CARM1) and type II protein arginine methyltransferase 5 (PRMT5) are highly expressed in multiple cancers including liver cancer and their overexpression contributes to poor prognosis, thus making them promising therapeutic targets. Here, we evaluated anti-tumor activity of ribavirin in hepatocellular carcinoma (HCC). We found that ribavirin significantly inhibited the proliferation of HCC cells in a time- and dose-dependent manner.

[Effect of proton pump inhibitort on salivary pepsin concentration in patients with laryngopharyngeal reflux].

To investigate the effect of proton pump inhibitor（PPI） treatment on salivary pepsin concentration in laryngopharyngeal reflux（LPR）. 152 patients with suspected LPR complaining non-specific symptoms such as foreign body sensation, dry throat, phlegm and other non-specific symptoms were enrolled， in the Second Affiliated Hospital of Xi'an Jiaotong University from August 2019 to December 2020. According to the scores of reflux symptom index（RSI） and reflux finding score（RFS）, all the patients were divided into LPR （+） group and LPR （-） group, RSI （+） group and RSI （-） group, RFS （+） group and RFS （-） group .